Photoacoustic response type editable nanoprobe and preparation method and use thereof
A nano-probe and acoustic response technology, which is applied in the field of nano-biology, can solve the problems of photodynamic and sonodynamic therapy, such as long-term reduction, and achieve the effects of editable diagnosis and treatment functions, stable physical and chemical structures, and broad application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0040] The embodiment of the present invention also provides a method for preparing a photoacoustic responsive editable nano-diagnosis probe, the method comprising the following steps:
[0041]Step 1, preparation of hypericin and 1,3-diglycidyl ether glycerylamine mixture: 1,3-diglycidyl ether glycerylamine was added to 0.5mL Tris-HCl buffer to obtain glycidol The tris-HCl dispersion liquid of ether glycerylamine, then the tris-HCl dispersion liquid of glycidyl ether glycerylamine and the DMSO dispersion liquid of hypericin are mixed, heated and stirred to obtain the first dispersion liquid;
[0042] Specifically, the concentration of 1,3-diglycidyl ether glycerylamine dispersion is 0.1-1M; the concentration of hypericin dispersion is 0.01-1M; hypericin and 1,3-diglycerol In the mixture of ether glyceryl amine, the molar ratio of hypericin and 1,3-diglycidyl ether glyceryl amine is 0.01-1; the heating temperature is 30-50° C., and the stirring time is 30 min.
[0043] Step 2,...
Embodiment 1
[0054] (1) 0.12mmol of 1,3-diglycidyl ether glycerylamine is added to 0.5mL of Tris-HCl buffer solution, and under stirring, the concentration is added to the DMSO dispersion of 0.01mol / L hypericin, Control the reaction temperature to 40° C., make the molar ratio of hypericin and 1,3-diglycidyl ether glyceramine 0.02, and obtain the first dispersion liquid.
[0055] (2) SiO with a concentration of 2mmol / L 2 The PBS dispersion of nanoparticles was heated to 50°C, and the first dispersion was added under stirring to make hypericin and SiO 2 The mass ratio of nanoparticles was 1:1000, and the reaction was carried out at 40°C for 48h. After centrifugal purification, the precipitate was redispersed in deionized water to obtain hypericin-coupled SiO 2 nanoparticle dispersion.
[0056] (3) Disperse 0.1 g of the first compound in 3 mL of dimethylformamide to obtain a second dispersion. Hemoglobin was dispersed in 8 mL of Tris-HCl buffer at 60° C. to obtain a third dispersion. Und...
Embodiment 2
[0059] (1) 0.12mmol of 1,3-diglycidyl ether glycerylamine is added to 0.5mL of Tris-HCl buffer solution, and under stirring, the concentration is added to the DMSO dispersion of 0.01mol / L hypericin, Control the reaction temperature to 40° C., make the molar ratio of hypericin and 1,3-diglycidyl ether glyceramine 0.02, and obtain the first dispersion liquid.
[0060] (2) SiO with a concentration of 2mmol / L 2 The PBS dispersion of nanoparticles was heated to 50°C, and the first dispersion was added under stirring to make hypericin and SiO 2 The mass ratio of nanoparticles was 1:1500, and the reaction was carried out at 40°C for 48h. After centrifugal purification, the precipitate was redispersed in deionized water to obtain hypericin-coupled SiO 2 nanoparticle dispersion.
[0061] (3) Disperse 0.1 g of the first compound in 3 mL of dimethylformamide, stir and heat to 60° C., add 0.01 g of potassium hydride, and react for 1 h to obtain the second dispersion. Hemoglobin was di...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More