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Preparation method of bacterial cellulose composite collagen self-assembly material

A technology of bacterial cellulose and collagen, which is applied in the field of preparation of natural polymer materials, can solve the problems of complicated process, long preparation time, poor controllability, etc., and achieve improved swelling rate, excellent biocompatibility, and shortened preparation time Effect

Active Publication Date: 2021-03-05
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned existing preparation methods are mainly to add collagen in the biological culture process of bacterial cellulose to prepare composite bacterial cellulose membranes, all of which are based on the biological culture method and bacterial cellulose as the base to prepare membrane materials, and the preparation time is long and the process is difficult. Complex and less controllable

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  • Preparation method of bacterial cellulose composite collagen self-assembly material
  • Preparation method of bacterial cellulose composite collagen self-assembly material

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Effect test

Embodiment 1

[0026] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0027] (1) Dissolve the fish skin collagen powder in a phosphate buffer solution with a pH of 7.4 to form a 2000 μg / mL fish collagen solution;

[0028] (2) Add 200 mg of bacterial cellulose homogenate (10% solid content) into 10 mL of fish skin collagen solution, adjust the pH of the solution to 8.5, stir ultrasonically for 10 min, and then stand at 37°C for 12 h for self-assembly;

[0029] (3) Put the prepared bacterial cellulose composite fish collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8,000 to 14,000 Daltons, perform dialysis for 12 hours, and change the water every 4 hours;

[0030] (4) Add 1 mL of 5% glycerin and 0.1 mg of nano-silver particles to the bacterial cellulose composite fish collagen self-assembly solution after dialysis, and disperse with ultrasonic stirring for...

Embodiment 2

[0033] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0034] (1) Take an appropriate amount of bovine serum collagen powder and dissolve it in a phosphate buffer solution with a pH of 7.4 to form a bovine serum collagen solution of 1000 μg / mL;

[0035] (2) Mix 2.5 mg of bacterial cellulose dry powder with 50 mL of bovine serum collagen solution, adjust the pH of the solution to 8.0, ultrasonically disperse for 30 min, and then stand at 25°C for 24 h for self-assembly;

[0036] (3) Put bacterial cellulose composite bovine serum collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8,000-14,000 Daltons, and change the water every 4 hours for dialysis for 24 hours;

[0037] (4) Add 1.5 mL of 5% glycerin and 0.2 mg of nano-silver particles to the dialysis bacterial cellulose composite bovine serum collagen self-assembly solution, and disperse wit...

Embodiment 3

[0041] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0042] (1) dissolving rat tail collagen in a phosphate buffer solution with a pH of 7.4 to prepare a 100 μg / mL rat tail collagen solution;

[0043] (2) Mix 0.2 mg of bacterial cellulose dry powder with 10 mL of rat tail collagen solution, adjust the pH of the solution to 8.0, ultrasonically disperse for 30 min, and then stand at 35°C for 4 h for self-assembly;

[0044] (3) Put bacterial cellulose composite bovine serum collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8,000-14,000 Daltons, and change the water every 4 hours for dialysis for 24 hours;

[0045] (4) Add 0.2 mL of 5% glycerin and 0.05 mg of nano-silver particles to the dialysis bacterial cellulose composite rat tail collagen self-assembly solution, and disperse with ultrasonic stirring for 30 min;

[0046] (5) Add 0.5% modi...

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Abstract

The invention relates to a preparation method of a bacterial cellulose composite collagen self-assembly material. Bacterial cellulose adopted by the preparation method is a natural biopolymer, has a large length and is of a superfine net structure, and polyhydroxy active groups on the surface of the bacterial cellulose are combined with collagen through hydrogen bonds, so that the self-assembly process of collagen can be remarkably improved, the preparation time is effectively shortened, moreover, after collagen is adsorbed on the surface of bacterial cellulose and is self-assembled by takingbacterial cellulose as a core, a network-shaped structure can be integrally shown, and the swelling rate, the water retention rate, the plasticity and other properties of the collagen self-assembly material are improved. Besides, the bacterial cellulose and the collagen are both natural polymer materials and have excellent biocompatibility and biodegradability, the preparation method of the wholepreparation process is simple and controllable, surface modification and chemical modification are not needed, and the preparation method is free of residues, free of toxicity and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of preparation of natural polymer materials, in particular to a preparation method of a bacterial cellulose composite collagen self-assembly material. Background technique [0002] Collagen is the main component of biological connective tissue. It has good biocompatibility, biodegradability and weak antigenicity. Under certain conditions, collagen can spontaneously assemble in vitro to form a highly ordered network structure. The self-assembly of collagen can improve the biological and mechanical properties of collagen, and it is significantly better than the performance of collagen monomer molecules in terms of enzyme degradation resistance and biomechanical properties, which is beneficial to cell adhesion, proliferation, Diffusion and Migration. [0003] The Chinese invention patent application "A Method for Collagen Self-Assembly" with the application number CN201611153878.4 self-assembles collagen with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C08L1/04C08K13/02C08K5/053C08K3/08C08K5/07
CPCC08J3/246C08J2389/00C08J2301/04C08J2401/04C08K13/02C08K5/053C08K5/07C08K2201/011C08K2003/0806
Inventor 李亚姚利辉王亚娟仇丹王灵辉
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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