Application of melaleuca bracteata in acaricidal and insecticidal aspects, biological prevention and control medicament and preparation method thereof
A kind of technology of Dahei medicine, which is applied in the field of biological control, can solve the unreported application of Dahei medicine to kill mites and insects, etc.
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preparation example Construction
[0052]The embodiment of this group provides a preparation method of biological control agent. All the examples in this group have the following common features: the preparation method includes: boiling or distilling, condensing and extracting Dahei medicine.
[0053] In a specific embodiment, the distillation refers to steam distillation for 8-12 hours, preferably 10 hours, to fully extract the volatile components in the plant material.
[0054] The poaching refers to boiling with water for 8-12 hours, preferably 10 hours, so that the essential oil components can be boiled out of the plants as completely as possible.
[0055] Preferably, the extraction refers to extraction with n-hexane;
[0056] More specifically, a 2L separatory funnel was used, n-hexane:distillate=1:1, and the n-hexane layer in which the essential oil had been dissolved was retained after layering.
[0057] Preferably, the temperature of the water bath for the extraction is 38-45°C, preferably 40°C. At th...
experiment example 1
[0062] Experimental example 1, preparation of Daheiyao essential oil
[0063] 3kg Dahei medicine was purchased from Yunyao.com, and treated with water for 8h-10h. The obtained oil-water mixture was condensed and recovered, then extracted with n-hexane, dried with anhydrous sodium sulfate, and the n-hexane was removed by rotary evaporator. Black medicine essential oil. Wherein, the temperature of the water bath is 40°C.
[0064] The yield of Daheiyao essential oil is 0.60% (w / w), that is, 0.6g of Daheiyao essential oil can be extracted from 100g of Daheiyao.
experiment example 2
[0065] Experimental example 2. Identification of essential oil components of Daheiyao
[0066] GC-MS detection method was used to identify the components of Daheiyao essential oil:
[0067] Detection instruments: gas chromatograph (Agilent6890N) and mass spectrometer (Agilent5973N). .
[0068] Detection conditions: HP-5MS 30mm×0.25mm×0.25μm quartz capillary column, the initial column temperature is 60°C, keep it for 1min, at the rate of 12°C / min, program the temperature to 180°C and keep it for 0.5min, then at the rate of 25°C / min The temperature was programmed to 280°C for 18 minutes, the vaporizer temperature was 280°C, the carrier gas was helium, the column flow rate was 1.0mL / min, and the split ratio was 1:10.
[0069] The mass spectrometer ionization mode is EI, the electron bombardment energy is 70eV, the scanning mass range is 20-550m / z, and the scanning time is 2s.
[0070] Take 1 μL (100 times acetone dilution) of Daheiyao essential oil as a sample, analyze and ide...
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