Application of polyketide sorbicillinoids as water-soluble photosensitizer
The technology of a water-soluble photosensitizer and compound is applied in the biological field to achieve the effects of simple preparation method, good water solubility and stability, and good bactericidal effect
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Embodiment 1
[0018] The preparation of Sorbicillinoids can refer to patent CN109486688A for details, including the following steps:
[0019] (1) Inoculate the spores of Trichoderma reesei ZC121 in Sabouraud's dextrose medium (SDB), and activate and cultivate at 28°C for 2 days;
[0020] (2) Inoculate activated Trichoderma reesei mycelia in TMM medium containing glucose, and ferment and cultivate for 5 days at 28° C.;
[0021] (3) separating mycelium and fermentation broth by high-speed centrifugation to obtain a fermentation broth supernatant containing inorganic salts, sorbicillinoids, etc.;
[0022] (4) drying the supernatant of the fermentation broth, dissolving it in methanol, and filtering out the inorganic salt insoluble in methanol;
[0023] (5) Evaporate the filtrate to obtain sorbicillinoids.
Embodiment 2
[0025] The sorbicillinoids of test embodiment 1 is to the antibacterial ability of Staphylococcus aureus, and method is as follows:
[0026] Select Staphylococcus aureus cultured overnight, centrifuge and dilute with normal saline until the absorbance of the bacteria at 600nm is about 0.5, at this time the number of bacteria is about 1×10 8 CFU / mL. After incubating Staphylococcus aureus with different concentrations of sorbicillinoids (0, 50, 100, 200, 250, and 300 μg / mL) for 30 minutes, UV rays (2mW / cm 2 ) for 25 minutes, and at the same time, under the same conditions, no ultraviolet light was added as a control. Spread the bacterial solution evenly on the LB agar plate and place it for 12 hours. See the experimental results figure 1 , the experimental results show that 300μg / mL sorbicillinoids can kill 99.5% of Staphylococcus aureus.
Embodiment 3
[0028] The antibacterial ability of the sorbicillinoids of Example 1 to Bacillus subtilis was tested in the same way as in Example 2. See the experimental results figure 2 , the experimental results show that 300μg / mL sorbicillinoids can kill 98.9% of Bacillus subtilis.
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