Rapid forming method of electrode biological membrane and microbial electrochemical sensor
A molding method and biofilm technology, applied in biochemical equipment and methods, material electrochemical variables, microbial measurement/inspection, etc., can solve the problems of slow start-up reproducibility, etc., and achieve excellent sensitivity, simple process, and signal Highly reproducible effect
Inactive Publication Date: 2021-02-02
TSINGHUA UNIV
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Problems solved by technology
However, the spontaneously formed electroactive biofilm will be affected by many environmental factors, so the rapid prototyping of electrode biofilm sensitive components is an effective way to solve the problem of slow startup, reproducibility and promote the standardization process
Method used
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Embodiment 1
[0022] In embodiment 1, selection adopts the substratum (LB solution) that has included 5g / L yeast powder, 10g / L tryptone and 10g / L NaCl to cultivate, enrich Shewanella MR-1, after reaching logarithmic phase, extract It was collected by centrifugation, and the collected strains were rinsed with phosphate buffer to remove the residual medium and then constant volume to obtain a concentration of 10 7 Concentrated strains of CFU / mL. A sodium alginate solution with a concentration of 20g / L was prepared, and it was fully mixed with suspended bacteria at a volume ratio of 1:1 to obtain a composite preparation of electroactive bacteria. The prepared composite formulation was spin-coated on the current collector by controlling the speed and time of the homogenizer. In this embodiment, the current collector is selected as a planar ITO electrode (conductive glass). In order to make the spin coating controllable, a mold is also attached to the conductive glass. The mold is a silica gel ...
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Abstract
The invention discloses a rapid forming method of an electrode biological membrane and a microbial electrochemical sensor applying the rapid forming method. The preparation method comprises the following steps: uniformly mixing enriched suspended electroactive bacteria with a proper amount of an adhesive solution to prepare an electroactive bacteria composite preparation, and then uniformly coating a current collector with the electroactive bacteria composite preparation by a spin-coating method, so that the current collector is rapidly formed into an electrode biofilm; pressing and covering the electrode biological membrane and an electrode material at least comprising one current collector to obtain a working electrode, and connecting the working electrode, a counter electrode and a potentiostat to form the microbial electrochemical sensor for early warning of water quality pollution. The method has the advantages of fast biofilm formation, sensitive water quality early warning and the like.
Description
technical field [0001] The invention relates to an electrode biofilm rapid forming method and a microbial electrochemical sensor, belonging to the technical field of microbial electrochemical sensors. Background technique [0002] Water pollution is a major problem faced by countries all over the world, and water quality early warning is an effective way to control this problem. Water quality early warning refers to the comprehensive monitoring of polluted water bodies through physical, chemical and biochemical methods, making a preliminary judgment on toxicity and issuing warnings to users, providing a basis for downstream standardized testing, thereby ensuring human health and ecological environment safety. Traditional physical and chemical methods can only judge the concentration of pollutants, and cannot directly reflect the biological effects. Further judgments need to be made indirectly through relevant standards. Microbial electrochemical sensors use electroactive bi...
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IPC IPC(8): G01N27/26G01N27/327C12Q1/02
CPCC12Q1/025G01N27/26G01N27/3275
Inventor 梁鹏戚祥黄霞
Owner TSINGHUA UNIV