Composite microbial agent for inhibiting pathogenic bacteria as well as preparation method and application of composite microbial agent

A technology of compound microbial inoculants and pathogenic bacteria, applied in the field of biological bacteria, can solve problems such as poor effect and singleness, and achieve the effects of improving killing effect, promoting decomposition, and good preventive and therapeutic effects.

Pending Publication Date: 2021-02-05
中诚国联(河南)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods to prevent and treat Fusarium wilt. One of the most promising methods is to use biological agents for treatment, but the existing biological agents generally use a single strain, and the effect of preventing and treating Fusarium wilt is relatively poor.

Method used

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  • Composite microbial agent for inhibiting pathogenic bacteria as well as preparation method and application of composite microbial agent
  • Composite microbial agent for inhibiting pathogenic bacteria as well as preparation method and application of composite microbial agent
  • Composite microbial agent for inhibiting pathogenic bacteria as well as preparation method and application of composite microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) 20 parts by weight of Bacillus subtilis, 15 parts of Streptomyces jingyang, 15 parts of Pseudomonas fluorescens and 2 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.

[0030] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.

Embodiment 2

[0032] (1) 25 parts by weight of Bacillus subtilis, 20 parts of Streptomyces jingyang, 20 parts of Pseudomonas fluorescens and 4 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.

[0033] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.

Embodiment 3

[0035] (1) 30 parts by weight of Bacillus subtilis, 25 parts of Streptomyces jingyang, 25 parts of Pseudomonas fluorescens and 6 parts of saccharomyces were respectively put into the corresponding basal medium for activation and expansion culture for 2 days.

[0036] (2) Put Bacillus subtilis, Streptomyces jingyang, Pseudomonas fluorescens and saccharomyces into the fermentation tank after activation and expansion, add 2.3×10 4 Part of water, aeration and stirring culture, ventilation volume 1m 3 / h, the stirring speed is 140rpm, the fermentation temperature is 32°C, and the fermentation is carried out for 3 days to obtain the composite microbial bacterial agent.

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Abstract

The invention is applicable to the technical field of biological bacteria, and provides a composite microbial agent for inhibiting pathogenic bacteria. The composite microbial agent comprises the following components: bacillus subtilis, streptomyces jingyangensis, pseudomonas fluorescens and saccharomycetes. Preferably, the composite microbial agent comprises the components in parts by weight of 30 parts of the bacillus subtilis, 25 parts of the streptomyces jingyangensis, 25 parts of the pseudomonas fluorescens and 6 parts of the saccharomycetes. The composite microbial agent disclosed by theinvention has a good treatment effect on fusarium wilt of plants, also has a good treatment effect on fusarium wilt of yellow flowers, potatoes and tomatoes, and has the characteristic of wide application. Through combined use of the bacillus subtilis, the streptomyces jingyangensis and the pseudomonas fluorescens, the treatment effect on the fusarium wilt of plants is remarkably improved, and asynergistic effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological bacteria, and in particular relates to a compound microbial bacterial agent for inhibiting pathogenic bacteria and its preparation method and application. Background technique [0002] In order to obtain higher yields, humans have continuously used large amounts of chemical fertilizers and pesticides in farmland. The lack of organic matter in the soil in farmland has greatly reduced the types and quantities of microorganisms, including some beneficial microorganisms that can inhibit soil-borne diseases, such as actinomycetes and Trichoderma, which lead to the increasing rampant of fusarium wilt and root rot. [0003] Fusarium wilt is also called blight. A sudden onset of a plant disease caused by fungi or bacteria, with symptoms including severe spotting, wilting, or death of leaves, flowers, fruit, stems, or the entire plant. Rapidly growing young tissues are often attacked. Most commercial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A01N63/32A01N63/28A01N63/27A01N63/22A01P3/00C12R1/125C12R1/465C12R1/39
CPCC12N1/20C12N1/16A01N63/32A01N63/28A01N63/27A01N63/22
Inventor 祝春华朱永杰张君怡孙辉
Owner 中诚国联(河南)生物科技有限公司
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