Method for preparing white-rot fungus solid culture medium using chrome leather shavings and application

Abstract

The invention belongs to the field of leather solid wastes, and particularly relates to a method for preparing a white-rot fungus solid culture medium using chrome leather shavings and application. The method comprises the following steps of: firstly, preparing dechromed hydrolyzed collagen by using tanning waste chrome leather shavings as a raw material and adopting a batch feeding process basedon an alkaline enzyme hydrolysis system; secondly, compounding glucose, a small amount of peptone, a trace element solution, agar, vitamin B1 and purified water by taking the extracted dechromed hydrolyzed collagen as a nutrient source to prepare a solid fermentation culture medium; and finally, fermenting and culturing white-rot fungi by utilizing a solid culture medium. The prepared solid culture medium can achieve normal and efficient growth of the white-rot fungi, not only achieves the purpose of changing the waste chrome leather shavings into valuables, but also can greatly replace traditional peptone resources, effectively reduces the culture cost of the white-rot fungi, and is beneficial to high-valued utilization of chrome-protein-containing solid wastes.

Description

technical field

[0001] The invention belongs to the field of solid leather wastes, and in particular relates to a method and application for preparing a solid medium for white-rot fungi by using chrome leather shavings. Background technique

[0002] Since the 1990s, the leather industry has developed rapidly, and China has gradually become the largest leather-making country in the world, but the accompanying pollution problems have also become increasingly serious. Especially in the leather production process, 70% of the wastes are chromium-containing wastes, which mainly include chrome leather shavings, chrome-containing leather pieces, grinding leather ash, etc. Nitrogen sources, on the other hand, are the nutrients that constitute the source of nitrogen in microbial cells and metabolites. Tannery waste is rich in collagen and its hydrolysis products (polypeptides, amino acids, etc.) in different degrees, which can provide the necessary organic nitrogen source for the gro...

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