Tissue culture and rapid propagation method for grape rootstocks

A technology of tissue culture rapid propagation and rootstock, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of time and season restrictions on material collection, inconvenient actual operation, toxic disinfectants, etc., and achieve good disinfection effect , Increase the survival rate of transplanting, the effect of appropriate concentration

Inactive Publication Date: 2021-03-05
CHINA AGRI UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing grape rootstock tissue culture and rapid propagation technology, explants can only be obtained during the growing season, which is limited by time and season, and the disinfectant used to sterilize the explants is toxic, which is inconvenient for actual operation
And the proliferation medium of the tissue culture seedling is different from the composition of the rooting medium, and the operation is complicated
In terms of domestication and transplantation of tissue culture seedlings, other materials are needed, which is inconvenient to operate and high in application cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and rapid propagation method for grape rootstocks
  • Tissue culture and rapid propagation method for grape rootstocks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Explant collection

[0058] Collect healthy dormant branches of virus-free grape rootstock '101-14', cut the dormant branches into single-bud stem segments, the upper end of the bud is flat, 2-3 cm away from the bud, and the lower end is oblique, 3-4 cm away from the bud. The physiological dormancy was broken after being treated in a constant temperature water bath at 45°C for 90 minutes, and the single-bud stem segment was floated in clean water with a foam board for cultivation. The culture environment is light intensity 30~40μmol·m -2 ·s -1 (or 2000Lx~3000Lx), the light time is 14~16h, and the temperature is 25±2℃. Sampling was carried out when the length of new shoots reached 10-20 cm, and stem segments cut to 5 cm were used as explant materials.

[0059] (2) Surface disinfection of explants

[0060] Put the stem segment into the container and soak it with detergent for 5-10 minutes, then rinse it with running water for 2-3 hours, and finally rinse it with d...

Embodiment 2

[0076] Collect the dormant branches of the virus-free grape rootstock 'SO4', treat the dormant branches in hydroponics, disinfect the surface of the explants, inoculate the explants and initiate proliferation. The culture conditions are the same as in Example 1. In the proliferation culture, when the medium condition is WPM+IBA0.2mg / L+sucrose 30g / L+agar 6.5~7g / L, the second-generation proliferation coefficient of 'SO4' is 2.7, and sprouts and roots at the same time, the third-generation multiplication coefficient is 2.8, and the root system is developed And the density of the main root is relatively high, and the leaves and roots are not browned; the steps of hydroponic seedling hardening and domestication transplanting are the same as those in Example 1, and the survival rate of 'SO4' is 94.5% in hydroponic cultivation and 92.3% in transplanting.

Embodiment 3

[0080] The dormant branches of the virus-free grape rootstock '110R' were collected, the dormant branches were treated in hydroponics, the surface disinfection of the explants, the inoculation of the explants and the culture conditions for initial proliferation were the same as in Example 1. In the proliferation culture, when the medium condition is WPM+IBA 0.2mg / L+sucrose 30g / L+agar 6.5~7g / L, the second-generation proliferation coefficient of '110R' is 3.6, and it germinates and takes root at the same time; the third-generation multiplication coefficient is 3.5, and the root system is well developed and The density of the main root is relatively high, and the leaves and roots are not browned. The steps of hydroponic seedling hardening and domestication and transplanting are the same as Example 1. The survival rate of '110R' is 85.1% in hydroponic and 90.0% in transplanting.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of plant tissue culture and seedling raising, in particular to a tissue culture and rapid propagation method for grape rootstocks. The method comprises thefollowing steps of taking dormant branches of grape rootstocks, shearing the dormant branches into single-bud stems, breaking physiological dormancy, carrying out water culture on the single-bud stems until the single-bud stems germinate, and sampling when new shoots are 10-20cm long so as to obtain explants; shearing wounds at the top end and the base part of the explant, and inoculating the explant into an initial culture medium for initial culture; when new shoots grow to 5-6 cm, inoculating the new shoots into a proliferation culture medium for proliferation culture, carrying out subculture once every 40-50 days, and carrying out subculture for 3-4 generations; and selecting tissue culture seedlings for seedling hardening and transplanting. According to the method, the initial culturemedium can obviously induce the stem segments of the grape rootstocks to grow new shoots or cluster buds; and the new shoots or cluster buds subjected to initial culture in the propagation culture medium can be subjected to propagation culture and simultaneously germinate and root to form seedlings through one-step induction, and only one propagation culture medium needs to be prepared, so that the propagation efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and seedling cultivation, in particular to a tissue culture and rapid propagation method for grape rootstocks. Background technique [0002] In recent years, my country's grape planting area has greatly increased, and the grape industry has developed rapidly. The traditional self-rooted seedlings are the main cultivation method in my country's grape cultivation, but grafted seedlings are gradually being valued due to their resistance to diseases and insect pests, especially the characteristics of resistance to phylloxera. In grapevine grafts, the shoot portion of the union of the two grafted together is called the scion, while the rootstock provides the root system. In addition to enhancing stress resistance, good rootstocks can also promote the growth of scions and affect the quality and flavor of grapes. Therefore, it is particularly important to obtain virus-free high-quality gra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/02A01G31/00
CPCA01G17/02A01G31/00A01H4/001A01H4/008
Inventor 何非朱元娣王军张明悦
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap