Automatic virus treatment method

[0032] The specific implementation of the automatic virus processing method of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0033] See attached figure 1 , the virus automatic processing method is realized by using a virus automatic processing system, and the virus automatic processing system includes a biological safety cabinet, a virus pretreatment unit, a nucleic acid purifier, a system construction unit, a PCR instrument and a control unit, and each unit and equipment complete each stage-specific features. The biological safety cabinet is used for the safety guarantee function of virus pretreatment. The virus pretreatment unit completes sample separation, freezing and inactivation, the nucleic acid purifier performs nucleic acid purification, and the body system construction unit performs mixed liquid treatment on the purified sample, and then The PCR instrument performs amplification and completes the detection. Equipped with intelligent operation of robots, each unit and equipment are connected in series into an organic whole, and the implementation of the entire process is completed by the control unit.

[0034] Specific steps are as follows:

[0035] (1) Arrange the samples and consumables in the virus pretreatment unit in the biological safety cabinet. After the samples are pre-collected, the sample bottle array is arranged on the vibrating device. Consumables were arranged in the nucleic acid purifier and PCR instrument. The biosafety cabinet was Thermo Fisher’s 1379 model, the nucleic acid purifier was Roche’s MagNA Pure96 model, and the PCR instrument was Roche’s LightCycler 480 II model. The corresponding consumables can be configured according to the specific product, and the product manual is strictly followed. Ensure the safety of all equipment and make other corresponding work preparations.

[0036] (2) Oscillate the sample bottle in the virus pretreatment unit. The oscillation process is completed on the oscillation device. Set the frequency and time of oscillation according to the source and type of the sample collected, so that the effective substances in the sample can be fully mixed and dissolved in the sample. solution in the bottle.

[0037] (3) Uncap the sample vials to be processed and three cryopreservation tubes. The cryopreservation tubes are pre-arranged in the virus pretreatment unit, and the three cryopreservation tubes are uncapped each time through the mechanical arm and the arranged turntable unit. action.

[0038] (4) Quantitatively transfer the sample solution in the sample vial to three cryotubes. The turntable unit is used for uncapping at one station and pipetting at one station. During the pipetting process, the pipette head should be replaced according to the process requirements.

[0039] (5) After the pipetting is completed, close the caps of the three cryotubes in turn, return the two cryotubes through the robotic arm, and send one to the metal bath.

[0040] (6) Repeat steps (3)-(5) to complete the pipetting for all the scheduled cryovials. Before opening the sample bottle every time when pipetting liquid, scan the code of the sample bottle and transmit the data to the background database. The pipetted vials are capped and returned to their original positions.

[0041] (7) After the metal bath inactivation of the cryopreservation tube is completed, open the cap and distribute the inactivated samples to the orifice plate; during the inactivation process, the metal bath inactivation temperature is 56°C and the time is 30 minutes. Similarly, during the pipetting process, the pipetting head should be replaced according to the process.

[0042] (8) After the inactivation is completed, uncap the inactivated cryotube on the turntable unit, transfer the inactivated sample to the orifice plate output, and send it to the nucleic acid purifier for purification. At the same time, the configuration of the mixed solution is carried out in advance for the construction of the PCR body system.

[0043] (9) After the purification is completed, it is sent to the construction of the PCR system, and the configuration of the mixed solution is completed at this time for the construction of the PCR system.

[0044] (10) PCR system construction The mixed solution and the inactivated sample are configured to generate a PCR reaction plate, which is packaged and exported. The PCR reaction plate is filled with inactivated samples and mixed solutions, and the liquid transfer is realized through the liquid injection gun. The transfer process follows the principle of non-intersection between samples to prepare for the replacement of pipette tips.

[0045] (11) The PCR reaction plate is centrifuged by a centrifuge, so that the droplets in the PCR reaction plate are disposed of at the bottom of the orifice plate. During centrifugation, a group of two plates is centrifuged.

[0046] (12) Send the PCR reaction plate to the PCR instrument for amplification and complete the detection. After completion, all consumables and discarded parts are recycled, and the data link data is completed.

[0047] The transfer of containers between biological safety cabinets, virus pretreatment units, nucleic acid purification instruments, system construction units, and PCR instruments is completed by robots. The lower computer of each device is connected to the upper computer of the console for control. Since biological safety cabinets, nucleic acid purifiers, and PCR instruments are existing outsourced equipment, the system access cannot be realized through the lower computer, and the robot is used to transmit instructions to the equipment through the hard interface, that is, by simulating manual operation, the keys of the equipment are controlled. The operation implements the control of the device.