Processing method of leisure food taking cultivated meat as raw material
A technology of snack food and processing method, applied in the direction of cell dissociation method, biochemical equipment and method, food science, etc., can solve the problems of limited product quantity, less research, insufficient, etc., achieve convenient batch operation and reduce cell death , the effect of improving efficiency
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[0064] 1. Extraction of pig cells
[0065] Piglets were treated with CO 2 Killed by gas, soaked and disinfected with 75% alcohol, dissected the skeletal muscle of the thigh, tore off the fascia, cut the skeletal muscle tissue into tissue pieces of about 0.5g / piece, and then cut it into minced shape with ophthalmic scissors , the particle size is about 1mm 3 ,
[0066] After washing once with PBS, add 2.5mL / g trypsin solution for digestion, digest at 37°C for 25min, add 5mL culture medium, pipette repeatedly, and filter with a filter, centrifuge the filtrate at 800rpm for 5min, and suck off the supernatant , the lower layer of the pellet is the target muscle satellite cells. Place the pellet in high-glucose DMEM proliferation medium containing 20% fetal bovine serum, and let it stand at 37°C, 5% CO 2 Conditioned sterile culture in a cell culture incubator.
[0067] Culture for 2-3 days, pour out the supernatant and lumps, wash with DBPS, then add 1mL trypsin (0.25%) to di...
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