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Nucleic acid purification method and nucleic acid purification equipment

A purification method and nucleic acid technology, which can be used in biochemical equipment and methods, DNA preparation, and microbial determination/inspection, etc., can solve the problems of inability to meet the requirements of rapid nucleic acid extraction and detection, insufficient pathogen lysis efficiency, and long extraction time. High-efficiency purification, large-scale automated extraction, and high extraction efficiency

Active Publication Date: 2021-03-16
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some problems in the current magnetic bead nucleic acid purification technology. For example, when using Thermo Fisher Kingfisher products to extract nucleic acids, the entire extraction and purification time is more than 20 minutes, and the extraction time is long, which cannot meet the rapid extraction of nucleic acids during mass disease outbreaks. testing needs
[0005] In addition, in the process of magnetic bead nucleic acid extraction, pathogen lysis is also very important, and it is a key factor affecting the extraction rate of nucleic acid. However, the efficiency of pathogen lysis is still insufficient and needs to be improved.

Method used

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  • Nucleic acid purification method and nucleic acid purification equipment
  • Nucleic acid purification method and nucleic acid purification equipment
  • Nucleic acid purification method and nucleic acid purification equipment

Examples

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Effect test

Embodiment 1

[0091] a Use the hepatitis B virus (HBV) nucleic acid quantitative enterprise reference substance calibrated by the China Institute for Biological Products as the sample to be tested, mix the virus sample, lysate and magnetic beads, and absorb the magnetic beads and mix with the virus sample, lysate The liquid mixing took 15s to obtain 700 μL of mixed solution, insert the vibrating rod to vibrate (the vibrating rod is placed vertically, and the vibration direction is the vertical direction), and vibrate at a frequency of 7 Hz and an amplitude of 8 mm to obtain a solution after lysis. Magnetic beads and nucleic acids are in Binding under shaking conditions, the binding time is 2min; among them, the magnetic beads are YL024 silanol magnetic beads provided by Ferrotec Group; the lysis solution includes 40% (w / v) guanidine hydrochloride, 10% (w / v) isothiocyanate Guanidine acid, 5% (v / v) polyethylene glycol p-isooctyl phenyl ether, and 5% (v / v) sodium lauroyl sarcosinate.

[0092] ...

Embodiment 2

[0110] The difference from Example 1 is that in step a, the standard product of the China Institute for the Control of Biological Products Neisseria gonorrhoeae is used as the sample to be tested.

Embodiment 3

[0112] The difference from Example 1 is that in step a, Ureaplasma urealyticum, a standard product of the Chinese Institute for the Control of Biological Products, is used as the sample to be tested.

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Abstract

The invention provides a nucleic acid purification method and nucleic acid purification equipment, and relates to the technical field of biology. The nucleic acid purification method provided by the invention comprises the following steps: firstly, mixing a sample with a lysis solution and magnetic beads, so that nucleic acid after cell lysis is fully adsorbed by the magnetic beads; and then, intermittently moving a magnetic bead collecting device to the bottom from the liquid level of a cracked solution, so that the operation mode of the magnetic bead collecting device cooperates with the sedimentation of the magnetic beads, the magnetic beads are fully adsorbed in an extremely short time, and the high-efficiency purification of the nucleic acid is further realized. The purification method disclosed by the invention is simple, quick and efficient, can finish extraction and purification of the nucleic acid within 8 minutes, and is high in extraction efficiency.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a nucleic acid purification method and nucleic acid purification equipment. Background technique [0002] Nucleic acid is the carrier of genetic information, the most important biological information molecule, and the main object of molecular biology research. Therefore, nucleic acid extraction is the most important and basic technology in molecular biology experimental technology. [0003] The current nucleic acid extraction methods include phenol-chloroform extraction, salting out, silica gel column purification, magnetic bead method, etc. Among them, magnetic bead nucleic acid purification technology is a relatively common nucleic acid extraction method. Under the action of an external magnetic field, magnetism can specifically recognize and efficiently bind to nucleic acid molecules, thereby realizing the separation of DNA and RNA in blood, animal tissue, feces, pathogeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2523/308C12Q2527/125
Inventor 王虹军陈芝娟王翔张磊建贺贤汉
Owner HANGZHOU BIOER TECH CO LTD