Selenium-enriched hericium erinaceus mycelium beverage and preparation method thereof
A technology of mycelium and Hericium erinaceus, applied in the field of food processing, can solve the problems of easy layering, light taste, uneven color, etc.
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Embodiment 1
[0038] Embodiment 1: the preparation method of selenium-rich Hericium erinaceus mycelium beverage, comprises the steps:
[0039] (1) The strain of Hericium erinaceus in the presence of 10 μg / mL Na 2 SeO 3 The mycelium obtained after culturing the solid PDA medium at 26°C for 7 days was inoculated in 10 μg / mL Na 2 SeO 3 The liquid seed liquid culture medium, the liquid seed liquid medium composition is potato 20%, glucose 1%, maltose 1%, MgSO 4 0.15%, K 2 HPO 4 0.1%, natural pH, 26°C, 250rpm shaking culture for 8 days, inoculated in a medium containing 10μg / mL Na 2 SeO 3 , 4.6% soluble starch, 4.6% soybean flour, 0.03% CaCl 2 , 24mg / 100mL VB 1 +VB 2 +VB 6 In the shake flask of the liquid medium, the inoculum amount is 10%, 26°C, 250rpm shaking culture for 6 days, centrifugation or filtration, the mycelium is washed with deionized water, dried to obtain the selenium-enriched Hericium erinaceus mycelium, organic The selenium content is 162.3mg / kg, and the dry weight ...
Embodiment 2
[0044] Embodiment 2: the preparation method of selenium-rich Hericium erinaceus mycelium beverage, comprises the steps:
[0045] (1) The strain of Hericium erinaceus was mixed with 12 μg / mL Na 2 SeO 3 The mycelium obtained after culturing the solid PDA medium at 26°C for 7 days was inoculated in 12 μg / mL Na 2 SeO 3 The liquid seed liquid culture medium, the liquid seed liquid medium composition is potato 20%, glucose 1%, maltose 1%, MgSO 4 0.15%, K 2 HPO 4 0.1%, natural pH, 26°C, 250rpm shaking culture for 7 days, inoculated in a medium containing 12μg / mL Na 2 SeO 3 , 5.0% soluble starch, 5.0% soybean flour, 0.02% CaCl 2 , 26mg / 100mL VB 1 +VB 2 +VB 6 In a shake flask of liquid medium, the inoculum amount is 8%, 28°C, 250rpm shaking culture for 8 days, centrifuged or filtered, the mycelium is washed with deionized water, dried to obtain the selenium-enriched Hericium erinaceus mycelium, organic The selenium content is 189.5mg / kg, and the dry weight of mycelium is 1...
Embodiment 3
[0050] Embodiment 3: the preparation method of selenium-rich Hericium erinaceus mycelium beverage, comprises the steps:
[0051] (1) The strain of Hericium erinaceus was mixed with 16 μg / mL Na 2 SeO 3 The mycelium obtained after culturing the solid PDA medium at 26°C for 6 days was inoculated in 16 μg / mL Na 2 SeO 3 The liquid seed liquid culture medium, the composition of the liquid seed liquid medium is potato 18%, glucose 1.2%, maltose 1.2%, MgSO 4 0.12%, K 2 HPO 4 0.1%, natural pH, 26°C, 250rpm shaking culture for 8 days, inoculated in a medium containing 16μg / mL Na 2 SeO 3 , 4.8% soluble starch, 4.8% soybean flour, 0.03% CaCl 2 , 24mg / 100mLVB 1 +VB 2 +VB 6 In the fermenter of the liquid medium, the inoculation amount is 10%, 26°C, dissolved oxygen 20-80%, fermented for 6 days, centrifuged or filtered, the mycelium is washed with deionized water, dried to obtain selenium-enriched Hericium erinaceus Mushroom mycelium, the content of organic selenium is 180.6mg / k...
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