Periodontal soft tissue bionic chip constructed on basis of microfluidic technology and application of periodontal soft tissue bionic chip

A microfluidic chip and soft tissue technology is applied in the field of bionic chips to achieve the effect of improving high throughput and improving chip utilization.

Pending Publication Date: 2021-02-02
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Periodontal soft tissue bionic chip constructed on basis of microfluidic technology and application of periodontal soft tissue bionic chip
  • Periodontal soft tissue bionic chip constructed on basis of microfluidic technology and application of periodontal soft tissue bionic chip
  • Periodontal soft tissue bionic chip constructed on basis of microfluidic technology and application of periodontal soft tissue bionic chip

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Embodiment 1

[0033] like figure 1As shown, a periodontal soft tissue bionic chip based on microfluidic chip technology, a periodontal soft tissue bionic chip based on microfluidic chip technology, including an upper substrate 1, a porous membrane 2 and a lower layer from top to bottom Substrate 3, said upper substrate 1 is provided with 2 parallel outlet microchannels a 1-2, upper substrate 1 is also provided with inlet microchannel a1-1, inlet microchannel a 1-1 and outlet The microchannel a 1-2 vertically forms a "soil"-shaped structure, wherein the four ends of the liquid outlet microchannel a 1-2 are each provided with a liquid outlet a 1-3, and the liquid inlet microchannel a 1-1 is A first liquid inlet 1-4 is provided at the end point that does not intersect with the outlet microchannel a 1-2, and two chambers a 1-6 are respectively provided on the two outlet microchannels a 1-2, and The two chambers a 1-6 on each liquid outlet microchannel a 1-2 are distributed on both sides with t...

Embodiment 2

[0043] The present invention selects the primary cell human gingival epithelial cells (HGE) (primary culture of human gingival epithelial cells: after the patients with periodontitis have written informed consent, cut off the "collar" gingival tissue (collar: That is, the gingival tissue covering the neck of the tooth), immediately put it into the D-Hanks solution containing 3 to 4 times double antibody at 37°C for about 1h. Put the gingival tissue in 2.5g / LDispase II enzyme at 4°C overnight, and the epithelial tissue and After the connective tissue is separated and shredded, place the separated epithelial tissue in 2 mL of 0.025% EDTA-free trypsin, shake gently and mix well, let it stand for digestion for 10 minutes, add DMEM medium containing 10% serum to stop the digestion, 1000r / min Centrifuge for 5 minutes and discard the supernatant, retain the tissue pieces, add an appropriate amount of HuMedia-KG2 "Kurabo, Osaka, Japan, KK-2150S", a special medium for epithelia with sup...

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Abstract

The invention discloses a periodontal soft tissue bionic chip constructed on the basis of a microfluidic chip technology and application of the periodontal soft tissue bionic chip, and belongs to thefield of microfluidic chips. The microfluidic chip mainly consists of eight upper and lower cell culture tanks, a sample inlet/outlet and a microfluidic liquid channel. Multi-chamber design can realize high throughput of cells and improve the utilization efficiency of the chip. A method mainly comprises the following steps: 1) pouring a chip matrix adhesive; 2) performing chip cell inoculation andculture; and 3) monitoring the vascular barrier function of the periodontal soft tissue of the chip. The microfluidic chip is provided with eight cavities, upper fluid and lower fluid can be subjected to substance exchange for four times on a porous membrane for separating the eight cavities, so that the cell migration process and the barrier damage process of a gingival epithelial barrier in theperiodontal soft tissue can be simulated with high simulation degree, and besides, the construction of a periodontal soft tissue interface can also be simulated. The chip is applied to researches oncell co-culture, cell barrier construction, cell migration and the like.

Description

technical field [0001] The invention relates to a bionic chip, in particular to a periodontal soft tissue bionic chip constructed based on microfluidic chip technology and an application thereof. Background technique [0002] Periodontal disease is a bacterial biofilm-associated inflammatory disease caused by the transfer of local microorganisms, which causes the progressive destruction of periodontal tissue and eventually tooth loss. Since periodontal disease is involved in many systemic diseases, such as diabetes, cardiovascular disease, aspiration pneumonia, dementia, etc., the prevention of periodontal disease is very important to promote human health. However, periodontitis may increase the overall risk of systemic disease through systemic inflammation and immune responses induced by microorganisms and their products in subgingival plaque biofilms. The combined epithelial barrier in periodontal soft tissue is the first line of defense against foreign invaders, and its ...

Claims

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Application Information

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IPC IPC(8): C12M3/06C12N5/071B01L3/00
CPCC12M21/08C12M23/16C12M25/14C12M29/04C12M35/08C12N5/069C12N5/0625B01L3/5027C12N2502/09C12N2502/28C12N2533/90Y02A50/30
Inventor 刘慧颖马国武毛红菊靳来娣安凡罗勇林炳承
Owner DALIAN MEDICAL UNIVERSITY
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