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A kind of amphiphilic polymer for promoting stem cell interface adhesion growth and its preparation method and application

A polymer, amphiphilic technology, applied in the field of polymers, can solve the problems of flexible modification of substrates, limitations of biofunctionalization, weak hydrophobicity, etc., to avoid cumbersome and low efficiency, and ensure the adhesion and growth of stem cells.

Active Publication Date: 2022-04-01
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biofunctionalization of hydrophobic substrates using amphiphilic block polymers has its limitations
For example, the hydrophobicity of polypropylene oxide is weak, which cannot meet the situation that requires strong hydrophobic interaction; the types of block parts are fixed, and it is difficult to flexibly change according to the properties of the substrate; any adjustment of the copolymerization ratio in block copolymers is difficult. Need to resynthesize the main chain

Method used

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  • A kind of amphiphilic polymer for promoting stem cell interface adhesion growth and its preparation method and application
  • A kind of amphiphilic polymer for promoting stem cell interface adhesion growth and its preparation method and application
  • A kind of amphiphilic polymer for promoting stem cell interface adhesion growth and its preparation method and application

Examples

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preparation example Construction

[0107] The preparation method of the present invention will be further described in detail in conjunction with specific examples below. It should be understood that the following examples are only for illustrating and explaining the present invention, and should not be construed as limiting the protection scope of the present invention. All technologies realized based on the above contents of the present invention are covered within the scope of protection intended by the present invention.

[0108] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the reagents and materials used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0109] The HEPES buffer used in the following examples is a buffer solution with a concentration of 100 mM and a pH of 7.5.

[0110] Optional means that the feature exists or does not exist, and also means that the feature must exist, but the sp...

Embodiment 1

[0111] Embodiment 1 Synthesis of amphiphilic graft polymer OA25-PLL-PEG3k-RGD50

[0112] Weigh 7.5mg poly-L-lysine hydrobromide (PLL, average molecular weight 2.2kDa, purchased from Shanghai Yuanye Company) in a 2ml centrifuge tube, dissolve it in 100μl HEPES buffer to obtain solution (1) .

[0113] Weigh 54mg maleimide-polyethylene glycol-succinimide ester (NHS-PEG3k-MAL, average molecular weight 3000Da, purchased from Sigma-aldrich company) (end group modification is respectively maleimide and succinimide Polyethylene glycol of imide ester) was dissolved in 100 μl N,N-dimethylformamide (analytical grade, purchased from Beijing Chemical Plant) to obtain solution (2).

[0114] Then weigh 12.6 mg of hexapeptide containing N-terminal acetyl-modified cysteine ​​and RGD sequence, its amino acid sequence is CGRGDS (Ac-CGRGDS, molecular weight 636, purchased from Nanjing KingScript Biotechnology Co., Ltd.), and Dissolve in 100 μl HEPES buffer to obtain solution (3).

[0115] 3.2 ...

Embodiment 2

[0120] Example 2 Synthesis of amphiphilic graft polymer OA25-PLL-PEG2k-RGD30

[0121] Similar to Example 1, the difference is that 20 mg of polyethylene glycol (NHS-PEG2k-MAL, average molecular weight 2000 Da, purchased from Sigma-aldrich Company) whose end groups are modified with maleimide and succinimide esters was weighed , which was dissolved in 100 μl N,N-dimethylformamide to obtain solution (2).

[0122] Weigh 7.6 mg of hexapeptide with N-terminal acetyl-modified cysteine ​​whose amino acid sequence is CGRGDS (Ac-CGRGDS, molecular weight 636, purchased from Nanjing GenScript Biotechnology Co., Ltd.), and dissolve it in 100 μl of HEPES buffer to obtain solution (3).

[0123] The lyophilized powder obtained was a functionalized amphiphilic polymer grafted with 25% oleic acid and 30% polyethylene glycol 2000-GRGDS, called OA25-PLL-PEG2k-RGD30.

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Abstract

The invention provides an amphiphilic polymer for promoting stem cell interfacial adhesion growth and its preparation method and application. The amphiphilic polymer introduces a hydrophilic component and a hydrophobic component, wherein the hydrophobic component is used to interact with materials The hydrophobic surface forms a stable surface coverage through hydrophobic interaction, and the hydrophilic component provides biocompatibility and contains peptides that promote the adhesion and growth of stem cells. The amphiphilic polymer is used to modify the surface of the hydrophobic substrate, so that it has good biocompatibility and can promote the long-term attachment and growth of stem cells and maintain their dryness.

Description

technical field [0001] The invention belongs to the field of polymers, and in particular relates to an amphiphilic polymer for promoting interfacial adhesion growth of stem cells, a preparation method and application thereof. Background technique [0002] Regenerative medicine requires the reliable cultivation of large quantities of stem cells and artificial tissues for the repair and replacement of tissues and organs. Biomedical materials that promote the adherent growth of cells and stem cells have been widely used in stem cell culture. The stem cell culture environment must meet its strict requirements on nutrients, growth factors, osmotic pressure, etc., which are specifically reflected in: (1) the culture substrate must be a hydrosol system; (2) the substrate must contain a variety of signals in a certain proportion. Pathway regulators, including transforming growth factor (TGF), Noggin and Wnt and other signaling proteins; (3) The culture substrate needs to have an el...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00C12N5/0775D06M15/37D06M101/32
CPCC08G81/00C12N5/0665D06M15/37C12N2533/30D06M2101/32
Inventor 喻长远李泽浩游长江许立达李政泰李丽丹
Owner BEIJING UNIV OF CHEM TECH
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