Microneedle coated with drug and manufacturing method for same
A manufacturing method and microneedle technology, which can be applied in the direction of drug devices, microneedles, pharmaceutical formulations, etc., can solve the problems of reducing the dip coating rate and exerting little effect, and achieve the effect of improving wrinkles and low skin permeability.
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Embodiment 1
[0084] Example 1: Manufacture of drug-coated microneedles
[0085] 1-1: Purification containing silicon dioxide (SiO 2 ) microneedle
[0086] Spongilla. fragilis Leidy needle-shaped bone pieces (289g) and sulfuric acid (80g) were put into a reaction tank and stirred for 1 hour, then water was added for additional stirring and filtered. Wash the spicules with water, and stir for 1 to 2 hours, then add NaOH and HNO respectively 3 Stirring was performed, and the pH was adjusted to 6 to 8, followed by washing with water. Finally, the spicules were washed with ethanol, filtered and dried.
[0087] 1-2: Modification containing silicon dioxide (SiO 2 ) of the microneedle surface
[0088] In order to introduce sulfhydryl (Sulfhydryl, -SH) reactive groups into purified acicular bone pieces (containing silicon dioxide (SiO 2 ) of the microneedle), carried out the hydrolysis reaction and deoxyalkylation reaction.
[0089] 1-2-1: Surface modification using hydrolysis reaction
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experiment example 1
[0172] Experimental Example 1: Evaluation of Collagen Synthesis-Promoting Ability of Drugs Containing Sulfhydryl (-SH) Reactive Groups
[0173] 1-1: Selection and cultivation of cell lines
[0174] Fibroblasts (CCD-986SK) purchased from the Korean Cell Line Bank were inoculated on the bottom of the culture dish, and then penicillin (100 IU / mL), streptomycin (100 μg / mL), and 10% FBS (fetal bovine serum, Fetal bovine serum) in DMEM (Dulbecco's Modified Eagle's Medium) medium was maintained at 37° C. and cultured in an incubator containing 5% carbon dioxide.
[0175] 1-2: Cytotoxicity test method (CCK assay)
[0176] Divide fibroblasts (CCD-986SK) in 24-well (or 96-well) plate per hole 1.0×10 4 (~5.0×10 4 ), and then cultured for 24 hours under cell culture conditions. The medium was discarded, washed with PBS, and then replaced with a new DMEM medium not containing 10% FBS, and the cells were treated with predetermined concentrations of the test substances (GHK and GHK-TA) a...
experiment example 2
[0184] Experimental Example 2: Evaluation of Collagen Synthesis-Stimulating Function of Drug-Coated Microneedles
[0185] 2-1: Collagen production potential of linker (X) with Sulfhydryl (-SH) reactive group
[0186] Peptides were separated from the microneedles using 0.3 g of drug-coated microneedles (GHK-TA-MN, GHK-MPA-MN, GHK-CS-MN) manufactured in Examples 1-4 and 0.1M GSH (glutathione) 40 μl After 840 minutes, centrifuge the supernatant, then evaluate the potential of promoting intracellular collagen production in the same way as in Experimental Example 1-3, the results are as follows Figure 5 shown.
[0187] Such as Figure 5 As shown, it can be understood that there are differences in the collagen production promoting potential according to the type of linker (X) having a sulfhydryl (Sulfhydryl, -SH) reactive group. It is speculated that this difference is caused by the molecular weight of the linker with Sulfhydryl (-SH) reactive group. For reference, the molecula...
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