Tissue culture and rapid propagation method for grape rootstocks

A technology of tissue culture rapid propagation and rootstock, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of time and season restrictions on material collection, inconvenient actual operation, toxic disinfectants, etc., and achieve good disinfection effect , Increase the survival rate of transplanting, the effect of appropriate concentration
CN112438202AInactive Publication Date: 2021-03-05CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-03-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical field of plant tissue culture and seedling raising, in particular to a tissue culture and rapid propagation method for grape rootstocks. The method comprises thefollowing steps of taking dormant branches of grape rootstocks, shearing the dormant branches into single-bud stems, breaking physiological dormancy, carrying out water culture on the single-bud stems until the single-bud stems germinate, and sampling when new shoots are 10-20cm long so as to obtain explants; shearing wounds at the top end and the base part of the explant, and inoculating the explant into an initial culture medium for initial culture; when new shoots grow to 5-6 cm, inoculating the new shoots into a proliferation culture medium for proliferation culture, carrying out subculture once every 40-50 days, and carrying out subculture for 3-4 generations; and selecting tissue culture seedlings for seedling hardening and transplanting. According to the method, the initial culturemedium can obviously induce the stem segments of the grape rootstocks to grow new shoots or cluster buds; and the new shoots or cluster buds subjected to initial culture in the propagation culture medium can be subjected to propagation culture and simultaneously germinate and root to form seedlings through one-step induction, and only one propagation culture medium needs to be prepared, so that the propagation efficiency is improved.
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Description

technical field

[0001] The invention relates to the technical field of plant tissue culture and seedling cultivation, in particular to a tissue culture and rapid propagation method for grape rootstocks. Background technique

[0002] In recent years, my country's grape planting area has greatly increased, and the grape industry has developed rapidly. The traditional self-rooted seedlings are the main cultivation method in my country's grape cultivation, but grafted seedlings are gradually being valued due to their resistance to diseases and insect pests, especially the characteristics of resistance to phylloxera. In grapevine grafts, the shoot portion of the union of the two grafted together is called the scion, while the rootstock provides the root system. In addition to enhancing stress resistance, good rootstocks can also promote the growth of scions and affect the quality and flavor of grapes. Therefore, it is particularly important to obtain virus-free high-quality gra...

Claims

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