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Nucleic acid purification method and nucleic acid purification equipment

A purification method and nucleic acid technology, applied in biochemical equipment and methods, DNA preparation, microbial measurement/inspection, etc., can solve the problems of long extraction time, inability to meet the rapid extraction and detection of nucleic acid, and insufficient efficiency of pathogen lysis, etc., to achieve extraction High efficiency, large-scale automatic extraction, simple purification method

Active Publication Date: 2021-05-07
HANGZHOU BIOER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some problems in the current magnetic bead nucleic acid purification technology. For example, when using Thermo Fisher Kingfisher products to extract nucleic acids, the entire extraction and purification time is more than 20 minutes, and the extraction time is long, which cannot meet the rapid extraction of nucleic acids during mass disease outbreaks. testing needs
[0005] In addition, in the process of magnetic bead nucleic acid extraction, pathogen lysis is also very important, and it is a key factor affecting the extraction rate of nucleic acid. However, the efficiency of pathogen lysis is still insufficient and needs to be improved.

Method used

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  • Nucleic acid purification method and nucleic acid purification equipment
  • Nucleic acid purification method and nucleic acid purification equipment
  • Nucleic acid purification method and nucleic acid purification equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] a Use the Hepatitis B virus (HBV) nucleic acid quantitative enterprise reference product calibrated by the China Institute for the Control of Biological Products as the sample to be tested, mix the virus sample, lysate and magnetic beads, draw the magnetic beads and mix with the virus sample, lyse The solution was mixed for 15 seconds to obtain 700 μL of the mixed solution. Insert a vibrating rod for shaking (place the vibrating rod vertically, and the vibration direction is the vertical direction), and vibrate at a frequency of 7 Hz and an amplitude of 8 mm to obtain a lysed solution. Binding under shaking conditions, the binding time is 2min; wherein, the magnetic beads are YL024 silanol magnetic beads provided by Ferrotec Group; the lysate includes 40% (w / v) guanidine hydrochloride, 10% (w / v) isothiocyanate Guanidine acid, 5% (v / v) polyethylene glycol p-isooctyl phenyl ether and 5% (v / v) sodium lauroyl sarcosinate.

[0092] b Adsorb the magnetic beads by intermittent...

Embodiment 2

[0110] The difference from Example 1 is that in step a, the standard product Neisseria gonorrhoeae of China Institute for the Control of Biological Products is used as the sample to be tested.

Embodiment 3

[0112] The difference from Example 1 is that in step a, the standard product Ureaplasma urealyticum of China Institute for the Control of Biological Products is used as the sample to be tested.

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Abstract

The invention provides a nucleic acid purification method and nucleic acid purification equipment, which relate to the field of biotechnology. In the nucleic acid purification method provided by the present invention, firstly, the sample, lysate and magnetic beads are mixed, so that the nucleic acid after cell lysis is fully adsorbed by the magnetic beads; The operation mode of the magnetic bead collection device cooperates with the sedimentation of the magnetic beads to achieve sufficient adsorption of the magnetic beads in a very short time, thereby achieving high-efficiency purification of nucleic acids. The purification method of the present invention is simple, quick and efficient, can complete the extraction and purification of nucleic acid within 8 minutes, and has high extraction efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid purification method and nucleic acid purification equipment. Background technique [0002] Nucleic acid is the carrier of genetic information, the most important biological information molecule, and the main object of molecular biology research. Therefore, the extraction of nucleic acid is the most important and basic technology in molecular biology experimental techniques. [0003] At present, nucleic acid extraction methods include phenol chloroform extraction, salting-out method, silica gel column purification, magnetic bead method, etc. Among them, the magnetic bead nucleic acid purification technology is a relatively common method for nucleic acid extraction. Magnetism, under the action of an external magnetic field, can specifically recognize and efficiently combine with nucleic acid molecules, thereby realizing the separation of DNA and RNA in samples such as bl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2523/308C12Q2527/125
Inventor 王虹军陈芝娟王翔张磊建贺贤汉
Owner HANGZHOU BIOER TECH CO LTD