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A kind of Flammulina velutipes strain and its identification and breeding method

An identification method, the technology of Flammulina velutipes, applied in the field of edible fungi, can solve the problems of fast and accurate identification of mononuclear, dinuclear and hybrid strains of Flammulina velutipes, and achieve bright colors, high biological efficiency, and good palatability

Active Publication Date: 2022-07-22
河北省农林科学院经济作物研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome defects such as the mononucleus, dinucleus and hybrid strains of Flammulina velutipes that can not be identified quickly and accurately in the prior art, thereby providing a kind of Flammulina velutipes bacterial strain and its identification and breeding method

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  • A kind of Flammulina velutipes strain and its identification and breeding method
  • A kind of Flammulina velutipes strain and its identification and breeding method
  • A kind of Flammulina velutipes strain and its identification and breeding method

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Embodiment 1

[0050] A strain of Flammulina velutipes, preserved in the General Microbiology Center of China Microbial Culture Collection Management Committee, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, preservation number: CGMCCNo.19644, preservation date: May 2020 On March 26, it was named Jijin 12; classification name: Flammulinafiliformis.

[0051] A method for identifying Flammulina velutipes strains, the two cell nuclei of the double monohybrid are one of the acceptor nucleus derived from the mononuclear parent, and the other one of the donor nucleus derived from the dinuclear parent. The parents of Jijin 12 include monokaryotic strain parents and dikaryotic strain parents. The monokaryotic strain parent is obtained by the preparation and regeneration of protoplast monokaryon of Flammulina velutipes strain HUANG1, and the dikaryotic parent is the fruit body of wild Flammulina velutipes strain JHH by tissu...

Embodiment 2

[0073] The breeding method of above-mentioned Flammulina velutipes strain, comprises the steps:

[0074] Take the mycelium of the yellow cultivated Flammulina velutipes strain HUANG1 and inoculate it in a conical flask filled with a liquid medium, culture at 25°C for 8 days, shake the conical flask by hand every 24h, and collect the conical flasks in the liquid medium under aseptic conditions. Mycelium, with 2% lysozyme enzyme solution (purchased from Guangdong Institute of Microbiology), enzymatically hydrolyzed at 30 ° C for 4 hours, filtered off the broken mycelium, washed 3 times with 0.6M sucrose solution, and purified The protoplast suspension was diluted with 0.6M sucrose solution to a concentration of 300 / ml, and 0.2ml was drawn and spread on the regeneration medium, cultured at 25°C for 8 days, and a single colony was picked and stored on the slant of the test tube. Cultivate at 25°C for 4 days, take the mycelium slices, and observe under the microscope whether there ...

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Abstract

The invention belongs to the technical field of edible fungi, in particular to a method for breeding and identification of Flammulina velutipes. The strain selection method includes carrying out double monohybrid with the protoplast monokaryotic strain of the cultivated strain as the monokaryotic parent and the wild strain as the binuclear parent, identifying the authenticity of the hybrid, and further determining the mycelial stage and the fruiting stage of the hybrid. Perform screening to obtain excellent hybrids, and finally perform single spore selfing, tissue isolation and fruiting screening on the screened hybrids to obtain excellent Flammulina velutipes strains. The identification methods of hybrid strains include the extraction of DNA after hyphal culture of Flammulina velutipes parental strains and their protoplast monokaryotic strains, ITS sequence amplification and sequencing, SNP site analysis, specific primer design and screening, and identification of parental strains and hybrid strains. AS-PCR amplification and electrophoresis detection; the cap of this strain is golden yellow with bright color, the stipe is slender, the color of the stipe is yellow-white and not easy to brown, and the base of the stipe has few villi and no adhesion.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a Flammulina velutipes strain and a method for identification and breeding thereof. Background technique [0002] Foliformis (Flammulina filiformis) belongs to the genus Foliformis, and its Chinese name is Flammulina velutiper. The Latin name of F. velutiper (Fr) Sing has been used until 2018, when Yang Zhuliang and his team from the Kunming Institute of Botany, Chinese Academy of Sciences conducted a study on Chinese Flammulina. To correct the name, research has proved that my country's Flammulina velutiper is different from F. velutiper in Europe and the United States. It is a new species, and the Latin name of F. filiformis should be adopted. Flammulina velutipes are rich in a variety of nutrients, and are also known as "educational mushrooms" because they are rich in "lysine". Flammulina velutipes is the fastest growing edible mushroom species in China. At pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/04C12Q1/6858C12Q1/6895C12Q1/04A01G18/00C12R1/645
CPCC12N15/04C12Q1/6858C12Q1/6895A01G18/00C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 李慧高春燕王朝江夏伟伟董庆武
Owner 河北省农林科学院经济作物研究所