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Simultaneous detection of 8 active ingredients in Xiaoer Magan Granules by hplc method

A technology of Pediatric Magan and active ingredients, applied in the field of HPLC method, can solve problems such as difficulty in comprehensively controlling the quality of traditional Chinese medicine preparations, and achieve the effects of improving peak shape and resolution, easy operation and excellent application effect.

Active Publication Date: 2022-08-05
HUBEI WUDANG JINDING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the content determination of active ingredients is one of the key contents in the quality standards of traditional Chinese medicine. For compound preparations, it is difficult to be objective, comprehensive and sensitive only for a single component or a few components in the preparation. It is difficult to comprehensively control the quality of traditional Chinese medicine preparations

Method used

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  • Simultaneous detection of 8 active ingredients in Xiaoer Magan Granules by hplc method
  • Simultaneous detection of 8 active ingredients in Xiaoer Magan Granules by hplc method
  • Simultaneous detection of 8 active ingredients in Xiaoer Magan Granules by hplc method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 The HPLC method of simultaneously detecting 8 kinds of active ingredients in Xiaoer Magan Granules

[0057] Specific steps are as follows:

[0058]S1. Precisely weigh 0.5g of the sample of Xiaoer Magan granules to be tested. After grinding, place it in a conical flask with a sealed stopper, add 20ml of methanol-diethyl ether mixed solution with a volume ratio of 2.5:1, and weigh it accurately after sealing. After refluxing at 80°C for 30min, place it to cool, use methanol-diethyl ether mixed solution with a volume ratio of 2.5:1 to make up for weight loss, shake well, filter with a 0.45μm microporous membrane, and take the filtrate to obtain the test solution;

[0059] S2. Add 10 μL of trifluoroacetic acid and 70 μL of 4.5 mg / L 3-pyridylmethoxyamine / ethanol solution to the obtained test solution, react at 30° C. for 25 min, and inject into the high performance liquid chromatograph , the chromatogram was obtained after the HPLC method was tested;

[0060] ...

Embodiment 2

[0071] Embodiment 2 The HPLC method of simultaneously detecting 8 kinds of active ingredients in Xiaoer Magan Granules

[0072] The steps of the HPLC method for detecting 8 active ingredients in Xiaoer Magan Granules provided in this example are the same as those in Example 1, except that:

[0073] The mobile phase gradient elution parameters are:

[0074] .

[0075] Compared with Example 1, the separation degree of active ingredient ephedrine hydrochloride and pseudoephedrine hydrochloride, baicalin and rosmarinic acid in Example 2 decreased by 18.7% and 22.4% respectively; in addition, its sensitivity (according to the minimum detection limit of quantification) decreased by 4.2-7.4%.

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Abstract

The invention provides an HPLC method for simultaneously detecting 8 active ingredients in Xiaoer Magan granules, the 8 active ingredients include ephedrine hydrochloride, pseudoephedrine hydrochloride, amygdalin, chlorogenic acid, baicalin, liquiritin, glycyrrhizic acid and rosemary The steps include: measuring a sample of Xiaoer Magan granules to be tested, adding alcohol-ether mixed solution and extracting to obtain a test solution; injecting it into a high performance liquid chromatograph, and testing by HPLC method to obtain a chromatogram; The contents of 8 active ingredients in Xiaoer Magan Granules were calculated by standard method. The present invention can improve the sensitivity by using 3-pyridyl methoxyamine derivatization reagent for derivatization treatment, and can accurately and effectively quantitatively detect low-abundance active components; by optimizing the mobile phase and gradient elution mode, the chromatographic peak difference is improved. The detection method has high sensitivity; the method has fast detection speed, high sensitivity, high accuracy and good repeatability.

Description

technical field [0001] The invention relates to the technical field of detection of active pharmaceutical ingredients, more particularly, to an HPLC method for simultaneously detecting 8 active ingredients in Xiaoer Magan Granules. Background technique [0002] Pediatric cough is a common clinical disease, but it is rarely cured quickly. At present, neither oral chemical drugs nor infusions can cure coughs completely and quickly; in comparison, traditional Chinese medicine preparations have few side effects, even if the medication cycle is slightly longer (7-10 days), no side effects will appear, so the advantages of traditional Chinese medicine are significantly reflected. . [0003] Xiaoer Magan Granules are derived from the classic prescriptions in Treatise on Febrile Diseases and Miscellaneous Diseases written by the medical sage Zhang Zhongjing. It has the functions of relieving asthma, relieving cough and removing phlegm from the throat, and is used for the treatment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 黄正军何德苟肖作武王世才陈昊
Owner HUBEI WUDANG JINDING PHARMA