Preparation method of vaterite-containing hydrogel scaffold material

A scaffold material, vaterite technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problem of inability to provide an extracellular matrix environment, and achieve the effect of promoting bone growth, good mechanical properties, and easy process amplification.

Pending Publication Date: 2021-01-29
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the ionically cross-linked gelatin formed by soaking in salt solution can provide sufficient mechanical strength of the target material, the material is easily degraded in vivo and cannot provide a relatively long-term extracellular matrix environment

Method used

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  • Preparation method of vaterite-containing hydrogel scaffold material
  • Preparation method of vaterite-containing hydrogel scaffold material
  • Preparation method of vaterite-containing hydrogel scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Vaterite-rich Novel Hydrogel Scaffolding Materials

[0027] Step 1: Preparation of Vaterite

[0028] Prepare Na at a concentration of 50 mM 2 CO 3 50 mL of the solution, and 0.1 g of casein was added to it to fully dissolve. Under stirring at 600 r / min, the Na 2 CO 3 Transfer the casein / casein solution to 50 mM CaCl 2 The solution was placed in 50 mL of the solution, stirred for 20 min, and the precipitate was collected by centrifugation. Then, it was washed three times with deionized water, and the precipitate was freeze-dried. It was detected by XRD that the obtained precipitate was vaterite. The SEM image ( figure 1 ) shows that vaterite is spherical particles with a diameter of 1–3 μm.

[0029] Step 2: Preparation of new vaterite-rich hydrogel scaffolds

[0030] The gelatin was dissolved in water at 40 °C to prepare a solution of 100 mg / mL. The mass ratio of vaterite to gelatin was 1:10, and 10 mg of vaterite was added to the gelatin ...

Embodiment 2

[0032] Example 2 Preparation of Vaterite-rich Novel Hydrogel Scaffolding Materials

[0033] Step 1: Preparation of Vaterite

[0034] Prepare Na at a concentration of 60 mM 2 CO 3 The solution was 50 mL, and 0.3 g of casein was added to it to fully dissolve. Under stirring at 600 r / min, the Na 2 CO 3 Transfer the casein / casein solution to 60 mM CaCl 2 The solution was stirred for 20 min in 50 mL, the precipitate was collected by centrifugation, washed with deionized water for 3 times, and the precipitate was freeze-dried to obtain vaterite. The results were similar to those obtained in Example 1.

[0035] Step 2: Preparation of new vaterite-rich hydrogel scaffolds

[0036] Dissolve gelatin in 40°C water to prepare a solution of 200 mg / mL, add 15 mg of vaterite to the gelatin solution with a mass ratio of vaterite to gelatin of 1:15, and disperse uniformly under stirring at 600 r / min. The gelatin solution containing vaterite was poured into the mold, aged at 10 °C for 12 ...

Embodiment 3

[0037] Example 3 Preparation of a novel hydrogel scaffold rich in vaterite

[0038] Step 1: Preparation of Vaterite

[0039]Prepare Na at a concentration of 80 mM 2 CO 3 50 mL of the solution, and 0.4 g of casein was added to it to fully dissolve. Under stirring at 600 r / min, the Na 2 CO 3 Transfer the casein / casein solution to 80 mM CaCl 2 The solution was stirred for 20 min in 50 mL, the precipitate was collected by centrifugation, washed with deionized water for 3 times, and the precipitate was freeze-dried to obtain vaterite. The results were similar to those obtained in Example 1.

[0040] Step 2: Preparation of new vaterite-rich hydrogel scaffolds

[0041] The gelatin was dissolved in water at 40°C to prepare a solution of 300 mg / mL. The mass ratio of vaterite to gelatin was 1:20, and 20 mg of vaterite was added to the gelatin solution, and the mixture was uniformly dispersed under stirring at 600 r / min. The gelatin solution containing vaterite was poured into the...

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Abstract

The invention discloses a preparation method of a novel vaterite-containing hydrogel scaffold material. The preparation method comprises the following steps: firstly, synthesizing vaterite from casein, Na2CO3 and CaCl2, then mixing the synthesized vaterite with a gelatin solution according to a certain ratio, respectively carrying out ion crosslinking and chemical crosslinking by virtue of an ammonium sulfate solution and a mixed solution of NHS (N-hydroxysuccinimide) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) in sequence; and carrying out freeze drying to obtain thenovel vaterite-rich hydrogel scaffold material. The hydrogel scaffold has good biocompatibility and mechanical properties, has large porosity and pore diameter, and can provide a very potential degradable biomedical material for enriching and optimizing a tissue defect degradable scaffold.

Description

technical field [0001] The invention relates to a preparation method of a novel hydrogel support material containing vaterite. Background technique [0002] Natural macromolecular polymer hydrogels are soft in texture and rich in water, which can mimic the physiological environment, and also have the advantages of good biocompatibility and allow the transfer of nutrients within the material. In addition, hydrogels with appropriate mechanical strength can simulate and maintain the biological stress environment of tissues and play a crucial role in promoting tissue regeneration. However, the high water content (nearly 90%) of hydrogels is always accompanied by weak mechanical strength, which largely limits the application of this material in bone regeneration. Salt ions not only affect the stability of protein colloids, but also affect their solubility, that is, different concentrations of salt solutions can increase or decrease the solubility of proteins. Based on this theo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/02A61L27/50A61L27/52A61L27/58
CPCA61L27/025A61L27/222A61L27/227A61L27/50A61L27/52A61L27/58A61L2300/412A61L2430/02C08L89/00
Inventor 惠俊峰李博张敏范代娣郑晓燕
Owner NORTHWEST UNIV(CN)
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