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A kind of preparation method of bacterial cellulose composite collagen self-assembly material

A technology of bacterial cellulose and collagen, applied in the field of preparation of natural polymer materials, can solve the problems of poor controllability, complex process, long preparation time, etc., and achieves improved swelling rate, excellent biocompatibility, and shortened preparation time. Effect

Active Publication Date: 2022-06-24
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned existing preparation methods are mainly to add collagen in the biological culture process of bacterial cellulose to prepare composite bacterial cellulose membranes, all of which are based on the biological culture method and bacterial cellulose as the base to prepare membrane materials, and the preparation time is long and the process is difficult. Complex and less controllable

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  • A kind of preparation method of bacterial cellulose composite collagen self-assembly material
  • A kind of preparation method of bacterial cellulose composite collagen self-assembly material

Examples

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Effect test

Embodiment 1

[0026] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0027] (1) Dissolve fish skin collagen powder in a phosphate buffer solution with a pH of 7.4 to prepare a fish collagen solution of 2000 μg / mL;

[0028] (2) 200 mg of bacterial cellulose homogenate (solid content of 10%) was added to 10 mL of fish skin collagen solution, the pH of the solution was adjusted to 8.5, and the solution was ultrasonically stirred for 10 min and left to stand at 37°C for 12 h for self-assembly;

[0029] (3) put the prepared bacterial cellulose composite fish collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8000-14000 Daltons, dialyze for 12 hours, and change the water every 4 hours;

[0030] (4) Add 1 mL of 5% glycerol and 0.1 mg of silver nanoparticles to the self-assembly solution of bacterial cellulose composite fish collagen after dialysis, and ultrason...

Embodiment 2

[0033] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0034] (1) Dissolve an appropriate amount of bovine serum collagen powder in a phosphate buffer solution with a pH of 7.4 to prepare a bovine serum collagen solution of 1000 μg / mL;

[0035] (2) Mix 2.5 mg of bacterial cellulose dry powder with 50 mL of bovine serum collagen solution, adjust the pH of the solution to 8.0, and then disperse by ultrasonic for 30 minutes and then stand at 25°C for 24 hours for self-assembly;

[0036] (3) Put the bacterial cellulose composite bovine serum collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8000-14000 Daltons, and change the water for dialysis every 4h for 24h;

[0037] (4) Add 1.5 mL of 5% glycerol and 0.2 mg of silver nanoparticles to the self-assembly solution of bacterial cellulose combined with bovine serum collagen after dialysis, and ul...

Embodiment 3

[0041] The preparation method of the bacterial cellulose composite collagen self-assembly material of the present embodiment comprises the following steps:

[0042] (1) Dissolve rat tail collagen in a phosphate buffer solution with a pH of 7.4 to prepare a rat tail collagen solution of 100 μg / mL;

[0043] (2) Mix 0.2 mg of dry bacterial cellulose powder with 10 mL of rat tail collagen solution, adjust the pH of the solution to 8.0, and then disperse by ultrasonic for 30 minutes and then stand at 35°C for 4 hours for self-assembly;

[0044] (3) Put the bacterial cellulose composite bovine serum collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8000-14000 Daltons, and change the water for dialysis every 4h for 24h;

[0045] (4) Add 0.2 mL of 5% glycerol and 0.05 mg of silver nanoparticles to the self-assembly solution of bacterial cellulose composite rat tail collagen after dialysis, and ultrasonically stir and disperse for 30 min;

[0046] (5...

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Abstract

The invention relates to a preparation method of a bacterial cellulose composite collagen self-assembly material. The bacterial cellulose used in the invention is a natural biopolymer, which itself has a relatively large length and an ultrafine network structure. The polyhydroxyl active groups on the surface of bacterial cellulose are combined with collagen by hydrogen bonding, which can not only significantly improve the self-assembly process of collagen and effectively shorten the preparation time, but also, collagen is adsorbed on the surface of bacterial cellulose with bacterial cellulose as the core After self-assembly, it will present a network structure as a whole, which improves the swelling rate, water retention rate, plasticity and other properties of the collagen self-assembly material; in addition, the bacterial cellulose and collagen of the present invention are both natural polymer materials, All have excellent biocompatibility and biodegradability, the preparation method of the whole preparation process is simple and controllable, no need for surface modification and chemical modification, no residue, no toxicity, and environmental protection.

Description

technical field [0001] The invention relates to the technical field of preparation of natural polymer materials, in particular to a preparation method of a bacterial cellulose composite collagen self-assembly material. Background technique [0002] Collagen is the main component of biological connective tissue, with good biocompatibility, biodegradability and weak antigenicity. Under certain conditions, collagen can spontaneously assemble in vitro to form a highly ordered network structure. The self-assembly of collagen can improve the biological and mechanical properties of collagen, and it is significantly better than the performance of collagen monomer molecules in terms of enzymatic degradation resistance and biomechanical properties, which is conducive to cell adhesion, proliferation, Diffusion and Migration. [0003] The Chinese invention patent application with the application number of CN201611153878.4, "A method for collagen self-assembly", self-assembles collagen ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08L89/00C08L1/04C08K13/02C08K5/053C08K3/08C08K5/07
CPCC08J3/246C08J2389/00C08J2301/04C08J2401/04C08K13/02C08K5/053C08K5/07C08K2201/011C08K2003/0806
Inventor 李亚姚利辉王亚娟仇丹王灵辉
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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