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Coated and covalently immobilized heparin hemoperfusion adsorbent and preparation method thereof

A technology of covalent immobilization and hemoperfusion, which is applied in the direction of blood circulation treatment, chemical instruments and methods, suction devices, etc., can solve the problems of poor blood compatibility, achieve a firm capsule, improve blood compatibility, and improve blood compatibility. capacitive effect

Pending Publication Date: 2021-03-12
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, in order to overcome at least one of the above-mentioned deficiencies in the prior art, the present invention uses polyphenolic compounds and polyamine compounds to co-deposit coatings on the surface of hemoperfusion adsorbents, and further covalently immobilizes heparin to solve the problem of adsorbents. Poor blood compatibility and particle shedding problems, while improving the anticoagulant performance of the adsorbent

Method used

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  • Coated and covalently immobilized heparin hemoperfusion adsorbent and preparation method thereof
  • Coated and covalently immobilized heparin hemoperfusion adsorbent and preparation method thereof

Examples

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Effect test

Embodiment 1

[0028] Add 0.075g of dopamine hydrochloride and 0.075g of ethylenediamine into 150mL of purified water to prepare 150mL of an aqueous solution containing 0.5g / L dopamine and 0.5g / L of ethylenediamine, and put 100ml of hemoperfusion adsorbent (spherical resin for small and medium molecule toxin adsorption) into the solution, adding an appropriate amount of ammonia water to make the pH of the solution 10, stirring slowly for 24 hours, then fully rinsing with normal saline, and draining to prepare an adsorbent coated with amino groups;

[0029] Dissolve 15 mg of heparin sodium in 0.05 M PBS buffer solution (pH is 6) to prepare 150 milliliters of heparin solution with a concentration of 0.1 g / L, then add 1.438 g of EDC and 0.863 g of NHS and stir to dissolve it, so that the EDC and NHS The concentration is 0.05mol / L, add 100ml of the adsorbent with amino groups, and stir slowly for 24 hours to obtain the adsorbent that is coated and covalently immobilized with heparin. Finally, th...

Embodiment 2

[0031] Add 0.3g of dopamine hydrochloride and 0.3g of diethylenetriamine into 150mL of purified water to prepare 150mL of an aqueous solution containing 2g / L dopamine and 2g / L of diethylenetriamine, and put 100ml of hemoperfusion adsorbent (spherical Resin, used for the adsorption of small and medium-sized molecule toxins) into the solution, adding an appropriate amount of Tris (Tris) to make the pH of the solution 8.5, stirring slowly for 24 hours, then fully rinsing with normal saline, and draining to prepare Adsorbents coated with amino groups;

[0032]Dissolve 75mg of heparin sodium in 0.05M MES buffer solution (pH is 5.5) to prepare 150 milliliters of heparin solution with a concentration of 0.5g / L, then add 2.157gEDC and 1.295gNHS and stir to dissolve it, so that the EDC and NHS The concentration is 0.075mol / L, add 100ml of the adsorbent with amino groups, and stir slowly for 24 hours to obtain the adsorbent that is coated and covalently immobilized heparin. Finally, th...

Embodiment 3

[0034] Add 0.75g dopamine hydrochloride and 0.75g triethylenetetramine to 150mL purified water to prepare 150mL aqueous solution containing 5g / L dopamine and 5g / L triethylenetetramine, and put 100ml hemoperfusion adsorbent (spherical resin, For the adsorption of small and medium molecular toxins) into the solution, add an appropriate amount of sodium carbonate to make the pH of the solution 8.0, stir slowly for 24 hours, then fully rinse with normal saline, and drain to prepare the coated adsorbent with amino groups ;

[0035] Dissolve 150 mg of heparin sodium in 0.05 M citric acid buffer solution (pH is 4) to prepare 150 milliliters of heparin solution with a concentration of 1 g / L, then add 2.876 g of EDC and 1.726 g of NHS and stir to dissolve it, so that the EDC and NHS The concentration is 0.1mol / L, add 100ml of the adsorbent with amino groups, and stir slowly for 24 hours to obtain the adsorbent that is coated and covalently immobilized heparin. Finally, the adsorbent i...

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Abstract

The invention relates to the technical field of blood purification, and discloses a coated and covalently immobilized heparin hemoperfusion adsorbent and a preparation method thereof. Through the codeposition effect of polyphenol and polyamine compounds, the surface and the interior of pores of the hemoperfusion adsorbent are coated while a hydrophilic interface of polyamino is generated, and finally under the weak acid condition and in the presence of EDC and NHS, the hydrophilic interface reacts with carboxyl of heparin molecules to covalently immobilize heparin on the hemoperfusion adsorbent, so that the anticoagulation performance of the hemoperfusion adsorbent is improved. According to the invention, the hydrophobic surface of the hemoperfusion adsorbent is improved, amino is introduced to improve the hydrophilicity of the hemoperfusion adsorbent, and the blood compatibility of the hemoperfusion adsorbent is improved; particularly, heparin is covalently fixed on the surface of thehemoperfusion adsorbent, so that a blood coagulation phenomenon occurring when blood is in contact with the adsorbent can be reduced; and the hemoperfusion adsorbent is firm in coating, the fixed heparin is not prone to falling off, the hemoperfusion adsorbent is suitable for various clinically-used heparin perfusion products at present, and the safety of clinical application is improved.

Description

technical field [0001] The invention relates to the technical field of blood purification, more specifically, to a hemoperfusion adsorbent coated and covalently immobilized with heparin and a preparation method thereof. Background technique [0002] Hemoperfusion adsorbent is a porous and high specific surface area medical consumable, which is mainly used for the adsorption of small and medium molecular toxins, the adsorption of bilirubin for liver disease and the adsorption of pathogenic factors in the field of immunity. At present, the main application modes are single hemoperfusion mode, hemodialysis combined with hemoperfusion mode and plasma separation and adsorption mode. [0003] Hemoperfusion adsorbents are generally spherical resin or spherical activated carbon. During clinical use, blood cells (such as platelets) in the blood will adhere and gather on the surface of the adsorbent, causing coagulation. In addition, if the adsorbent is broken, it will shed particles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/32B01J20/20A61M1/36
CPCB01J20/3217B01J20/3272B01J20/20A61M1/3679A61M1/3672A61M2202/0413A61M2202/0021
Inventor 张旭锋孙丽芬
Owner YUNNAN NORMAL UNIV
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