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Composite microbial preparation containing bacillus subtilis

A Bacillus subtilis and compound microorganism technology, applied in the field of microbial preparations, can solve the problems of low sporulation rate, low Bacillus subtilis number, affecting the quality of microbial preparations and the amount of additions, etc., so as to reduce the ratio of feed to meat, improve disease resistance and immunity energy, reduced feed consumption and medication expenditure

Active Publication Date: 2020-12-18
安徽希普生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention is a single-type Bacillus subtilis feed additive. Although it can improve the nutritional value of the Bacillus subtilis additive and promote the metabolism of animals, the number of bacteria in the Bacillus subtilis is low in the production process, especially the low spore rate. It greatly affects the quality of the prepared microbial preparation and the amount added in the subsequent feed

Method used

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  • Composite microbial preparation containing bacillus subtilis

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Embodiment 1

[0036] Embodiment 1 A kind of composite microbial preparation containing bacillus subtilis

[0037] The composite microbial preparation containing Bacillus subtilis consists of the following components and parts by weight: 40 parts of composite microbial powder, 20 parts of composite carrier, and 2 parts of protective agent.

[0038] The preparation method of described compound microbial bacterial powder, comprises the steps:

[0039] S1. Inoculate the activated Bacillus subtilis, Bacillus cereus and Lactobacillus inulin into the seed culture medium respectively, the inoculation amount is 3%, the cultivation temperature is 34°C, the shaking table speed is 160r / min, and cultivated for 12h. Obtain Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed liquid;

[0040] The seed medium is 0.3% beef extract, 1% peptone, 2% glucose, 0.5% NaCl, added water to 100%, pH 5.

[0041] S2. Mix and inoculate the Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed so...

Embodiment 2

[0050] Embodiment 2 A kind of compound microbial preparation containing bacillus subtilis

[0051] The compound microbial preparation containing Bacillus subtilis consists of the following components and parts by weight: 50 parts of compound microbial powder, 25 parts of compound carrier, and 5 parts of protective agent;

[0052] The preparation method of described compound microbial bacterial powder, comprises the steps:

[0053] S1. Inoculate the activated Bacillus subtilis, Bacillus cereus and Lactobacillus inulin into the seed culture medium respectively, the inoculation amount is 6%, the cultivation temperature is 35°C, the shaking table speed is 180r / min, and cultivate for 12h, Obtain Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed liquid;

[0054] The seed medium is 0.3% beef extract, 1% peptone, 2% glucose, 0.5% NaCl, added water to 100%, pH 5.

[0055] S2. Mix and inoculate the Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed solutio...

Embodiment 3

[0064] Embodiment 3 A kind of composite microbial preparation containing bacillus subtilis

[0065] The composite microbial preparation containing Bacillus subtilis consists of the following components and parts by weight: 60 parts of composite microbial powder, 30 parts of composite carrier, and 10 parts of protective agent.

[0066] The preparation method of described compound microbial bacterial powder, comprises the steps:

[0067] S1. Inoculate the activated Bacillus subtilis, Bacillus cereus and Lactobacillus inulin into the seed culture medium respectively, the inoculation amount is 10%, the cultivation temperature is 36°C, the shaking table speed is 200r / min, and cultivated for 12h. Obtain Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed liquid;

[0068] The seed medium is 0.3% beef extract, 1% peptone, 2% glucose, 0.5% NaCl, added water to 100%, pH 5.

[0069] S2. Mix and inoculate the Bacillus subtilis, Bacillus cereus and Lactobacillus inulin seed ...

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Abstract

The invention belongs to the technical field of microbial preparations, and particularly relates to a composite microbial preparation containing bacillus subtilis. The composite microbial preparationcontaining the bacillus subtilis comprises the following components in parts by weight: 40-60 parts of composite microbial powder, 20-30 parts of a composite carrier and 2-10 parts of a protective agent. According to the composite microbial preparation containing the bacillus subtilis, through the mutual coordination effect of bacillus subtilis, bacillus cereus and inulin bacillus, the side effects caused by antibiotic abuse are overcome, and a safe and green composite microbial preparation is provided for human beings; and meanwhile, the number of spores generated in the biological bacteriumfermentation process is effectively increased, and an important guarantee is provided for effectively prolonging the service life of the microbial agent and maintaining the pesticide effect and stability of the microbial agent in the subsequent preparation process of the biological bacterium preparation.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, in particular to a composite microbial preparation containing Bacillus subtilis. Background technique [0002] Piglet digestive tract diseases seriously restrict the development of healthy pig breeding ("The Epidemic Status and Prevention and Control Measures of Pig Systemic Diseases", Chen Huanchun, Veterinary Guide, Issue 11, 2017, Page 6, Abstract Line 1, Publication Day June 30, 2017). According to statistics, the annual incidence of digestive tract diseases in piglets is as high as 50.47%, the fatality rate of bacterial diseases reaches more than 50%, and the fatality rate of viral diarrhea can reach 100%. In the past 30 years, significant breakthroughs have been made in the detection, prevention and control of piglet digestive tract diseases in my country, but the results are not satisfactory. Livestock and poultry diseases continue to break out and new diseases continue to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10A23K10/18A23K50/30A23K50/60C12R1/125C12R1/085C12R1/07
CPCA23K10/18A23K50/30A23K50/60C12N1/20C12N11/10
Inventor 恽辉余忠丽赵大伟王俊青劳泰财
Owner 安徽希普生物科技有限公司
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