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Culture medium additive for improving content of organic selenium in selenium yeast

A medium additive and selenium yeast technology, applied in the field of selenium yeast, can solve the problem of low organic selenium content in selenium yeast, and achieve the effects of reducing peroxidative damage, high efficiency and easy preparation

Active Publication Date: 2021-03-26
安徽省华信生物药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the content of organic selenium in the selenium yeast produced by the existing fermentation culture method is not high, the invention provides a medium additive for increasing the content of organic selenium in the selenium yeast

Method used

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  • Culture medium additive for improving content of organic selenium in selenium yeast
  • Culture medium additive for improving content of organic selenium in selenium yeast
  • Culture medium additive for improving content of organic selenium in selenium yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of culture medium additive preparation operation that improves organic selenium content in selenium yeast is as follows:

[0036] (1) 1000mg / L reduced glutathione (GSH), 100mg / L reduced nicotinamide adenine dinucleotide phosphate, 40g / L fructose and 400mg / L adenine nucleoside triphosphate (ATP) were respectively Dissolve thoroughly with a small amount of distilled water, mix the four substances evenly, and dilute to a 1L aqueous solution.

[0037] (2) Sterilization

[0038] The aqueous solution is taken, sterilized by a filter sterilizer with a filter membrane pore size of 0.22 micron, and stored in a reagent bottle to obtain a medium additive for increasing the content of organic selenium in selenium yeast.

[0039] The using method of described culture medium additive is as follows:

[0040] The activated yeast was transferred to 1 L of fresh liquid medium according to the inoculum amount of 10%, under the condition of temperature 28°C and shaking speed of 18...

Embodiment 2

[0043] A kind of culture medium additive preparation operation that improves organic selenium content in selenium yeast is as follows:

[0044] (1) 1000mg / L reduced glutathione (GSH), 100mg / L reduced nicotinamide adenine dinucleotide phosphate, 40g / L fructose and 800mg / L adenine nucleoside triphosphate (ATP) were respectively Dissolve thoroughly with a small amount of distilled water, mix the four substances evenly, and dilute to a 1L aqueous solution.

[0045] (2) Sterilization

[0046] The aqueous solution is taken, sterilized by a filter sterilizer with a filter membrane pore size of 0.22 micron, and stored in a reagent bottle to obtain a medium additive for increasing the content of organic selenium in selenium yeast.

[0047] The using method of described culture medium additive is as follows:

[0048] Transfer the activated yeast to 1 L of fresh liquid medium according to the inoculum amount of 10%, and culture it in a shaker flask for 6 hours at a temperature of 28°C ...

Embodiment 3

[0051] A kind of culture medium additive preparation operation that improves organic selenium content in selenium yeast is as follows:

[0052] (1) 1000mg / L reduced glutathione (GSH), 100mg / L reduced nicotinamide adenine dinucleotide phosphate, 40g / L fructose and 600mg / L adenine nucleoside triphosphate (ATP) were respectively Dissolve thoroughly with a small amount of distilled water, mix the four substances evenly, and dilute to a 1L aqueous solution.

[0053] (2) Sterilization

[0054] The aqueous solution is taken, sterilized by a filter sterilizer with a filter membrane pore size of 0.22 micron, and stored in a reagent bottle to obtain a medium additive for increasing the content of organic selenium in selenium yeast.

[0055] The using method of described culture medium additive is as follows:

[0056] The activated yeast was transferred to 1 L of fresh liquid culture medium according to the inoculum amount of 10%, under the conditions of temperature 28°C and shaking sp...

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Abstract

The invention discloses a culture medium additive for improving the content of organic selenium in selenium yeast, and belongs to the technical field of selenium yeast. The culture medium additive consists of the following components: 1000 mg / L of reduced glutathione (GSH), 100 mg / L of reduced nicotinamide adenine dinucleotide tetrasodium phosphate (NADPH-Na4), 40 g / L of fructose and 400-800 mg / Lof ATP. Orthogonal experiments are carried out on the addition amounts of the four culture medium additives, and through the experiments, it finds that the selenium-enriched yeast obtained by adding the additive into the culture medium has the characteristics of high biomass, high selenium enrichment, high organic selenium conversion, high yield and the like, and the problem that the conversion amount of organic selenium in the selenium-enriched yeast is small is effectively solved.

Description

technical field [0001] The invention belongs to the technical field of selenium yeast, and in particular relates to a medium additive for increasing the content of organic selenium in selenium yeast. Background technique [0002] Selenium (Se) is an essential trace element for the human body. It is necessary for many proteins in the body to perform physiological functions such as maintaining the homeostasis of the immune system and delaying aging. Supplementing an appropriate amount of selenium every day is very important for the health of the body. It can not only prevent Keshan disease, Kashin-Beck disease, and can greatly reduce the risk of cardiovascular system, endocrine system, nervous system, reproductive system, sports system and other diseases. [0003] Selenium is divided into two forms: inorganic selenium and organic selenium. The common inorganic selenium is sodium selenate and sodium selenite. Due to the low bioavailability of inorganic selenium and high toxicit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/38
CPCC12N1/16C12N1/38
Inventor 张玉英杨思林魏春厅
Owner 安徽省华信生物药业股份有限公司
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