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Magnetic suspension gene biological imaging micro-fluidic chip switching device and imaging method thereof

A microfluidic chip and bio-imaging technology, applied in biochemical equipment and methods, measuring devices, microbial measurement/inspection, etc., can solve problems such as slow imaging speed, improve imaging accuracy, increase imaging speed, and avoid impact Effect

Pending Publication Date: 2021-03-23
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology

[0003] In order to solve the problems existing in the prior art, the present invention provides a magnetic levitation gene biological imaging microfluidic chip switching device and its imaging method, which solves the technical defect of slow imaging speed in the prior art

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  • Magnetic suspension gene biological imaging micro-fluidic chip switching device and imaging method thereof
  • Magnetic suspension gene biological imaging micro-fluidic chip switching device and imaging method thereof
  • Magnetic suspension gene biological imaging micro-fluidic chip switching device and imaging method thereof

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Embodiment Construction

[0025] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0026] Such as figure 1 As shown, the magnetic levitation gene bioimaging microfluidic chip switching device includes: an area array CCD sensor 1, a filter I2, a lens I3, a lens II4, a microscope housing 5, a Y-direction scanning vibrating mirror 6, a laser light source 7, Filter II 8, microscope eyepiece 9, microscope objective lens 10, scanning galvanometer in X direction 11, fixing nut 12, magnetic material 13, baffle plate 14, gene chip switching platform 15, magnetic levitation base 16, leveling bubble 17, Electromagnet 18, connecting column 19, controller 20, power amplifier 21, power supply 22. The filter I2, lens I3, lens II4, Y-direction scanning vibrating mirror 6, laser light source 7, optical filter II8, microscope eyepiece 9, microscopic objective lens 10, and X-direction scanning vibrating mirror 11 are placed in the microscope ...

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Abstract

The invention discloses a magnetic suspension gene biological imaging micro-fluidic chip switching device and an imaging method thereof, and relates to the field of fluorescence microscope instrumentdesign and preparation. The device comprises an exciting light module, a detection module, a magnetic suspension circuit and a magnetic suspension micro-fluidic chip switching device; a magnetic suspension circuit provides buoyancy for the magnetic suspension micro-fluidic chip switching device, enables a gene chip to move in the X direction, the Y direction and the Z direction on the magnetic suspension micro-fluidic chip switching device and prevents ground vibration from influencing imaging; the exciting light module is used for generating two paths of laser with different wavelengths to pass through a laser collimating and beam expanding system; and the detection module is used for irradiating the collimated and expanded laser emitted by the exciting light module to the surface of a sample to cause a fluorescence effect and carrying out signal acquisition imaging on fluorescence generated by the sample. The invention provides a new detection device for biological gene imaging, andmeanwhile, the device has the advantages of high resolution, miniaturization, fast acquisition and imaging and the like.

Description

technical field [0001] The invention belongs to the field of design and preparation of fluorescent microscope instruments, and in particular relates to a magnetic levitation gene biological imaging microfluidic chip switching device and an imaging method thereof. Background technique [0002] In the field of biomedicine, fluorescence microscopy imaging technology has been widely recognized and applied as an essential observation technology. At present, the commonly used confocal microscope is a line-scanning confocal microscope. The line-scanning uses a line array detector instead of the traditional point imaging detector, and finally changes the two-dimensional point scanning imaging into a one-dimensional line scanning imaging, which improves the imaging speed. and resolution. But the imaging speed is still slow. Contents of the invention [0003] In order to solve the problems existing in the prior art, the present invention provides a magnetic levitation gene biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01C12Q1/68
CPCG01N21/6402G01N21/6458G01N21/6454G01N21/01C12Q1/68C12Q2563/107C12Q2565/601C12Q2565/607
Inventor 陈启梦刘子琦李子博王哲
Owner CHANGCHUN UNIV OF SCI & TECH
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