Swab nucleic acid sample releaser and application thereof
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A swab nucleic acid and release agent technology, which is applied in the field of swab nucleic acid sample release agents, can solve the problems of increasing the time from collecting samples to the results, operator and laboratory pollution, and complicated operation process, achieving low cost and pollution Less risk, less pollution effect
Active Publication Date: 2021-01-05
DAAN GENE CO LTD
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[0005] In short, the current nucleic acid extraction of swab samples has at least the following disadvantages: (1) high-temperature inactivation of samples is required, which poses a risk of contamination to operators and laboratories, and the amount of nucleic acid will be lost; Additional nucleic acid extracti
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Embodiment 1
[0042] A swab nucleic acid sample release agent, comprising the following components:
[0043] (1) Metal ion chelating agent: 5mol / L EDTA;
[0044] (2) Buffer: pH8.5, 50mmol / L Tris buffer;
[0045] (3) Polar organic solvent: 25% DMSO;
[0046] (4) Surfactant: 2% Triton X-100;
[0047] (5) Nuclease inhibitor: 0.3mol / L guanidine hydrochloride.
[0048] 2. The method of using the above sample release agent is as follows: put the collected nasopharyngeal swab into the sample collection tube containing the sample release agent, and at room temperature, manually mix the sampling tube containing the sample upside down for 30 seconds, Let it stand for 15 minutes, turn it upside down again for 30 seconds, and after instantaneous centrifugation, the sample nucleic acid is released into the sample release agent, and the supernatant is the released nucleic acid solution.
[0049] The obtained nucleic acid solution can be directly pipetted into the PCR reaction solution, and then ampli...
Embodiment 2
[0051] A swab nucleic acid sample release agent, comprising the following components:
[0052] (1) Metal ion chelating agent: 10mol / L EGTA;
[0053] (2) Buffer: pH8, 60mmol / L sodium citrate buffer;
[0054] (3) Polar organic solvent: 30% DMSO;
[0055] (4) Surfactant: 5% NP-40;
[0056] (5) Nuclease inhibitor: 0.5mol / L guanidine isothiocyanate.
Embodiment 3
[0058] A swab nucleic acid sample release agent, comprising the following components:
[0059] (1) metal ion chelating agent: 1mol / L citric acid;
[0063] (5) Nuclease inhibitor: 1mol / L 4,4'-carbonylbis(2-(1-naphthylamide)benzoic acid).
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Abstract
The invention discloses a swab nucleic acid sample releaser and application thereof. The sample releaser comprises 1-20mol/L of metal ion chelating agent, 5-80mmol/L of buffer solution, 5%-40% (v/v) of polar organic solvent, 0.2%-10% (v/v) of surfactant and 0.1-1mol/L of nuclease inhibitor and has a pH value of 6-9. The sample releaser can integrate three steps, i.e., sampling preservation, deactivation and nucleic acid extraction into one step, and a sampled sample can be directly placed into a sample-releaser-containing sampling tube for preservation and transportation; and when in use, thesampling tube is mixed uniformly through up-down reversal, normal-temperature standing is carried out for 15 minutes after the sample and the sample releaser are uniformly mixed thoroughly, and then,nucleic acid of the sample can be released while deactivating the sample. Heating and mid-course uncapping are not required in a whole operating process from sampling to PCR detection, pollution to experiment operating personnel and laboratories is avoided effectively, and the operation is extremely simple; and in case of sudden infectious disease events, the sample releaser has the obvious advantages of rapidness, safety, stability, high efficiency, low cost and the like.
Description
technical field [0001] The invention belongs to the technical field of pathogen molecular detection. More specifically, it relates to a swab nucleic acid sample release agent with simple operation and fast release and its application. Background technique [0002] Nucleic acid molecular detection has incomparable advantages in the detection and screening of pathogens and is widely used. For example, in the recent outbreak of the new coronavirus, nucleic acid testing has played a key role in the screening and testing of infected and asymptomatic people, which is of great significance to the control of the epidemic. At present, swab samples are mainly used in the detection of pathogens such as viruses and the screening of genetic diseases, including nasal swabs, throat swabs, oral swabs, anal swabs, etc. Convenient and widely used. However, the amount of cells collected by swabs is very limited, and the conditions for the growth and storage of cells are extremely harsh. Aft...
Claims
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Application Information
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