A SNP marker closely linked to the major qtl for leaf width under continuous multi-leaf tassel in maize and its application

Abstract

The invention relates to the technical fields of genetic engineering and molecular biology, in particular to a SNP marker closely linked with the main QTL of the continuous multi-leaf position under the maize tassel and its application. The closely linked SNP marker of the major QTL is chr2_208336, which is mutated to A / G, and the physical position refers to the reference genome B73 RefGen_v4 version of the maize inbred line B73. The SNP marker is used for assisted selection, and it is only necessary to detect the SNP base at the specific site of the PCR amplification product of the primer to predict the width of the continuous four-leaf width trait under the maize tassel at the mature stage. The identification method is simple and the selection efficiency is high. Before sowing or in the early growth stage of maize, the continuous four-leaf leaf width and leaf narrow maize plant under the mature tassel can be identified, which can be used for narrow-leaf density-tolerant maize breeding and wide-leaf silage maize breeding, respectively. The selection target is clear. And it is not affected by the environment, which provides a feasible way for molecular-assisted breeding of narrow-leaf silage maize and broad-leaf silage maize.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and molecular biology, and in particular relates to a SNP marker closely linked with the major QTL of continuous multi-leaf position and leaf width under the maize tassel and its application. Background technique [0002] Corn is the main food and feed and industrial raw material crop. In 2014, it became the crop with the largest planting area in China, and it occupies a very important position in China's agricultural production and national economic development. [0003] It has been proved by breeding practice that the increase of maize yield per unit area is due to the increase of maize tolerance to close planting (Duvick, D., 2005 Genetic progress in yield of United States maize (Zea mays L.). Maydica 50: 193.). However, under high-density planting conditions, the leaves will shield each other, especially the wider leaves at the upper part, which will seriously affect the ventilat...

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Problems solved by technology

Some studies on the leaf width traits of different leaf positions in maize have also shown that the leaf width of different leaf positions is regulated by one or several common QTLs, and there are also different QTLs. The genetic basis is very complicated.

Tian(Tian,F.,P.J.Bradbury,P.J.Brown,H.Hung,Q.Sun et al.,2011Genome-wide association study of leafarchitecture in the maize nested association mapping population.Nat Genet 43:159-162.) and Yang( Yang, N., Y.Lu, X.Yang, J.Huang, Y.Zhou et al., 2014 Genome wide association studies using a new nonparametric model reveal the genetic architecture of 17agronomic traits in an enlarged maize association panel.PLoSGenet10:e1004573.) etc. A number of SNP loci that were significantly associated with leaf width were identified by association analysis, and they were distributed on multiple chromosomes in maize. Study on Positioning of Leaf Width in Multi-leaf Position

In addition, limited by the type of mapping population, the type of markers used, the accuracy of the genetic linkage map, and environmental control, the QTL analysis of maize leaf width, especially the continuous multi-leaf position under the tassel, has no consistent results. Application of QTL Markers for Leaf Width at Multi-leaf Position Under Panicle

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