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A preparation method and application of a microneedle swab capable of spontaneously enriching viruses

A microneedle and virus technology, applied in the field of biomedical detection, can solve the problem of inability to improve virus sampling rate and accuracy, and achieve the effect of improving detection efficiency and accuracy, good repeatability and good biocompatibility

Active Publication Date: 2021-08-06
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method and application of a microneedle swab capable of spontaneously enriching viruses, so as to solve the problem that the existing common swabs in the background technology cannot improve the virus sampling rate and accuracy
[0011] In order to realize the above-mentioned method for preparing a microneedle swab capable of spontaneously enriching viruses and its application to solve the problem that the existing common swab cannot improve the virus sampling rate and accuracy proposed in the background art, the present invention provides the following technical solutions:

Method used

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  • A preparation method and application of a microneedle swab capable of spontaneously enriching viruses
  • A preparation method and application of a microneedle swab capable of spontaneously enriching viruses
  • A preparation method and application of a microneedle swab capable of spontaneously enriching viruses

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preparation example Construction

[0035] The invention provides a method for preparing a microneedle swab capable of spontaneously enriching viruses, comprising the following steps:

[0036] (1) Preparation of microneedle molds: use graphics software to design the original microneedle model, and use polydimethylsiloxane (PolydimethylSiloxane, PDMS) to construct a microneedle mold for preparing microneedles; wherein, the microneedle mold The height of the microneedle to be prepared in the design is 50 microns to 1 mm, the diameter of the bottom of the microneedle to be prepared in the design of the microneedle mold is 50 microns to 1 mm, and the number of microneedle arrays to be formed in the design of the microneedle mold is 1 to 500 pieces.

[0037] (2) Loading of virus antibody: configure the virus antibody solution, and add it into the microneedle mold, then act in a vacuum environment, and use a horizontal rotor centrifuge to centrifuge, after repeated repetitions (the number of repetitions is 1-20 times)...

Embodiment 1

[0042] The specific preparation method steps of the microneedle swab capable of spontaneously enriching viruses of the present invention are as follows:

[0043] 1. Loading of virus antibody

[0044] Use solidworks and other software to design the microneedle matrix, and use the Lite600HD 3D printer to print out the mold with white resin. A microneedle mold with a cavity was constructed using a PDMS commercial kit. After being fixed in a 3.5mm cell culture, add 200 microliters of 1 mg / ml new coronavirus surface spike protein antibody, apply vacuum for 10 minutes at about 2.5KPa atmospheric pressure, and centrifuge using a horizontal rotor centrifuge (2000 rpm, 20 minutes) ). After the liquid flows into the cavity of the mold, the antibody solution is replenished, and the vacuum is applied again for 10 minutes, and the above process is repeated 3 times.

[0045] 2. Construction of microneedle matrix

[0046] After loading the new coronavirus antibody, prepare 2% sodium algi...

Embodiment 2

[0050] The present invention also provides the application of the microneedle swab prepared by the method for preparing the microneedle swab capable of spontaneously enriching viruses in respiratory tract and oral cavity diseases. The specific medical applications of the present invention will be described below through specific embodiments in conjunction with the accompanying drawings.

[0051] Such as figure 1 Shown is a schematic diagram of microneedle construction and antibody loading with different degrees of cross-linking. In order to achieve a higher efficiency of virus sampling, two microneedle films with different cross-linking degrees were constructed ( figure 1 A): The microneedle film with a low degree of cross-linking is easy to load antibodies, and it is easier to induce virus aggregation and penetration; the microneedle film with a high degree of cross-linking can provide higher mechanical strength and tissue penetration ability, which is helpful Inducing micr...

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Abstract

The invention discloses a preparation method and application of a microneedle swab capable of spontaneously enriching viruses, comprising the following steps: (1) preparation of a microneedle mold; (2) loading of virus antibodies: preparing a virus antibody solution, and adding it into the microneedle mold; (3) construction of microneedle array; (4) construction of virus-enriched microneedle swab. The present invention utilizes minimally invasive microneedle technology combined with specific germ antibodies to construct a microneedle system capable of spontaneously enriching germs, and assembles it to the end of a medical swab. The constructed microneedle swab can penetrate the oral mucosa and reach the deep tissue, and through the interaction of antigens and antibodies, it can capture germs more efficiently, increase the difference between negative samples and positive samples, and reduce false negatives in the detection process result. The method provided by the invention is simple, efficient, has good biocompatibility and repeatability, improves the detection efficiency and accuracy of germs, and has high potential for clinical transformation.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to a preparation method and application of a microneedle swab capable of spontaneously enriching viruses. Background technique [0002] Sampling is the first step in disease detection, and it is also a very critical step. Taking the detection of the novel coronavirus that broke out globally in 2019 as an example, oropharyngeal swabs and nasopharyngeal swabs are the main sampling methods. The doctor inserts the swab into the oropharynx or nasopharynx of the tester, picks up a small amount of secretion, stores it in the virus preservation solution, and then places it in a temperature-controlled device for cultivation. After extracting the RNA, the nucleic acid sequence is performed. Detection (fluorescent quantitative PCR, etc.). The accuracy of the sampling method directly determines the test results. If the sampling method is improper, it may lead to wrong results and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6804C12R1/93
CPCA61B10/00C12Q1/6804C12Q1/701C12Q2563/131
Inventor 陈伟王琳王征陈小元蔡博耿帜陈凤花
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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