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Achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop

A technology of p-toluenesulfonic acid and achromobacter, which is applied in the biological field and can solve problems such as human and animal safety impacts, pollution, and ecological damage

Active Publication Date: 2022-07-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although quizalofop-p-ethyl is considered to be a low-toxicity pesticide, its widespread use and a large amount of residue and accumulation in the environment have caused global environmental pollution and ecological damage in addition to directly affecting the soil environment, water environment and atmospheric environment. In addition to causing pollution, quizalofop-ethyl will also accumulate in organisms through the food chain, which will have a serious impact on the safety of humans and animals

Method used

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  • Achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop
  • Achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop
  • Achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation of Achromobacter sp MBZ-2

[0032] Sludge samples were collected from the activated sludge tank of a wastewater treatment plant of a pesticide production enterprise in Jiangsu, and the continuous enrichment culture method was used for enrichment culture. The degradation effect was tested by high performance liquid chromatography. 0.5g / L solid inorganic salt of p-toluenesulfonic acid (medium formula: p-toluenesulfonic acid 0.5g / L, NH 4 Cl 1.0g / L, KH 2 PO 4 0.6g / L, K 2 HPO 4 1.3g / L, NaCl 0.5g / L, MgSO 4 ·7H 2 0.2 g / L, pH 7.0. ) medium, cultured in a constant temperature incubator at 30°C until colonies grew. According to the size, color and shape of the colony, pick different single colonies for streak purification. The strain whose degradation efficiency was 98.4% after 72h was named as MBZ-2.

Embodiment 2

[0033] Example 2: Determination of the degradation effect of Achromobacter sp MBZ-2 on quizalofop

[0034] Achromobacter sp MBZ-2 was streaked on the LB solid plate, and then a single colony was picked from the LB plate, inoculated into the LB liquid medium, and cultivated at 37°C, 180rpm in a shaker to OD600= After centrifugation at 1.0, 10,000 rpm, the supernatant was discarded, washed three times with sterile water, and resuspended in an equal volume of sterile water as a seed solution. The MBZ-2 seed liquid was inoculated in the inorganic salt medium with quizalofop-p-ethyl concentration of 50 mg / L at an inoculum of 1vt%, cultivated in a shaker at 37°C and 180 rpm, and the quinazoline in the solution was measured after sampling and filtration every 4h. Heling concentration, the effect is as follows image 3 shown, the degradation curve see Figure 4 .

Embodiment 3

[0035] Example 3: Determination of the degradation effect of Achromobacter sp MBZ-2 on p-toluenesulfonic acid

[0036] Achromobacter sp MBZ-2 was streaked on the LB solid plate, and then a single colony was picked from the LB plate, inoculated into the LB liquid medium, and cultivated at 37°C, 180rpm in a shaker to OD600= After centrifugation at 1.0, 10,000 rpm, the supernatant was discarded, washed three times with sterile water, and resuspended in an equal volume of sterile water as a seed solution. The MBZ-2 seed solution was inoculated into an inorganic salt medium with a concentration of 500 mg / L of p-toluenesulfonic acid at an inoculum amount of 1 vt%, and cultured at 37 °C in a 180 rpm shaker. p-toluenesulfonic acid concentration, the effect is as figure 2 shown, the degradation curve see Figure 5 .

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Abstract

The present invention discloses a strain of Achromobacter sp, which is classified and named as Achromobacter sp, and the strain is named as MBZ-2, which has been preserved in the General Microbiology Center of the China Committee for the Preservation of Microorganisms with a preservation date of August 2020 On the 13th, the deposit number was CGMCC No.20529. The Achromobacter provided by the invention can effectively degrade p-toluenesulfonic acid and its downstream product herbicide quizalofop-p-ethyl.

Description

technical field [0001] The invention relates to the biological field, in particular to an achromobacter and its application in degrading p-toluenesulfonic acid and quizalofop-p-ethyl. Background technique [0002] p-toluenesulfonic acid (p-toluenesulfonic acid, referred to as PTS), its molecular formula is: C 7 H 8 O 3 S, the structural formula is shown in formula I. p-Toluene sulfonic acid is white needle or powder crystal, melting point 106-107℃, boiling point 140℃ / 2.67kPa, easily soluble in polar solvents such as water, alcohol, ether, etc., insoluble in non-polar solvents such as benzene and toluene. It is a non-oxidizing organic strong acid, and its acidity is 1 million times that of benzoic acid. It is an important chemical synthesis raw material and is widely used as an intermediate for synthetic medicines (such as naproxen, doxycycline, etc.), pesticides (such as quizalofop-p-ethyl, dicofol, etc.) and dyes (acid orange 7, etc.) It is also used as a catalyst for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/36C02F101/30
CPCC12N1/20C02F3/34C02F2101/40C02F2101/36C02F2101/306
Inventor 蔡天明蔡舒陈立伟
Owner NANJING AGRICULTURAL UNIVERSITY
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