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Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and application of lactobacillus rhamnosus

A technology of Lactobacillus rhamnosus and ulcerative colitis, applied in the field of functional microorganisms, can solve problems such as individual differences, achieve the effects of increasing abundance, wide application prospects, and improving the diversity of intestinal flora

Active Publication Date: 2021-03-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus is the most common probiotic, safe and edible, without side effects. A large number of studies have shown that some special Lactobacillus strains have good anti-inflammatory and intestinal flora effects, but this effect is obvious in Lactobacillus individual differences

Method used

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  • Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and application of lactobacillus rhamnosus
  • Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and application of lactobacillus rhamnosus
  • Lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and application of lactobacillus rhamnosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Lactobacillus rhamnosus CCFM1128 has good tolerance to simulated gastrointestinal fluid

[0050] The cryopreserved Lactobacillus rhamnosus CCFM1128 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and then subcultured in mMRS medium for 2~ After 3 times, the culture solution of Lactobacillus rhamnosus CCFM1128 was obtained.

[0051] Take 1 mL of the culture solution of Lactobacillus rhamnosus CCFM1128, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH = 2.5), and culture it anaerobically at 37°C. Samples were taken at 0.5h, 1h, 2h, and 3h, and the mMRS agar medium was poured to culture the plate colonies, and the number of viable bacteria was determined and the survival rate was calculated. The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the nu...

Embodiment 2

[0058] Example 2: Lactobacillus rhamnosus CCFM1128 has no toxic side effects on C57BL / 6J mice

[0059] Resuspend Lactobacillus rhamnosus CCFM1128 in 3g / 100mL sucrose solution to make a concentration of 5.0×10 9 CFU / mL bacterial suspension. Take 6 healthy male C57BL / 6J mice with a body weight of about 14-16g. After adapting to the environment for a week, they are given the bacterial suspension of this concentration by gavage once a day (0.2mL per gavage), observed for a week, and recorded the death and body weight. .

[0060] The results of these tests are listed in Table 3. These results indicated that feeding concentrations of 5.0×10 9 CFU / mL of Lactobacillus rhamnosus CCFM1128 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0061] Table 3 Changes in body weight and death of mice

[0062] time (days) 1 2 3 4 5 6 7 weight(g) 19.22±0.10 19.40±0...

Embodiment 3

[0064] Example 3: Effect of Lactobacillus rhamnosus CCFM1128 on disease symptoms in UC mice

[0065] Take 30 healthy male C57BL / 6J mice weighing 14-16g, adapt to the environment for 1 week, 6 mice in each group, and randomly divide them into 5 groups: blank group, model group, drug group, Lactobacillus rhamnosus CCFM1128 intervention group (CCFM1128), Lactobacillus rhamnosus FNMGEL5-1 control group (FNMGEL5-1). The dose of intragastric bacterial suspension was 5.0×10 9 CFU / mL, resuspended in 3% (w / v) sucrose solution. At week 5, dextran sodium sulfate (dextran sulphate sodium, DSS) at a final concentration of 2.5 g / 100 mL (ie 2.5% (w / v)) was added to drinking water to induce colitis in mice. The grouping and treatment methods of experimental animals are shown in Table 4:

[0066] Table 4 Animal experiment design

[0067]

[0068] In the fifth week, during the modeling period (DSS treatment period), the body weight of the mice was regularly weighed every day and the perc...

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Abstract

The invention discloses lactobacillus rhamnosus capable of preventing and relieving ulcerative colitis and application of the lactobacillus rhamnosus, and belongs to the technical field of functionalmicroorganisms. Lactobacillus rhamnosus CCFM1128 can tolerate the gastrointestinal environment of a human body, remarkably improve weight loss during ulcerative colitis, improve fecal traits and hematochezia conditions, reduce colon mucosal injury, reduce MPO activity, reduce generation of proinflammatory factors TNF-alpha, IL-1beta, IL-6 and IFN-gamma in colon, reduce the content of proinflammatory factors IL-6 in serum, up-regulate the gene transcription level of colon close connection related proteins Claudin-3, ZO-1, ZO-2 and Occludin, up-regulate the transcription level of antibacterial peptide CRAMP in the colon, increase the content of short-chain fatty acid in excrement, improve the diversity of intestinal flora, and improve the abundance of short-chain fatty acid producing bacteria Coprococcus in the intestinal tract.

Description

technical field [0001] The present invention is a strain of Lactobacillus rhamnosus capable of preventing and alleviating ulcerative colitis and its application, specifically relates to the application of Lactobacillus rhamnosus CCFM1128 in the preparation of functional bacterial agents, food and / or medicine, which belongs to functional microorganisms technology field. Background technique [0002] In Western countries, inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a common autoimmune disease of the digestive system, and its incidence rate is as high as 5-12 patients per 100,000 population. With the increasing pressure of Chinese people's life and changes in dietary patterns, the incidence of inflammatory bowel disease in China is increasing, which is more than 15 times higher than 20 years ago. Therefore, more and more research institutions and scholars have studied the pathogenesis and Treatment methods are explored in depth. Ulcerative colitis (UC) is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/04C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P1/04A23V2002/00A23V2400/175A23V2200/32A23V2200/3202
Inventor 王刚郑雨星王琳琳翟齐啸陆文伟崔树茂杨波毛丙永唐鑫赵建新张灏陈卫
Owner JIANGNAN UNIV
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