Method for detecting concentration of DHA in blood in human body
A detection method and blood technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of flammability, affect accuracy, and difficult to control adsorption concentration, and achieve the effect of simple operation and satisfactory precision.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0020] S1. Blood sample pretreatment:
[0021] (1) Weigh 0.3 g of blood, put it into a 10 mL centrifuge tube, add 40 μL of C23:0 internal standard solution with a mass concentration of 40 μg / mL, 1.5 mL of distilled water, 400 μL of methanol solution containing 0.05% antioxidant (BHT), 2mL of chloroform, oscillate evenly, centrifuge at 2500r / min for 10min, take the chloroform layer, repeat the above extraction process, combine the chloroform layer into a 20mL headspace sampling bottle, blow dry with nitrogen for later use;
[0022] (2) Under the protection of nitrogen, add 200μLAMP to the above-mentioned dried headspace sample vial, fill it with nitrogen and seal it, react in a constant temperature oil bath at 180°C for 120min, then cool to room temperature, add methanol to dilute to 1mL, and the sample is passed through After filtering through a 0.22 μm filter membrane, it was analyzed and detected by LC-MS / MS.
[0023] S2. Preparation of standard stock solution and standard ...
Embodiment 2
[0030] S1. Blood sample pretreatment:
[0031] (1) Weigh 0.3 g of blood, put it into a 10 mL centrifuge tube, add 40 μL of C23:0 internal standard solution with a mass concentration of 40 μg / mL, 1.5 mL of distilled water, 400 μL of methanol solution containing 0.05% antioxidant (BHT), 2mL of chloroform, oscillate evenly, centrifuge at 2500r / min for 10min, take the chloroform layer, repeat the above extraction process, combine the chloroform layer into a 20mL headspace sampling bottle, blow dry with nitrogen for later use;
[0032] (2) Under nitrogen protection, add 200 μLAMP to the above dried headspace sample bottle, fill it with nitrogen and seal it, react in a constant temperature oil bath at 180 °C for 100 min, then cool to room temperature, add methanol to dilute to 1 mL, and the sample is passed through After filtering through a 0.22 μm filter membrane, it was analyzed and detected by LC-MS / MS.
[0033] S2. Preparation of standard stock solution and standard solution: ...
Embodiment 3
[0040] S1. Blood sample pretreatment:
[0041](1) Weigh 0.3 g of blood, put it into a 10 mL centrifuge tube, add 40 μL of C23:0 internal standard solution with a mass concentration of 40 μg / mL, 1.5 mL of distilled water, 400 μL of methanol solution containing 0.05% antioxidant (BHT), 2mL of chloroform, oscillate evenly, centrifuge at 2500r / min for 10min, take the chloroform layer, repeat the above extraction process, combine the chloroform layer into a 20mL headspace sampling bottle, blow dry with nitrogen for later use;
[0042] (2) Under nitrogen protection, add 200 μLAMP to the above dried headspace sampling vial, fill it with nitrogen and seal it, react in a constant temperature oil bath at 210 °C for 75 min, then cool to room temperature, add methanol to dilute to 1 mL, and the sample is passed through After filtering through a 0.22 μm filter membrane, it was analyzed and detected by LC-MS / MS.
[0043] S2. Preparation of standard stock solution and standard solution:
...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More