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Structurally modified antibacterial peptide BMAP-14 and application thereof

A technology of BMAP-18 and antimicrobial peptide, applied in the field of protein engineering, can solve the problem of low hemolysis rate, achieve low hemolysis rate, retain broad-spectrum antibacterial properties, and good heat resistance

Active Publication Date: 2021-03-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, an antimicrobial peptide with a low hemolytic rate was disclosed in an article titled "Research on Bacteriostatic Activity and Hemolysis of New Hemoglobin Antimicrobial Peptides" (Advances in Animal Medicine, 2014, 35(9): 54-57), but in When the antimicrobial peptide concentration was 2 mg / mL (2000 μg / uL), the hemolysis rate also reached 6.7%, still exceeding the safety threshold

Method used

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  • Structurally modified antibacterial peptide BMAP-14 and application thereof
  • Structurally modified antibacterial peptide BMAP-14 and application thereof
  • Structurally modified antibacterial peptide BMAP-14 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Construction of BMAP-14

[0019] 1. Use I-TASSER to predict and establish the spatial structure of the unmodified antimicrobial peptide BMAP-18; design BMAP-14 with 4 amino acids reduced at the C-terminus of BMAP-18, and its amino acid sequence is shown in SEQ ID NO.1; At the same time, the C-terminus of BMAP-18 was designed to reduce 5, 3, and 2 polypeptides, respectively named as BMAP-13, BMAP-15, and BMAP-16. According to I-TASSER prediction, the spatial structure of each designed polypeptide is as follows: figure 1 shown. figure 1 Among them, from A to B, respectively correspond to: BMAP-13, BMAP-14, BMAP-15, BMAP-16, BMAP-18.

[0020] From figure 1 It can be seen that the BMAP-14 provided by the present invention is closest to the N-terminus of BMAP-18, and basically keeps the N-terminus of BMAP-18 unchanged, suggesting that it may maintain the antibacterial activity of the parent antimicrobial peptide BMAP-18; The spatial structures of the polypepti...

Embodiment 2

[0022] Embodiment 2: BMAP-14 performance detection

[0023] 1. Detection of antibacterial activity.

[0024] (1) Using the broth microdilution antibacterial test (M7-A8) of the American Clinical Laboratory Standardization Institute (CLSI), the minimum inhibitory concentration (MIC value) of BMAP-14 and BMAP-18 to bacteria was determined, and the culture The base is calcium-adjusted MH broth medium.

[0025] (2) The minimum inhibitory concentration of BMAP-14 and BMAP-18 against Candida albicans was determined by using the broth microdilution method anti-yeast-like fungal susceptibility test (M27-A2) of the American Clinical Laboratory Standardization Institute (CLSI) (MIC value), the detection medium is Sabouraud glucose liquid medium.

[0026] (3) The detection results are shown in Table 1. In Table 1, "ND" means that no activity was detected, and " / " means that no corresponding test was performed.

[0027] Table 1 The MIC value (μg / mL) of each antimicrobial peptide and c...

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Abstract

The invention belongs to the field of protein engineering, and particularly relates to a structurally modified antibacterial peptide BMAP-14 and application thereof. The specific technical scheme is as follows: the amino acid sequence of the antibacterial peptide BMAP-14 is shown as SEQ ID NO. 1. The invention provides a novel modified antibacterial peptide, which retains the N-terminal structureof BMAP-18, retains the broad-spectrum antibacterial property, has good heat resistance, certain plasma stability and extremely low hemolysis, and can be widely applied to the antibacterial field.

Description

technical field [0001] The invention belongs to the field of protein engineering, and in particular relates to a structurally modified antimicrobial peptide BMAP-14 and its application. Background technique [0002] The development of pathogen resistance to antibiotics is a serious threat to global public health; one of the solutions is to find novel antimicrobial substances. Antimicrobial peptides have the characteristics of small molecular weight, good water solubility, low antigenicity, and high stability, especially the characteristics that are not easy to produce drug resistance. Although its antibacterial effect is slightly lower than that of traditional antibiotics, it is still considered an important option to replace existing antibiotics when drug-resistant pathogens emerge in large numbers. Currently, a variety of antimicrobial peptides are under feasibility study. Among them, cathelicidin antimicrobial peptides are widely distributed in the human body, as well a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A61P31/10
CPCC07K7/08A61P31/04A61P31/10A61K38/00Y02A50/30
Inventor 刘炯宇江建平
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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