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Electrochemiluminescence enzyme-linked immunosensor and preparation method and application thereof

An enzyme-linked immunoassay and sensor technology, applied in the field of immunoassay, to achieve the effect of low detection limit, simple and rapid detection range, and good stability

Active Publication Date: 2021-01-15
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no report on a highly sensitive, adjustable detection range ELISA based on mesoporous silica.

Method used

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  • Electrochemiluminescence enzyme-linked immunosensor and preparation method and application thereof
  • Electrochemiluminescence enzyme-linked immunosensor and preparation method and application thereof
  • Electrochemiluminescence enzyme-linked immunosensor and preparation method and application thereof

Examples

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with the embodiments and accompanying drawings.

[0033] Construction Example of Electrochemiluminescent Enzyme-linked Immunosensor

[0034] refer to figure 1 , construction of electrochemiluminescence enzyme-linked immunosensor

[0035] (1) Cut the custom-made ITO electrode into a size of 3cm×2.5cm, soak it in ethanol solution containing 1M NaOH for 24h, ultrasonically clean it with acetone, ethanol and deionized water for 15min, and dry it with nitrogen;

[0036] (2) Synthesis of SANs (mesoporous silica) on the ITO electrode by the solution growth method: First, weigh 0.16g CTAB, dissolve it in a mixed solution of 70mL deionized water and 30mL absolute ethanol, stir until clear, and try to avoid Bubbles are generated; use a micropipette to add 10 μL concentrated ammonia water (concentration 30wt.%) and 80 μL TEOS in sequence, and stir for 10 minutes; transfer the solution into the staining box; fin...

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Abstract

The invention relates to an electrochemiluminescence enzyme-linked immunosensor and application thereof, the electrochemiluminescence enzyme-linked immunosensor is prepared by the following method: firstly, synthesizing mesoporous silica on an ITO electrode, then attaching the mesoporous silica electrode to a paper cover plate, then allowing a biotin labeled antibody and an alkaline phosphatase labeled antibody to react with an antigen to form an antigen-antibody complex; adding avidin magnetic beads to separate the antigen-antibody complex, finally adding a substrate disodium phenylphosphateto react, and detecting the generated phenol, so that the content of the to-be-detected substance is in direct proportion to the generated phenol, and theelectrochemiluminescence enzyme-linked immunosensor is constructed The electrochemical luminescence enzyme-linked immunosensor can be applied to detection of different clinical biomarkers with different concentrations, can simply, conveniently and rapidly adjust the detection range, has a lower detection limit and a wider detection range, and has a wide application prospect in the field of simultaneous detection of multiple components with large content differences.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to an electrochemiluminescent enzyme-linked immunosensor with an adjustable detection range, a preparation method and application thereof. Background technique [0002] Clinical biomarkers are of great significance in disease diagnosis, treatment, condition detection, prognosis judgment and efficacy evaluation. The combined use of multiple clinical biomarkers can provide reliable information for clinical diagnosis and treatment. However, the abundance of clinical biomarkers ranges widely from pg / mL to μg / mL levels. [0003] At present, many detection methods have been applied to the detection of clinical biomarkers, including enzyme-linked immunoassay, immunochemiluminescent assay, immunocolloidal gold, and fluorescent immunoassay. These methods have the disadvantages of high cost, complex instrument structure, high technical requirements, long detection time, and narrow d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N21/76G01N33/543
CPCG01N27/3275G01N21/76G01N33/5438
Inventor 肖依杨小平陈苏华张冉
Owner HUNAN NORMAL UNIVERSITY
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