Temperature measurement method based on rare earth luminescence lifetime temperature probe

Abstract

The invention discloses a temperature measurement method of an internal quenching type rare earth luminescence lifetime temperature probe, and the method realizes temperature detection based on fluorescence lifetime change. The center of an adopted material is a nano core doped with rare earth trivalent ions Yb<3+> and B<3+>, the periphery of the material is an inert shell layer, a phonon auxiliary energy transfer process exists between the Yb<3+> and the B<3+> rare earth ions, and the service life of the rare earth ions is changed along with the change of temperature due to the fact that phonons participate in the energy transfer process. The inert shell layer can play a role in protecting the service life of the light-emitting core from changing along with the change of the environment (except the temperature), the light-emitting waveband is in the 700-1700nm region, and the water solubility can be improved by using a ligand to modify the surface. The near-infrared luminescence lifetime temperature probe can realize accurate temperature measurement in different complex environments (including living bodies), and has the advantages of internal quenching temperature sensitivity, near-infrared band luminescence, nanoscale, accuracy, quantifiability, good stable dispersibility and the like.

Description

technical field [0001] The invention relates to the field of luminescent nanometer probes and their applications, in particular to a temperature measurement method based on a rare earth luminescence lifetime temperature probe. Background technique [0002] Life activities in nature are always accompanied by changes in temperature, and during the evolution of organisms, the tolerance of different organisms to temperature is also quite different. For example, psychrophilic bacteria can survive at minus 15°C, while thermophilic bacteria can survive at temperatures as high as 110°C. For mammalian warm-blooded animals, metabolism and nerve signal transmission are always accompanied by temperature changes, while cells are a tiny unit that reflects temperature regulation, and the functions of many cells reflect the macroscopic temperature of living bodies. Finally, it can resist temperature changes within a certain range externally, and maintain a normal body temperature within a ...

AI Technical Summary

Problems solved by technology

However, because the fluorescence signals of different bands are absorbed and scattered by different tissues, in different complex environments, the degree of attenuation of the fluorescence signals of different bands of the specific temperature probe is also different, thus affecting the intensity of the fluorescence signal. accurate readout of

[0004] At present, the research and development of luminescent probes based on the luminescence lifetime as a temperature detection signal is very little. Some researchers use nano-gold clusters as temperature probes. The fluorescence lifetime of gold clusters is greatly affected by the external environment other than temperature, and cannot accurately respond. The temperature of the environment and its lifetime are at the nanosecond level, so the requirements for both the excitation light and the detector are relatively high

Images