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Processing method of leisure food taking cultivated meat as raw material

A technology of snack food and processing method, applied in the direction of cell dissociation method, biochemical equipment and method, food science, etc., can solve the problems of limited product quantity, less research, insufficient, etc., achieve convenient batch operation and reduce cell death , the effect of improving efficiency

Pending Publication Date: 2021-01-22
CHINA MEAT RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) There are relatively few and insufficient researches on the direction of biologically cultivated meat at home and abroad. At present, there are few relatively mature products, and there is also a lack of patents on the food of domestically cultivated meat;
[0007] (2) The current biological cultured meat production process is carried out in a sterile environment, using a large amount of culture medium and serum, the production cost is relatively high, and the amount of product obtained in industrial production is relatively limited, so the amount of product and product form are also very limited
[0008] The difficulty in solving the above problems and defects is: to collect cultivated meat raw materials for food, high moisture content, white color, tasteless, need to be properly seasoned and toned, in order to produce products that meet current consumer needs
Moreover, the raw material production cost of cultivated meat is high, and the need to maximize the use is also a difficulty of the project

Method used

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  • Processing method of leisure food taking cultivated meat as raw material
  • Processing method of leisure food taking cultivated meat as raw material
  • Processing method of leisure food taking cultivated meat as raw material

Examples

Experimental program
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Embodiment example

[0064] 1. Extraction of pig cells

[0065] Piglets were treated with CO 2 Killed by gas, soaked and disinfected with 75% alcohol, dissected the skeletal muscle of the thigh, tore off the fascia, cut the skeletal muscle tissue into tissue pieces of about 0.5g / piece, and then cut it into minced shape with ophthalmic scissors , the particle size is about 1mm 3 ,

[0066] After washing once with PBS, add 2.5mL / g trypsin solution for digestion, digest at 37°C for 25min, add 5mL culture medium, pipette repeatedly, and filter with a filter, centrifuge the filtrate at 800rpm for 5min, and suck off the supernatant , the lower layer of the pellet is the target muscle satellite cells. Place the pellet in high-glucose DMEM proliferation medium containing 20% ​​fetal bovine serum, and let it stand at 37°C, 5% CO 2 Conditioned sterile culture in a cell culture incubator.

[0067] Culture for 2-3 days, pour out the supernatant and lumps, wash with DBPS, then add 1mL trypsin (0.25%) to di...

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Abstract

The invention belongs to the technical field of animal cell culture and meat product processing, and discloses a processing method of a leisure food taking cultivated meat as a raw material. The processing method comprises the following steps of: collecting muscle tissues by using a sterile surgical instrument, cutting the muscle tissues into minced muscle, and extracting, culturing, differentiating and collecting muscle-derived cells; pretreating the muscle tissues, including pickling, coloring, seasoning and the like; forming and performing hot processing; and cooling a sample, and performing packaging treatment to obtain an instant meat product processed by the cultivated meat. The processing method disclosed by the invention improves the cell cultivation efficiency, reduces cell deathand obtains effective cells to the maximum extent; by using a cell factory, the cell collection efficiency is 5-20 times higher than that of a normal culture dish, the cell collection is accelerated,the cell cultivation efficiency is improved, and a large foundation is laid for subsequent food processing; and by adding multiple auxiliary materials and additives and performing freeze forming, batch operation is facilitated, and relatively rich product forms can be obtained.

Description

technical field [0001] The invention belongs to the technical field of animal cell culture and meat product processing, and in particular relates to a processing method of leisure food with cultivated meat as raw material. Background technique [0002] At present: Cultivated meat, also known as cultured meat, clean meat, etc., is a new type of meat food that is collected and processed by controlling the rapid proliferation and directional differentiation of animal cells in vitro. The traditional meat production method is at the cost of consuming a lot of food and water resources and seriously polluting the environment. With economic development, population expansion, and a sharp increase in middle-income groups, the global demand for meat is increasing rapidly. The production of cultivated meat will serve as an efficient, environmentally friendly, and sustainable new meat production method to meet future human meat demands. product supply. The research on cultured meat bel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L13/60A23L13/70A23L5/20A23L13/40C12N5/077
CPCA23L13/60A23L13/428A23L13/72A23L5/20C12N5/0658C12N2509/00C12N2509/10C12N2506/1323
Inventor 王守伟李莹莹李石磊李雨爽刘文婷杨峰曲超
Owner CHINA MEAT RES CENT
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