Method for supplementing materials in batches to ferment coriolus versicolor by utilizing airlift fermentation tank

An air-lift fermenter, feeding technology, applied in the direction of microbial-based methods, biochemical equipment and methods, using added compounds to stimulate growth, etc., to achieve low production costs, low shear force, and beneficial to mycelium The effect of cultivation

Active Publication Date: 2021-04-09
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of the existing Yunzhi fungus liquid culture, the present invention provides a method for cultivating Yunzhi fungus by fed-batch fermentation in an air-lift fermenter

Method used

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  • Method for supplementing materials in batches to ferment coriolus versicolor by utilizing airlift fermentation tank
  • Method for supplementing materials in batches to ferment coriolus versicolor by utilizing airlift fermentation tank
  • Method for supplementing materials in batches to ferment coriolus versicolor by utilizing airlift fermentation tank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Cultivation of Yunzhi fungus by fed-batch fermentation in an air-lift fermenter.

[0025] (1) Seed liquid culture of Yunzhi fungus.

[0026] Versicolor versicolor grows rapidly in the seed tank (1#), the fermentation broth becomes viscous, and the mycelium of versicolor versicolor floccules to form bacterial balls, which are inoculated into the fermenter at a ratio of 10%.

[0027] The above seed medium is: soybean meal 1-2%, glucose 3-5%, peptone 0.3-0.5%, dipotassium hydrogen phosphate 01-0.2%, magnesium sulfate 0.05-0.1%, ammonium sulfate 0.1-0.2%, vitamin B10 .001-0.002%, defoamer 0.04-0.05%. (2) The seed solution was inoculated into the fermenter to culture Yunzhi bacteria in batch fed-batch fermentation.

[0028] Above-mentioned fermentor adopts air-lift fermenter (3#). The culture conditions are as follows: the ventilation ratio is 1:0.5-1.5vvm, the tank pressure is 0.02-0.05Mpa, and the fermentation culture is carried out at 28°C.

[0029] The above-mentione...

Embodiment 2

[0040] Batch fermentation of Yunzhi fungus in an air-lift fermenter.

[0041] All the other are identical with embodiment 1. The difference is that the fermentation process is batch fermentation, and no sugar is added during the fermentation process.

Embodiment 3

[0043] Batch fermentation and cultivation of Yunzhi fungus in mechanical stirring fermenter. All the other are identical with embodiment 1. The difference is that the fermenter adopts a mechanically agitated ventilated fermenter.

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Abstract

The invention provides a method for supplementing materials in batches to ferment coriolus versicolor by utilizing an airlift fermentation tank. The method comprises seed liquid culture of coriolus versicolor, and is characterized in that the seed liquid is inoculated into the airlift fermentation tank for fed-batch fermentation culture of coriolus versicolor, and then the coriolus versicolor are repeatedly fermented in batches; and the material supplementing refers to supplementing glucose, oligopeptide and hydrogen. The method can improve the production efficiency, improve the product quality and reduce the production cost.

Description

technical field [0001] The invention relates to the technical field of fungal culture, in particular to a method for fermenting versicolor versicolor in batches with an air-lift fermenter. Background technique [0002] Coriolus versicolor, also known as gray lucidum, Varrobacter, and Coriolus versicolor, is the fruiting body or mycelium of Versicolor versicolor, one of the fungi with the most medicinal value, and has the effects of clearing away heat and anti-tumor. Yunzhi is one of the fungi with the most medicinal value. It has the functions of clearing away heat, detoxification, anti-inflammatory, anti-cancer, and protecting the liver. The main active ingredient in Yunzhi is Yunzhi polysaccharide, which has immune regulation function and is a good immune enhancer, which can enhance the function and recognition ability of immune cells and increase the amount of IgM; Liver function, can significantly reduce serum transaminase, and has obvious repairing effect on liver tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38
Inventor 徐庆阳王英燕纪乐军范祎立曹宝臣
Owner JIANGSU SHENHUA PHARMA
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