Application of cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light

A technology of flora balance and flower extraction, applied in the application field of cherry blossom flower extract in maintaining skin flora balance and/or anti-blue light, and can solve problems such as no relevant reports on the effect.

Active Publication Date: 2020-12-18
GUANGDONG HEJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the role of cherry blossom extracts, especially

Method used

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  • Application of cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light
  • Application of cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light
  • Application of cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The third aspect of the present invention provides a method for preparing cherry blossom extract, the method comprising the following steps:

[0032] (1) After the dried cherry blossom material is pulverized, the pulverized cherry blossom material is dissolved in an organic solution and extracted to obtain an extract;

[0033] (2) Concentrating the extract obtained in step (1) and removing the organic solvent to obtain a cherry blossom extract concentrate, and then drying the concentrate to obtain a cherry blossom extract.

[0034] According to a preferred embodiment of the present invention, wherein the dried cherry blossom flower material in step (1) is selected from dried cherry blossom flowers and / or dried cherry blossom petals, preferably dried cherry blossom petals.

[0035] Preferably, in the dried cherry blossom material, based on the total weight of the dried cherry blossom material, the moisture content is 0.5-10% by weight.

[0036] Any existing dry cherry b...

Embodiment 1

[0065] This example is used to illustrate the anti-blue light effect of cherry blossom flower extract in vitro experiments.

[0066] (1) Analysis of cell apoptosis by flow cytometry and Annexin V-FITC / PI double staining

[0067] Take the HDF cells in the logarithmic growth phase at 25cm 2 Culture flasks were cultured with DMEM complete medium (containing 10 volume % serum and 1 volume % penicillin and streptomycin) (conditions: 37 °C, 5% CO 2 ), remove the culture medium when the cell density reaches 60-70% of the bottom area of ​​the bottle, wash with sterile PBS twice and then remove to remove the residual culture medium.

[0068] Experimental group: add 2 mL of cherry blossom flower extract solution (the above cherry blossom flower extract powder solution prepared with water, the concentration is 5% by weight), and incubate at 37° C. for 24 hours.

[0069] Control group: Add 2 mL of serum-free 1640 medium and incubate at 37°C for 24 hours.

[0070] The cells of the exper...

Embodiment 2

[0084] This example is used to illustrate the effect of cherry blossom flower extract on maintaining the balance of skin flora in rat experiments.

[0085] The rats were divided into three groups: experimental group, positive control group, and blank control group, with 6 rats in each group. Among them, the rats in the experimental group and the positive control group were subjected to skin flora balance destruction modeling, and the blank control group was not subjected to any treatment. The specific modeling method is: apply coal tar to the opening of the ear canal of the left ear of the rat and inject it subcutaneously at a concentration of 5×10 12 CFU / L of Staphylococcus aureus (S.aureus) bacterial fluid to establish a model of skin flora balance disruption.

[0086] After the successful modeling, the rats in the experimental group and the positive control group were treated with the cherry blossom flower extract and fusidi lactic acid ointment prepared by the above metho...

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Abstract

The invention relates to the technical field of skin care product research and development, and discloses an application of a cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light. The fact that the cerasus sp. flower extract has the efficacy of maintaining skin flora balance and/or resisting blue light is found for the first time, and the specific effects of thecerasus sp. flower extract are verified through in-vitro experiments and animal experiments. By the technical scheme, the blank of the application of the cerasus sp. flower extract to maintaining skin flora balance and/or resisting blue light is filled up, and reference is provided for research and development of skin care products with related efficacy.

Description

technical field [0001] The invention relates to the technical field of research and development of skin care products, in particular to the application of cherry blossom flower extracts in maintaining the balance of skin flora and / or anti-blue light. Background technique [0002] Skin maintenance is a new concept of skin care proposed in recent years. Its core concept is to maintain "skin stability". The so-called stability is not "unchanged", but in a "dynamic balance" according to changes in the external environment, one's own physiology and psychology. "status. "Stable" skin means that it is not easy to have obvious adverse reactions (such as allergies, redness, dry skin, excessive oil secretion, etc.), even if it is stimulated, it can recover quickly, and it is not easy to fluctuate repeatedly. Studies have shown that the skin has its own unique flora microecology, and there is a mutually beneficial symbiotic relationship between the resident flora of the skin and betwe...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/00A61Q17/04
CPCA61K8/9789A61Q19/00A61Q17/04A61K2800/84Y02A50/30
Inventor 张文环屈恋张文云罗剑芳张兵伍宇飞艾勇何廷刚
Owner GUANGDONG HEJI BIOTECH CO LTD
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