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Chitin nanocrystalline composite collagen self-assembly method

A chitin nanocrystal, collagen technology, applied in the direction of cellulose/protein conjugated rayon, textile and papermaking, conjugated rayon, etc., can solve the problem of single structure and function of self-assembled products, self-assembly of collagen Harsh conditions and other problems, to achieve the effect of improving the self-assembly process, the reaction process is simple and controllable, and the reaction conditions are simple

Active Publication Date: 2020-12-15
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the conditions for self-assembly of a single collagen are harsh, and the self-assembly product has a single structure and function

Method used

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  • Chitin nanocrystalline composite collagen self-assembly method
  • Chitin nanocrystalline composite collagen self-assembly method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) configure the chitin nanocrystal aqueous solution of 20 μg / mL;

[0027] (2) Take fish skin collagen and dissolve it in water, and use a phosphate buffer solution with a pH of 7.4 to prepare a 200 μg / mL fish skin collagen solution;

[0028] (3) 1mL chitin nanocrystal solution was ultrasonically dispersed and mixed with 5mL fish skin collagen solution, stirred ultrasonically for 10min, and then left at 40°C for 5.5h for self-assembly;

[0029] (4) Put the prepared chitin nanocrystal composite fish skin collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8,000 to 14,000 Daltons, and change the water every 4 hours for dialysis for 24 hours;

[0030] (5) After centrifuging the chitin nanocrystal composite fish skin collagen self-assembly solution after dialysis, take out the lower precipitate, add 1 mL of 2% glutaraldehyde solution, fix it for 120 min, wash it with water for 3 times, and freeze-dry it in a freeze dryer . The UV spectr...

Embodiment 2

[0032] (1) configure the chitin nanocrystal aqueous solution of 100 μg / mL;

[0033] (2) After dissolving the bovine tendon collagen in water, use a phosphate buffer solution with a pH of 7.4 to configure a 1000 μg / mL bovine collagen solution;

[0034] (3) 1.5 mL of chitin nanocrystal solution was ultrasonically dispersed and mixed with 5 mL of bovine tendon collagen solution, stirred ultrasonically for 10 min, and then left at 35°C for 6 h for self-assembly;

[0035] (4) The prepared chitin nanocrystal composite bovine tendon collagen self-assembly solution is packed into a dialysis bag with a molecular weight cut-off of 8000-14000 Daltons, and the water is changed every 4 hours for dialysis for 24 hours;

[0036] (5) After centrifuging the dialyzed chitin nanocrystal composite bovine bone collagen self-assembly solution, take out the lower precipitate, add 1.5% formaldehyde solution 1.5mL to fix it for 100min, wash it with water three times, and freeze-dry it in a freeze drye...

Embodiment 3

[0038] (1) configure the chitin nanocrystal aqueous solution of 1 μg / mL;

[0039] (2) Take rat tail collagen and dissolve it in glacial acetic acid, and use a phosphate buffer solution with a pH of 7.4 to prepare a 20 μg / mL rat tail collagen solution;

[0040] (3) 1mL chitin nanocrystal solution was ultrasonically dispersed and mixed with 5mL rat tail collagen solution, stirred ultrasonically for 10min, and then left at 37°C for 2h for self-assembly;

[0041] (4) Put the prepared chitin nanocrystal composite rat tail collagen self-assembly solution into a dialysis bag with a molecular weight cut-off of 8,000 to 14,000 Daltons, and change the water every 2 hours for dialysis for 12 hours;

[0042] (5) After centrifuging the chitin nanocrystal composite rat tail collagen self-assembly solution after dialysis, take out the lower sediment, add 0.5% modified glutaraldehyde solution 0.5mL and fix it for 100min, wash it with water 3 times, and then put it in a freeze dryer Freeze-dr...

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Abstract

The invention discloses a chitin nanocrystalline composite collagen self-assembly method. The chitin nanocrystalline composite collagen self-assembly method is characterized by comprising the following steps: (1), preparing 1-100 micrograms / mL chitin nanocrystalline aqueous solution; (2), dissolving collagen in water or glacial acetic acid, and preparing a collagen solution with the concentrationof 50-1500micrograms / mL by using a buffer solution; (3), mixing the chitin nanocrystalline solution with the collagen solution, performing ultrasonic stirring, and performing self-assembly for 2-24 hours at the constant temperature of 25-50 DEG C to obtain a chitin nanocrystalline composite collagen self-assembly solution; (4), performing dialysis; and (5), performing centrifuging, taking out lower-layer precipitate, adding a cross-linking agent, performing fixing for 20-240 minutes, and carrying out freeze drying to obtain the required chitin nanocrystalline composite collagen self-assembly product. Compared with the prior art, the method has the advantages that the collagen self-assembly process can be improved, and the performance of a collagen self-assembly product can be improved.

Description

technical field [0001] The invention relates to the technical field of natural polymer self-assembly, in particular to a method for self-assembly of chitin nanocrystal composite collagen. Background technique [0002] Collagen is the main component of biological connective tissue, and its structural feature is a triple helix structure formed by three peptide chains winding. Collagen has good biocompatibility, biodegradability and weak antigenicity, and is widely used in biomaterials, food science, medical cosmetology and other fields. Self-assembly is one of the most important molecular behaviors of collagen. Under certain conditions, collagen can spontaneously assemble in vitro to form a highly ordered network structure. The self-assembly of collagen has a significant impact on the biological and mechanical properties of collagen. The improvement effect is significantly better than that of collagen monomer molecules in terms of enzyme degradation resistance and biomechanic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C08L5/08D01F8/02D01F8/18C08J5/18
CPCC08J3/246C08J5/18D01F8/02D01F8/18C08J2389/00C08J2405/08
Inventor 李亚王文娴姚利辉王亚娟仇丹
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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