Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL as well as preparation method and application of bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL

A bioactive peptide, active technology, applied in the field of protein to achieve the effect of improving quality, improving ability, and reducing the incidence of the body

Active Publication Date: 2021-03-12
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Protein tyrosine phosphatasereceptor type C-associated protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL as well as preparation method and application of bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL
  • Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL as well as preparation method and application of bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL
  • Bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL as well as preparation method and application of bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The artificial synthesis of embodiment 1 bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL

[0033] 1. Synthesis of Bioactive Peptides

[0034] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0035] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0036] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0037] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successf...

Embodiment 2

[0074] The immune activity experiment of embodiment 2 bioactive peptides

[0075] 1. Experiment on the ability of bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL to promote macrophage phagocytosis of neutral red

[0076] 1. Experimental reagents and instruments:

[0077] Reagent: experimental animal balb / c mouse (male 6-8 weeks old) Animal Experiment Center, College of Agriculture and Biology, Shanghai Jiaotong University; mouse spleen lymphocyte-derived bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL obtained in Example 1; LPS, purchased from Sigma ; Neutral red staining solution, produced by Biyuntian Institute of Biotechnology.

[0078] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO2 Incubator Heraeus; Dragon WellscanMK3 microplate reader Labsystems.

[0079] 2. Experimental method:

[0080] The number of cells added is 2×10 6 100 μl / well of ...

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL as well as a preparation method and application thereof, and the amino acid sequence of thebioactive peptide SGSAAWDDSAGGAGGQGLRVTAL is Ser-Gly-Ser-Ala-Ala-Trp-Asp-Asp-Ser-Ala-Gly-Gly-Ala-Gly-Gly-Gln-Gly-Leu-Arg-Val-Thr-Ala-Leu. An in-vitro immunoregulation function experiment shows that the bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL has a relatively good immunoregulation function. The invention further discloses a preparation method of the bioactive peptide SGSAAWDDSAGGAGGQGLRVTAL. Thebioactive peptide SGSAAWDDSAGGAGGQGLRVTAL can promote macrophage activation, release cytokines and improve the external pathogen infection resistance of an organism, has a remarkable promoting effecton the neutral red phagocytosis capacity of in-vitro macrophages under the condition that inflammation occurs, can reduce the incidence rate of the organism, improves the life quality, and has very important significance in developing food, health care products and medicines with immunoregulation functions.

Description

technical field [0001] The invention relates to the field of protein, in particular to a biologically active peptide SGSAAWDDSAGGAGGQGLRVTAL and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉李政郭婷婷栾媛媛占文静张伯宇谢雨佳王娟
Owner SHANGHAI JIAO TONG UNIV
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