Fermentation product from complex microbial agent, preparation method and application thereof

A technology of fermented product and compound bacterial agent, which is applied to bacteria, application, lactobacillus and other directions used in food preparation, can solve the problems of low nutritional activity content of feed and microbial contamination, and achieve the improvement of peptide substances, and the process is simple and easy to implement. , the effect of wide application prospects

Active Publication Date: 2021-01-26
四川特驱农牧科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned shortcomings, the present invention solves the defect that the product is easily contaminated by microorganisms in the production process by adding antimicrobial peptides to the combined fermentation animal feed, and also solves the problem of high-cost high-temperature sterilization in the traditional feed processing process. The technical problem of the low nutritional activity content of the feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of compound bacterial agent fermentation product

[0031] (1) Antibacterial stage: 70kg of high-quality 46 soybean meal was selected, mixed with 30kg of dry bran (medium bran), pre-diluted with water to prepare a solid medium, and then 0.1kg of antimicrobial peptides, 10kg of glucose oxidase, 50kg of glucose and water were added. Mix thoroughly, and then ferment at 32°C for 18h to obtain fermented materials for later use;

[0032] (2) Solid state fermentation stage: 1 / 10 times the amount of compound strain and 1 / 60 times the amount of cellulase are added to the fermentation material obtained in the antibacterial stage. Mixed in a ratio of 1:1:1; add water to humidify again, seal and store, and ferment at a constant temperature of 32°C for 60 hours to obtain a composite inoculum fermentation product, and the water content of the composite inoculum fermentation product is 37.5%.

Embodiment 2

[0034] A kind of compound bacterial agent fermentation product

[0035] (1) Antibacterial stage: 60kg of high-quality 46 soybean meal was selected, mixed with 40kg of dry bran (middle bran), pre-diluted with water to prepare a solid medium, and then 0.1kg of antimicrobial peptides, 10kg of glucose oxidase, 20kg of glucose and water were added. Mix thoroughly, and then ferment at 30°C for 24h to obtain fermented materials for later use;

[0036] (2) Solid state fermentation stage: 1 / 10 times the amount of compound strain and 1 / 60 times the amount of cellulase are added to the fermentation material obtained in the antibacterial stage. The mixture was mixed in a ratio of 1:1:1; water was added to humidify again, sealed and stored, and fermented at a constant temperature of 30°C for 72 hours to obtain a composite bacterial agent fermentation product, and the water content of the composite bacterial agent fermentation product was 35%.

Embodiment 3

[0038] A kind of compound bacterial agent fermentation product

[0039] (1) Antibacterial stage: 80kg of high-quality 46 soybean meal was selected, mixed with 20kg of dry bran (middle bran), pre-diluted with water to prepare a solid medium, and then 0.1kg of antimicrobial peptides, 10kg of glucose oxidase, 100kg of glucose and water were added. Mix thoroughly, and then ferment at 35°C for 12h to obtain fermented materials for later use;

[0040](2) Solid state fermentation stage: 1 / 10 times the amount of compound strain and 1 / 60 times the amount of cellulase are added to the fermentation material obtained in the antibacterial stage. The mixture is mixed in a ratio of 1:1:1; water is added to humidify again, then sealed and stored, and fermented at a constant temperature of 30°C for 72 hours to obtain a composite bacterial agent fermentation product, and the water content of the composite bacterial agent fermentation product is 40%.

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Abstract

The invention discloses a fermentation product from complex microbial agent, a preparation method and an application thereof. The preparation method of the fermentation product from complex microbialagent comprises an antibacterial stage and a solid state fermentation stage. The preparation method comprises steps of mixing 46 soybean meal with bran, adding water for pre-dilution to obtain a solidculture medium, then adding antibacterial peptide, glucose oxidase, glucose and water for sufficient mixing, conducting fermentation for 12-24h to obtain a fermentation material for later use; addinglactobacillus plantarum, saccharomyces cerevisiae, bacillus subtilis and cellulase to the fermented material, adding water again for humidification, conducting sealed storage, conducting constant-temperature stationary fermentation at 35 DEG C for 48h-72h to obtain the fermentation product from composite microbial agent. The fermentation product from complex microbial agent prepared by the methodis high in biological activity. The fermentation product from complex microbial agent can be used for preparing a functional animal feed by mixing with puffed corns, thereby improving nutritional effect of the feed, reducing the incidence rate of diseases in animals, and effectively substituting some antibiotics in use. The preparation process is simple, feasible, green and environmentally friendly, and has wider application prospects in the feed industry and the breeding industry.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a composite bacterial agent fermentation product and a preparation method and application thereof. Background technique [0002] In animal husbandry production, the application of antibiotics can improve feed utilization efficiency and economic benefits. However, the misuse of antibiotics in feeds leads to drug resistance and superinfection of pathogenic microorganisms in livestock and poultry; there are drug residues in livestock and poultry products, product quality and safety are seriously affected, and human health is threatened. Therefore, efficient and green alternatives to antibiotics are sought. It has become a research hotspot. Since the 1990s, many researchers at home and abroad have begun to look for alternatives to antibiotics. In addition, the resources of conventional raw materials are in short supply and the utilization is relatively li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/18A23K10/30A23K10/37A23K20/147A23K20/163A23K20/189A23K40/10
CPCA23K10/12A23K10/14A23K10/18A23K10/37A23K20/189A23K20/163A23K20/147A23K10/30A23K40/10A23V2400/169Y02P60/87
Inventor 王玲玲梅绍锋袁敏王贵芬王成刘贵莲钟霞陈柳林玉聪
Owner 四川特驱农牧科技集团有限公司
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