Graphene product and therapeutic uses thereof
A graphene and product technology, applied in the field of graphene products and their therapeutic uses, can solve problems such as toxic biocompatibility
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Embodiment 1
[0095] Preparation and physical and chemical characterization of GMC-1
[0096] raw material
[0097] The structural characteristics, degree of graphitization, and main physicochemical properties and thermal properties of the raw materials that can be used to prepare the graphene nanomaterials of the present invention will be reflected in Table 1 below.
[0098] Table 1: Physicochemical properties of GNFs raw materials
[0099]
[0100] a Number of graphene faces in the crystal (npg=Lc / d);
[0101] d is the alternating range of thin layers; Lc is the average size of crystals in the sample in the direction perpendicular to the graphene basal plane
[0102] b I D / I G : The quotient between the D-band and G-band intensities in the Raman spectrum.
[0103] In this example, graphene nanofibers (GNFs) were used to prepare the materials described in the present invention.
[0104] The graphene-based carbon nanomaterials (GNFs) undergo a purification process using HF to...
Embodiment 2
[0151] Embodiment 2: HEP-G2 cell line toxicity test
[0152] We performed pharmacological and toxicological studies with GMC-1 on cultured cells (hepatic cell line Hep-G2).
[0153] In a 6-well dish, 300,000 cells per well were cultured in medium (DMEM by default) supplemented with 10% fetal bovine serum. cells at 37°C, 5% CO 2 and cultured in a controlled incubator under humidity conditions. Serum was removed 16 hours after reaching cell confluency. The product was added as a suspension at different concentrations in the same final volume for 24 hours maintaining the same controlled conditions. Subsequently, the cells are processed and additionally tested for viability / toxicity by the two tests indicated in the ISO above:
[0154] a) trypan blue staining exclusion method to distinguish live / dead cells, and
[0155] b) MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) cell staining method to measure metabolic activity.
[0156] The result is as Figure 5 shown....
Embodiment 3
[0157] Example 3: Wound Healing in Animal Models
[0158] Skin wound healing is a common regenerative process that closes wounds and rebuilds the epithelial barrier and underlying connective tissue.
[0159] Numerous factors and systemic conditions are causative agents that impair skin function. After skin injury, new epithelial cells must form to allow the wound to heal.
[0160] Wound healing involves cell migration, proliferation, differentiation and formation of extracellular matrix. Chronic wounds, such as venous, diabetic foot or pressure ulcers, characterized by incomplete healing processes, including increased bacterial colonization and / or infection, are caused by other conditions such as diabetes, trauma, inflammatory diseases, metabolic abnormalities, Bleeding disorders, smoking, immunosuppression and malnutrition, obesity.
[0161] We performed skin regeneration experiments in a rodent wound healing model. The hydrogel with gelatin as the main excipient compon...
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