Composition for improving intestinal function comprising leuconostoc sp. strain
A technology of Leuconostoc heshii and composition, which is applied in the field of compositions for improving intestinal function, and can solve problems such as lack of treatment methods
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Embodiment 1
[0041] Example 1: Metagenomic Analysis of Fecal Bacteria in Patients with Gut-Related Diseases
[0042] For metagenomic analysis of bacteria in stool from patients with gut-related diseases, stool samples were collected from 38 patients with colon cancer, 64 with ulcerative colitis, and 65 with Crohn's disease. As a control group, feces of 74 normal persons were used. In order to remove bacteria from the stool, first use medical gauze to remove the large particles present in the stool. Then, put the feces from which large particles were removed into a 10mL test tube, and centrifuge at 3500×g for 10 minutes at a temperature of 4°C to separate the pellet and supernatant. solution was added to each new 10mL test tube. Use a 0.22μm filter to remove bacteria and foreign matter on the supernatant, transfer to a centrifugal filter (Centrifugal filter) 50kD, centrifuge at 1,500×g at 4°C for 15 minutes to remove substances smaller than 50kD, and then concentrate to the total The cap...
Embodiment 2
[0049] Embodiment 2: the preparation of the powder of Leuconostoc heshii bacterial strain and culture fluid
[0050] In order to confirm the intestinal or liver function improving ability of the Leuconostoc holzapfelii strain, the Leuconostoc holzapfelii strain Ceb-kc-003 (KCCM11830P) deposited in the Korean Microorganism Collection was obtained, and after adding 3% chlorine cultured in sodium chloride BHI solid medium. Leuconostoc heschii powder was prepared by freeze-drying cultured strains. To prepare a culture solution, 2 kg of glucose, 1.5 kg of whole milk powder, and 0.05 kg of yeast extract were added to 100 L of purified water and incubated at 121°C. After sterilizing at high temperature and pressure for 15 to 30 minutes, cool it to room temperature. Then, 0.2 to 0.4 L of the Leuconostoc hayesi Ceb-kc-003 strain was inoculated in a sterile state, and cultured at a temperature of about 35° C. for 2 to 3 days to prepare a Leuconostoc hayesi culture.
Embodiment 3
[0051] Example 3: Confirmation of intestinal function improvement effect of Leuconostoc hayesi strain
[0052] In order to confirm the intestinal function improvement effect of Leuconostoc heschii strains, 20 volunteers (8 males and 12 females) were recruited. The average age of the volunteers was 32.0±5.4 years old, and the average age of the males was 31.8 years old. ±3.6 years (26 to 37 years), the average age of females was 32.2±6.3 years (21 to 47 years). Will 1×10 6 to 1×10 8 CFU (colony forming unit: colony forming unit) / mL Leuconostoc heschii Ceb-kc-003 strain culture solution was orally administered to the subject twice a day for a total of 4 weeks. In the same way as in Example 1, the volunteers' feces were collected on the day of the clinical trial (before ingestion) and 4 weeks later (after ingestion), and the Acinetobacter and Pseudomonas species contained in the feces were confirmed. Changes in bacteria. show the result in figure 2 and Table 3.
[0053] 【t...
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