Lipase determination kit and preparation method thereof

A kit and lipase technology, applied in the field of clinical biochemical detection, can solve the problems affecting the batch difference and stability of reagents, increase the workload of clinical detection, and narrow the linear range, so as to improve solubility and dissolution efficiency, and enhance stability. The effect of improving the stability and linear range

Active Publication Date: 2020-12-08
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
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Problems solved by technology

However, in the preparation process of the R2 reagent, there will be insufficient dissolution of the chromogen substrate 1,2-o-dilauryl-racem-glycerol-3-pentanoate-(6-methylresorufin) ester, which will affect The batch-to-batch difference and stability of the reagent, and as the storage time increases, there will be substra

Method used

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  • Lipase determination kit and preparation method thereof
  • Lipase determination kit and preparation method thereof
  • Lipase determination kit and preparation method thereof

Examples

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Effect test

Embodiment 1

[0026] This embodiment provides a lipase assay kit, including R1 reagent and R2 reagent, the pH of the R1 reagent is 8.0, specifically including:

[0027]

[0028] The pH of the R2 reagent is 4.0, specifically including

[0029]

[0030] Wherein, the content percentages of the components in the R1 reagent and the R2 reagent are both mass percentages, and the stabilizer is mercaptoethanol and mannitol with a mass ratio of 1:2.

[0031] The preparation method of the kit is as follows: (1) Preparation of R1 reagent: Measure an appropriate amount of pure water, add BICINE buffer solution, then adjust the pH to 8.0, then add other components in the R1 reagent according to the above ratio, stir and dissolve Constant volume is obtained described R1 reagent; (2) The preparation of R2 reagent: 1. Weigh the substrate 1,2-o-dilauryl-racem-glycerol-3-pentanoic acid-(6-methyl resorufin) Put it into a beaker filled with ethyl acetate, stir well, then add dilaurin sulfate and Tritonx-...

Embodiment 2

[0033] The difference between this embodiment and embodiment 1 is: the formula of the R1 reagent is:

[0034]

[0035] The pH of the R2 reagent is 4.0, specifically including

[0036]

[0037] Wherein, the content percentages of the components in the R1 reagent and the R2 reagent are both mass percentages, and the stabilizer is mercaptoethanol and mannitol with a mass ratio of 1:2.

Embodiment 3

[0039]The difference between this example and Example 1 is that the stabilizer in the R2 reagent is mercaptoethanol and mannitol with a mass ratio of 1:1.

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Abstract

The invention discloses a lipase determination kit and a preparation method thereof. An R1 reagent comprises a buffer solution, sodium deoxycholate, colipase, metal salt, a protective agent, a surfactant and a preservative; an R2 reagent comprises Ltartaric acid, calculus bovis deoxycholic acid sodium salt, 1, 2-o-dilauroyl racemic glycerol 3valeric acid (6methyl resorufin) ester, a cosolvent, a stabilizer and a preservative, and the cosolvent is composed of ethyl acetate, dilauroyl glycerol sulfate and a Triton surfactant. According to the invention, through optimization, ethyl acetate, dilauryl glyceryl sulfate and Triton surfactant are jointly used as a cosolvent to play a synergistic effect of the three components, so that the solubility of a substrate is greatly improved, and further,mercaptoethanol and mannitol are used as composite stabilizers, therefore, the lipase determination kit which is good in stability, high in accuracy and precision and remarkably improved in linear range is obtained.

Description

technical field [0001] The invention belongs to the technical field of clinical biochemical detection, and in particular relates to a lipase assay kit and a preparation method thereof. Background technique [0002] Serum lipase (LPS) is mainly derived from the pancreas, and a small amount is derived from the stomach and small intestine. The determination of lipase activity is used to reflect the disorder of the pancreas. Increased human lipase is common in acute pancreatitis, pancreatic cancer, pancreatic duct obstruction due to stones, liver cirrhosis, intestinal obstruction, acute and chronic kidney disease, etc. LPS is a group of enzymes that catalyze the hydrolysis of long-chain fatty acid glycerides, which only hydrolyzes the α-position fatty acids of fatty acid glycerides. LPS has a certain specificity for long-chain fatty acid glycerides, and the maximum activity and specificity of LPS requires the participation of cholate and co-lipase. Chromogen substrate method a...

Claims

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Application Information

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IPC IPC(8): G01N31/10
CPCG01N31/10
Inventor 凡速朋赵畅龚婷梁艳吴年芬舒芹张雪娇赵愿安
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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