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Compound probiotic preparation for preventing and treating piglet diarrhea and preparation method and application

A technology of compound probiotics and piglet diarrhea, which is applied in the direction of antibacterial drugs, drug combinations, pharmaceutical formulas, etc., can solve problems such as difficulty in achieving the expected effect, uneven antibacterial effect, lack of good probiotics, etc., and achieve improvement The effect of improving the intestinal micro-ecological environment of the body, improving growth performance, and avoiding bacterial resistance

Active Publication Date: 2021-04-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there have been related studies on the use of probiotics as feed additives to reduce the diarrhea rate of piglets. Due to some unfavorable factors such as probiotics, the antibacterial effect is uneven; gastrointestinal fluid intolerance; bile intolerance; antibiotic intolerance Problems such as probiotics often have good effects under laboratory conditions, but it is often difficult to achieve the desired effect under production conditions
At the same time, there is a serious shortage of excellent probiotic strains that can be used to inhibit pathogenic bacteria to prevent or treat piglet diarrhea, and there are fewer strains that can be used in microecological preparations, and related products are relatively single

Method used

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  • Compound probiotic preparation for preventing and treating piglet diarrhea and preparation method and application
  • Compound probiotic preparation for preventing and treating piglet diarrhea and preparation method and application
  • Compound probiotic preparation for preventing and treating piglet diarrhea and preparation method and application

Examples

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Effect test

Embodiment 1

[0032] A compound probiotic preparation for resisting pathogenic Escherichia coli infection, including Clostridium butyricum, Lactobacillus reuteri, and Bifidobacterium longum, the preparation method of which is as follows:

[0033] Three strains of Clostridium butyricum, Lactobacillus reuteri, and Bifidobacterium longum were respectively inoculated into fresh liquid medium for cultivation to obtain three bacterial suspensions;

[0034] Centrifuge the prepared three bacterial suspensions to obtain bacterial pellets, and resuspend the bacterial pellets with fresh medium;

[0035] Mix the three kinds of probiotic suspensions in proportion as a seed solution, and the total number of viable bacteria in each milliliter of compound probiotic preparation is 10 1 ~10 20 indivual;

[0036] Add 800g of sodium alginate, 200g of calcium carbonate, and 33L of distilled water into a 50L seed tank, mix well, and add compound probiotic seed liquid 10 after sterilization 5 ~10 15 cfu mL -...

Embodiment 2

[0040] The compound probiotic preparation of the anti-diarrhea Escherichia coli infection of piglets prepared in Example 1, its application process is as follows: all piglets are screened for enterotoxigenic E. The subcultures were carried out on plates, and if ETEC was found, it was eliminated in time.

[0041] 40 weaned piglets at the age of 21 days. The piglets were randomly divided into groups, Control group (I, 10 heads); Escherichia coli K88+0.9% Nacl group (II, 10 heads); Probiotics+Escherichia coli K88 group (III, 10 heads); Antibiotics+Escherichia coli K88 Group (IV, 10 heads), the experimental process is as follows figure 2 As shown, the specific test process:

[0042] 10mL of 0.9% Nacl; 10mL of 0.9% Nacl; 10mL of compound probiotic preparation; 10mL of 0.9% Nacl;

[0043] On the 8th day at 9 o'clock in the morning, 0.9% Nacl 10mL was fed respectively; E.coli K8810 10 cfu / mL 10mL; E.coli K8810 10 cfu / mL 10mL; E.coli K8810 10 cfu / mL 10mL, 12 hours later, the ex...

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Abstract

The invention discloses a compound probiotic preparation for preventing and treating piglet diarrhea and a preparation method and application. The compound probiotic preparation is prepared from clostridium butyricum, lactobacillus reuteri and bifidobacterium longum, wherein the total quantity of viable bacteria in the compound probiotic preparation is 10<1> to 10<20> per ml. The compound probiotic preparation disclosed by the invention has the advantages that the diarrhea rate and the death rate of weaned piglets are remarkably reduced. On the sixth to twentieth day of the age in days of the piglets, the prepared compound probiotic preparation is irrigated into stomachs of 66 large binary hybrid piglets, the irrigation dosage is 5 to 10ml / time, and the piglets are irrigated once every day. Compared with a control group irrigated by a Phosphate Buffer Solution (PBS), the compound probiotic preparation has the advantages that the diarrhea rate of the group irrigated by the compound probiotic preparation at the first to fifteenth day stage after weaning (21 days of age) is remarkably reduced by 87 percent, and the death rate is reduced by 70 percent. The compound probiotic preparation effectively prevents the piglet diarrhea.

Description

technical field [0001] The invention belongs to the technical field of compound probiotic preparations, and in particular relates to a compound probiotic preparation for preventing and treating piglet diarrhea, a preparation method and an application thereof. Background technique [0002] Piglets are the core of modern pig production, and their health status has an extremely important impact on later growth and development. In actual production, in order to maximize the economic benefits of pig raising, the technology of early weaning of piglets is widely used in intensive pig production at home and abroad. However, due to the incomplete development of the piglet's own immune system, environmental changes, dietary changes, and improper feeding methods during the early weaning period of piglets will weaken the piglet's immunity and reduce the level of intestinal antibodies, which is conducive to the pathogenic E. coli and other harmful microorganisms. Invasion causes diarrhe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K9/50A61K47/36A61P1/12A61P43/00A61P31/04A61P37/02A61P1/00A61K35/742A61K35/745
CPCA61K35/742A61K35/747A61K35/745A61K9/5036A61K9/5089A61P1/12A61P43/00A61P31/04A61P37/02A61P1/00A61K2300/00
Inventor 宋素泉闫丽萍丁晨晨
Owner NANJING AGRICULTURAL UNIVERSITY
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