A kind of piezoelectric support and its preparation method and application

A technology of piezoelectric and drug-loaded microspheres, which is applied in the fields of biomedical engineering and biomedical materials, and can solve problems such as residual harmful substances, endangering human health, and affecting the removal of antigenic substances

Active Publication Date: 2022-06-24
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acellular matrix can only provide short-term release of the drug, and cannot release the drug for a long time to continuously stimulate the "focus" site, making it difficult for the acellular matrix to treat the defective tissue alone
Moreover, different decellularization methods directly affect the composition and ultrastructure of the obtained acellular matrix, thereby affecting its performance.
[0006] At present, the decellularization of bone to prepare the acellular matrix usually uses organic solvents such as chloroform and methanol mixture, and because the micropores of bone tissue are hydrophilic, this will hinder the contact between the micropores and the organic solvent, thereby affecting the potential. The removal of antigenic substances during the treatment process will affect the performance of the acellular matrix; in addition, the organic solvents adsorbed on the deep surface of the micropores during the treatment process may not be completely removed during the cleaning process, which will cause harmful substances to remain. May be harmful to human health

Method used

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  • A kind of piezoelectric support and its preparation method and application
  • A kind of piezoelectric support and its preparation method and application
  • A kind of piezoelectric support and its preparation method and application

Examples

Experimental program
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Effect test

preparation example Construction

[0036] (1) Preparation of porous barium titanate

[0037] The porous barium titanate used in the following piezoelectric stent preparation process is obtained by the following method:

[0038] Dissolve 1.2g of cetyltrimethylammonium bromide in 120ml of 75mol / l hydrogen chloride aqueous solution to obtain the first solution; dissolve 9.2ml of titanium tetrachloride in a mixed solution of 100ml of ethanol and 50ml of deionized water, The second solution was obtained; the second solution, 50ml of 1.5mol / l barium chloride aqueous solution and 25g of sodium hydroxide were added to the first solution at intervals of 6h under continuous stirring, and the stirring was stopped after 24h, and refluxed at 90°C for 5h , the product was collected by filtration, washed three times with deionized water and ethanol respectively, and suction filtered to obtain porous barium titanate powder.

[0039] (2) Preparation of piezoelectric stents

Embodiment 1

[0041] A piezoelectric support, its preparation method comprises the following steps:

[0042] S1. Preparation of decellularized matrix, including: taking 8-week-old cow's upper limb subchondral bone trabeculae, washing with high pressure water; then decellularizing PBS buffer solution containing 0.2% EDTA for 1 h at room temperature, containing Soak in 10mM Tris buffer of 0.3% sodium dodecyl sulfate for 6h, wash with PBS for several times until there are no air bubbles; then use supercritical carbon dioxide technology to process it, set the minimum pressure to 6MPa, the maximum pressure to 16MPa, and the system temperature to be 37°C, specifically exposed to the maximum pressure for 10min, the flow rate is 10kg / h, and then reduced to normal pressure within 6min to obtain an acellular matrix with a three-dimensional porous structure;

[0043] S2. The preparation of drug-loaded microspheres includes: mixing 10 mg of BMP-2 with 100 mg of porous barium titanate to obtain a mixed ...

Embodiment 2

[0046] A piezoelectric support, its preparation method comprises the following steps:

[0047] S1. Preparation of decellularized matrix, including: taking 4-week-old cow's upper limb subchondral bone trabeculae, washing with high pressure water; then decellularizing PBS buffer solution containing 0.3% EDTA for 2 hours at room temperature, containing Soak in 10mM Tris buffer of 0.1% sodium dodecyl sulfate for 6h, wash with PBS for several times until there are no air bubbles; then use supercritical carbon dioxide technology to process it, set the minimum pressure to 8MPa, the maximum pressure to 20MPa, and the system temperature to be 37°C, specifically exposed to the maximum pressure for 12min, the flow rate is 10kg / h, and then reduced to normal pressure within 5min to obtain an acellular matrix with a three-dimensional porous structure;

[0048] S2. The preparation of drug-loaded microspheres includes: mixing 10 mg of sodium alendronate with 40 mg of porous barium titanate to...

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Abstract

The invention discloses a piezoelectric support and its preparation method and application. The preparation method of the piezoelectric support includes decellularization of bone tissues and organs, and then supercritical carbon dioxide extraction to obtain a decellularized bone tissue with a three-dimensional porous structure. matrix; preparing drug-loaded microspheres; then mixing the drug-loaded microspheres with the decellularized matrix, so that the drug-loaded microspheres are covered on the surface of the decellularized matrix and filled in the pores of the decellularized matrix, at 37-65°C Insulation under conditions until the drug-loaded microspheres are bonded to the acellular matrix to prepare a scaffold containing the drug-loaded microspheres; then soak the drug-loaded microspheres in the degradable piezoelectric polymer solution, take it out and drain, Perform polarization treatment. The scaffold prepared by the preparation method of the piezoelectric scaffold of the present invention can effectively delay the release rate of drugs, has osteogenic differentiation ability and piezoelectric response function, and can effectively promote the repair and reconstruction of bone tissue.

Description

technical field [0001] The invention relates to the technical fields of biomedical engineering and biomedical materials, in particular to a piezoelectric support and a preparation method and application thereof. Background technique [0002] With the continuous development of medicine, pharmacy, biology and other disciplines, new drugs for various diseases emerge in an endless stream, but how to make the drug release continuously and stably in the body is still a difficult problem. Whether it is oral administration or intravenous injection, there will be a "peak and valley" phenomenon in the change of blood drug concentration. Too high a drug concentration will lead to greater toxic and side effects, while too low a therapeutic effect will not be achieved. Although emerging bioactive macromolecular drugs are highly efficient, they have short biological half-lives and are easily inactivated, which is also a problem that plagues drug developers. The drug is embedded or adsorb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/34A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/3633A61L27/365A61L27/34A61L27/50A61L27/56A61L27/54A61L27/58A61L2430/02A61L2300/62A61L2300/602A61L2300/414A61L2300/412A61L2300/216C08L67/04
Inventor 许为康李桂香周新婷黄德群
Owner GUANGDONG PROV MEDICAL INSTR INST
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