New application of stachyose in the preparation of drugs for preventing and/or treating polycystic ovary syndrome
A polycystic ovary and stachyose technology, applied in the field of stachyose, can solve the problems of dose dependence and large side effects in the human body, and achieve the effects of promoting rapid proliferation, restoring ovulation function, and reducing the number of populations
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Embodiment 1
[0066] The construction of embodiment 1 animal model
[0067] Three-week-old SD rats were divided into three groups: the control group, the PCOS model group, and the stachyose treatment group, with 9-11 rats in each group. The construction method of each group of rat models is as follows:
[0068] (1) Control group (Oil+Saline group): 200 μL of sesame oil was subcutaneously injected for 21 consecutive days, followed by intragastric administration of normal saline for 12 consecutive days.
[0069] (2) PCOS model group (DHEA+Saline group): Subcutaneous injection of dehydroepiandrosterone (DHEA) (Solarbio, D8950) 6 mg / 100 g, 200 μL, continuous injection for 21 days, followed by intragastric administration of normal saline for 12 consecutive days.
[0070] (3) Stachyose treatment group (DHEA+STA group): subcutaneous injection of dehydroepiandrosterone (DHEA) (6mg / 100g, 200μL), after continuous injection for 21 days, stachyose (TCI, S0397 ) (200mg / kg).
[0071] Weighing was perf...
Embodiment 2
[0072] Example 2 The observation of rat estrous cycle, the observation of ovarian histomorphology and the measurement and analysis of serum hormones 1, the observation of rat estrous cycle
[0073] Rats are animals with multiple estrus throughout the year and have a relatively stable sexual cycle, which is divided into: preestrus (P), estrus (E), postestrus (M) and diestrus (D). The vaginal mucosa of each period is Typical changes occur, which stage can be judged according to the cytological characteristics of the vaginal smear. In this embodiment, 3 rats in the control group (Oil+Saline group), PCOS model group (DHEA+Saline group), and stachyose treatment group (DHEA+STA group) three groups of experimental animals were treated at the same time every afternoon. The vaginal cell smears were taken for 10 consecutive days, stained with Wright's staining solution (LEAGENE, 1029A20) to observe the cell morphology, and the experimental results were recorded and counted.
[0074] 2....
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