Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode

A technology for modifying electrodes and electrodes, which is applied in the field of biomedical detection and can solve problems such as small detection range

Active Publication Date: 2020-12-01
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The invention patent with the publication number CN109633174A discloses a C-reactive protein ultrasensitive detection method based on a biotin quantum dot probe. The time required for the whole set of detection is 110 min, and the detection is relatively time-consuming
The invention patent with the publication number CN 109946273A discloses a fluorescent probe for detecting C-reactive protein and its preparation method. The detection concentration range is 0.39μg / mL~50μg / mL, and the detection range is relatively small

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  • Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode
  • Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode
  • Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode

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Embodiment Construction

[0030] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] A method for electrochemical detection of C-reactive protein based on Fc-ECG / MEL / AuNPs / SPE modified electrode, the detection principle is shown in figure 1 . Using the layer-by-layer self-assembly method, firstly, the electrode is pretreated with SPE as the substrate of the sensor; secondly, the treated electrode is soaked in HAuCl 4In this method, a layer of AuNPs particles was formed on the surface of the working electrode of SPE by the constant potential deposition method; then MEL was added dropwise on the surface of AuNPs / SPE and dried at room temperature, and the aggregation of dispersed AuNPs particles was induced by covalent bonding, so that MEL is attached to AuNPs to form MEL / AuNPs / SPE; Fc-ECG electroactive material is added dropwise on the surface of MEL / AuNPs / SPE, and there is a hydrogen bond force between the amino group...

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Abstract

The invention discloses a method for electrochemically detecting C-reactive protein based on an Fc-ECG / MEL / AuNPs / SPE modified electrode, and a new electrochemical sensor Fc-ECG / MEL / AuNPs / SPE is manufactured by adopting an electrodeposition technology and an electrostatic adsorption effect and is used for detecting the C-reactive protein. An SPE is taken as a substrate, ferrocenyl formylglutation (Fc[CO-Glu-Cys-Gly-OH], Fc-ECG) is used as an electrochemical probe and a recognition element, and has a specific recognition effect on CRP, and by means of the synergistic effect of melamine and goldnanomaterials, electrochemical signals are doubly enhanced to construct the sensor, and the CRP is detected by using a DPV method. The method is simple to operate, time-saving, low in cost and relatively low in detection limit.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a method for electrochemically detecting C-reactive protein. Background technique [0002] C-reactive protein (C-reactive protein, CRP) is an important clinical biomarker for diseases such as inflammation, cardiovascular, coronary heart disease, and sepsis. Sensitive, selective, and simple detection of CRP is of great significance. CRP detection methods include one-way immunodiffusion method, latex coacervation method, immune scattering turbidimetry, latex enhanced immune transmission turbidimetry, immunolabeling technology, enzyme-linked immunosorbent assay, etc. The detection principle is to use specific anti-CRP antibody and detection method. According to the CRP reaction in the sample, determine the CRP content in the test sample according to the diameter of the formed precipitation ring, the height of the precipitation peak, the degree of agglutinati...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 韩国成詹涛何彦龙冯小珍陈真诚周治德
Owner GUILIN UNIV OF ELECTRONIC TECH
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