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Nano drug-loading system and its application of sex attractant of Spodoptera frugiperda

A technology of Spodoptera frugiperda and nano-drug loading, which is applied in application, attracting pesticides, biocides, etc., can solve problems such as inconsistent attracting effects, inability to guarantee sustained release of sex pheromones, and single preparation method, achieving The trapping effect is enhanced, the slow-release time is improved, and the slow-release effect is good

Active Publication Date: 2021-11-09
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition and ratio of the sex pheromones of fall armyworm are different due to different geographical populations, and the commercialized sex attractants of fall armyworm have different attracting effects
Moreover, the preparation method is single, and the sustained release of sex pheromone cannot be guaranteed. Therefore, the present invention proposes a preparation method, using amphiphilic lipid molecules to load Spodoptera frugiperda sex pheromone and plant volatile linalool nano-loaded drug The construction of the system, the preparation of a sex attractant with sustained slow-release effect for the control of Spodoptera frugiperda

Method used

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  • Nano drug-loading system and its application of sex attractant of Spodoptera frugiperda
  • Nano drug-loading system and its application of sex attractant of Spodoptera frugiperda
  • Nano drug-loading system and its application of sex attractant of Spodoptera frugiperda

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Preparation of sex pheromones

[0020] The three components of sex pheromone (Z)-9-tetradecen-1-ol acetate, (Z)-7-dodecen-1-ol acetate and (Z)-11- The hexadecen-1-ol acetate was prepared according to the mass percentage of 90:0.5:9.5, and the solvent was tetrahydrofuran.

[0021] 2) Construction of a nano-drug delivery system for Spodoptera frugiperda sex attractants

[0022] Take 1 mL of the mixed solution prepared in step 1) (the mass of the three-component mixed solution is 100 mg) and 1 mg of synergist linalool, and mix them with 1 mg of the amphiphilic lipid molecule DSPE-PEG under ultrasound. 5000 Mix, then pour 9 mL of distilled water and continue sonication for 120 s. Then the mixture was stirred at room temperature overnight, and the organic solvent was evaporated to obtain nanoparticles constructed by the nano-drug delivery system.

[0023] 3) Preparation of Spodoptera frugiperda sex attractants

[0024] Take 100 μL of Spodoptera frugiperda nanoparticle...

Embodiment 2

[0026] The specific steps are the same as those in Example 1: the synergist linalool in step 2) is not added in this step, and the others are the same, and the resulting lure is numbered N2.

Embodiment 3

[0028] Specific implementation steps:

[0029] Same as Example 1, step 1) The three different components of sex pheromone are prepared according to the mass percentage of 90:0.5:9.5, the mass of the mixed solution is 100 mg, and it is diluted to 1 mL with n-hexane to obtain a mixed solution of sex pheromone;

[0030] Step 2) Take 10 μL of the sex pheromone mixture in 1) with a pipette, slowly inject it into a polyvinyl chloride capillary, and the lure is ready, numbered N3.

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Abstract

The invention discloses a nanometer drug-carrying system of a sex lure of Spodoptera frugiperda and its application. They are amphiphilic lipid molecule-loaded pheromone and synergist linalool: composed of amphiphilic lipid molecule DSPE-mPEG 5000 Self-assembly of (1,2‑distearoyl‑sn‑glycerol‑3‑phosphoethanolamine‑N‑[methoxy(polyethylene glycol)‑5000]), sex pheromones, and linalool via a cosolvent method , and then adsorbed on a slow-release carrier 0.22 μm polyethersulfone membrane. The sex pheromone is composed of (Z)‑9‑tetradecen‑1‑alcohol acetate, (Z)‑7‑dodecene‑1‑alcohol acetate and (Z)‑11‑deca The mass ratio of hexacene-1-alcohol acetate is 90:0.5:9.5. It also discloses its moth-attracting effect in the field as a fall armyworm sex attractant.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and in particular relates to a nanometer drug-carrying system for Spodoptera frugiperda sex attractants and applications thereof. Background technique [0002] Spodoptera frugiperda ( Spodoptera frugiperda ), belonging to the genus Spodoptera, belonging to the Lepidoptera family Noctuidae, is reported to originate from tropical and subtropical regions of the Americas and is a highly destructive pest. In January 2019, it was found in Yunnan Province, my country, and by May, it had spread and invaded 18 provinces (cities, districts) in China. Up to now, 27 provinces have been found to be invaded and harmed by Spodoptera frugiperda, and they continue to move northward. [0003] Spodoptera frugiperda is a polyphagous insect that can cause damage to a variety of crops, including corn, rice, sorghum, wheat, sugar cane, cotton, and canola. Spodoptera frugiperda larvae have different p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N49/00A01N37/02A01N25/10A01P19/00
CPCA01N25/10A01N37/02A01N49/00
Inventor 陈秀琴邱良妙刘其全何玉仙占志雄
Owner INST OF PLANT PROTECTION FAAS
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