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Lactobacillus freeze-drying protective agent as well as preparation method and application thereof

A technology of freeze-drying protective agent and lactic acid bacteria, which is applied in the field of microorganisms, can solve the problems of lack of scientific basis for protective agents, and the actual effect of lactic acid bacteria is not very ideal, so as to prevent damage from water crystal formation, inhibit the formation of free radicals, and prevent ice crystal damage Effect

Inactive Publication Date: 2021-02-09
SHENYANG RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of scientific basis in the selection and proportion of protective agents, the actual effect of lactic acid bacteria after freeze-drying is not very ideal, and there is still a lot of room for improvement.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dissolve 8g of maltose, 4g of polyethylene glycol, and 6g of bovine serum albumin in 50mL of distilled water to prepare protective agent mother solution A, sterilize at 115°C for 20min, and cool; weigh 0.5g of glutathione and 0.1g of cobalamin Dissolve in 50mL sterile water to prepare protective agent mother solution B, which is sterilized by passing the mother solution B through a 0.22μm sterile filter; then completely mix the mother solution A and the mother solution B to obtain the protective agent solution.

[0024] Inoculate the seed solution of Lactobacillus brucei into 1L of MRS medium at a volume ratio of 2%, culture it for anaerobic fermentation at 37°C for 24 hours, centrifuge the fermentation liquid at 4000rpm for 30min, collect the sludge of Lactobacillus brucei, Add the above protective agent at a ratio of 1:5, mix evenly, pre-freeze at -20°C for 8 hours, and then freeze-dry at -80°C for 15 hours under a vacuum of 3-10 Pa to obtain freeze-dried Lactobacillus...

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PUM

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Abstract

The invention discloses a lactobacillus freeze-drying protective agent as well as a preparation method and application thereof. The lactobacillus freeze-drying protective agent comprises 7%-10% of maltose, 3%-6% of polyethylene glycol, 4%-8% of bovine serum albumin, 0.3%-0.8% of glutathione and 0.05%-0.2% of cobalamin. Compared with the prior art, the protective agent provided by the invention caneffectively improve the survival rate (the survival rate is greater than 96%) and the effective viable count (the viable count of the bacterial powder is greater than 10<11>cfu / g) of lactobacillus after vacuum freeze drying, and prolongs the shelf life of the bacterial powder (the viable count is greater than 10<11>cfu / g and the loss rate is less than 10% after the bacterial powder is stored at room temperature).

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a lactic acid bacteria freeze-drying protectant and its preparation method and application. Background technique [0002] Lactic acid bacteria are widely used in food, agriculture, animal husbandry, medicine and other fields. At present, lactic acid bacteria products are generally prepared by freeze-drying and other methods. Adding an appropriate protective agent before freeze-drying can reduce the damage to cells during the freeze-drying process and improve the survival of lactic acid bacteria. Fan Zhen et al. optimized the protective agents for Lactobacillus plantarum and Leucobacterium enterica glucan subspecies, and determined that the best protective agent formulations were: 15% skim milk, 6% trehalose, 2% glycerin, and 3% ascorbic acid. and skim milk 15%, trehalose 6%, glycerin 1%, ascorbic acid 2%, after optimization, the freeze-drying survival rates of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/225C12R1/01C12R1/46
CPCC12N1/04C12N1/20
Inventor 李小明杨德玉王远王加友孙丽娟赵彭年
Owner SHENYANG RES INST OF CHEM IND
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