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Disinfection treatment method of sargassum thunbergii new branches

A treatment method, the technology of sargassum, applied in the direction of disinfectant, gardening method, botanical equipment and method, etc., can solve the unknown influence of sargassum growth, immature aseptic treatment technology, and difficulty in disinfection and treatment of sargassum And other issues

Active Publication Date: 2021-04-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with other large brown algae, Sargassum is more prone to epiphyte various marine bacteria, mussels, miscellaneous algae, etc. during the growth process, and there are also parasitic bacteria inside the algae, so it is very difficult to disinfect Sargassum, affecting The rapid development of sargassum research
Different algae positions of Sargassum have different tolerances to the type, concentration, and treatment time of disinfectants. Although some studies have carried out aseptic treatment experiments on Sargassum, the effects of various disinfectants on the growth of Sargassum are unknown.
At present, the aseptic treatment technology for the new shoots of Sargassum is not yet mature, so it is necessary to combine the disinfection effects of various disinfection methods and the impact on the growth of Sargassum to develop a disinfection treatment method suitable for the new shoots of Sargassum

Method used

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  • Disinfection treatment method of sargassum thunbergii new branches
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  • Disinfection treatment method of sargassum thunbergii new branches

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Disinfectant screening experiment: Sargassum was collected from Fujian (27°N, 121°E), the pre-culture conditions were: temperature 15°C, light intensity 15μmol photons / (m 2 ·s), the photoperiod is 10L:14D, and the culture medium is natural seawater after filtering, autoclaving and cooling. Take the fresh cladodes of Sargassum in good condition, fully scrub and clean, soak and disinfect in pure water, 75% ethanol, 1% povidone-iodine, 0.01% HgCl, 1% sodium hypochlorite solution for different time, and finally use Rinse with sterile seawater. After dark-adapting the Sargassum algae for 20 min in the dark, the maximum light energy conversion efficiency (Fv / Fm) of photosystem II of algal photosystem II was measured using a modulated chlorophyll fluorescence imaging system (IMAGING-PAM, WALZ, Germany). Prepare Zobell 2216E solid medium, apply the seawater on the surface of Sargassum algae to one side of the solid medium, then crush the algae, spread it on the other side of t...

Embodiment 2

[0019] Antibiotic screening experiment: Take the new branches of Sargassum pre-cultured in the laboratory, wash them thoroughly with sterilized seawater, and culture them in sterilized seawater containing antibiotics for 3 days. The culture conditions are: temperature 15°C, light intensity 15μmol photons / (m 2 s), the photoperiod is 10L:14D, after the end, rinse with sterilized seawater, and spread the plate according to the method described in Example 1, and count the number of colonies. The antibiotic combinations and colony growth conditions are shown in Table 1.

[0020] Table 1 Combination of antibiotics and colony growth

[0021]

[0022]

[0023] Note: + indicates that the corresponding concentration of antibiotics was added.

[0024] According to the number of residual colonies of algae under different antibiotic treatments, the microbial contamination inside and outside the algae without antibiotic treatment is serious; only adding nystatin has no significant i...

Embodiment 3

[0027] Screening of disinfection methods for new shoots of Sargassum: According to the results of Example 1 and Example 2, the best disinfection method was further screened. Select three disinfection steps of pure water, 1% povidone-iodine and antibiotic combination No. 29 in turn, and whether to add 0.01% HgCl 2 to explore further. The scheme is as follows: surface scrubbing-sterile seawater rinsing-1% povidone-iodine soaking for 15s-sterile seawater rinsing-ultrapure water immersion for 60min-sterile seawater recovery-with / without HgCl 2 Soak and disinfect for 5s-Cultivate in sterilized seawater containing antibiotics for 1-3d. The Fv / Fm value of the algae was detected according to the method described in Example 1, and the Zobell2216E solid medium was coated on the third day of treatment to observe the growth of the colony.

[0028] The change of Fv / Fm value of algae and the growth of colony under the two disinfection conditions are as follows: image 3 shown. After dis...

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Abstract

The invention relates to a disinfection treatment method of sargassum thunbergii new branches, and belongs to the field of marine algae culture. The method comprises the steps of taking and cleaning the sargassum thunbergii new branches, soaking algae in 1% povidone iodine for 15 seconds, conducting cleaning with sterilized seawater, and conducting soaking; soaking the algae in sterile pure water for 1 hour, and then cleaning and soaking the algae with the sterilized seawater; and placing the algae in sterilized seawater containing antibiotics, carrying out aerated culture for 3 days under the culture conditions that the temperature is 15 DEG C, the light intensity is 15 mu mol photons / (m<2>.S) and the photoperiod is 10L:14D, and washing the algae with the sterilized seawater to obtain the sterilized sargassum thunbergii new branches. According to the disinfection treatment method of the sargassum thunbergii new branches, microorganisms inside and outside the sargassum thunbergii new branches can be killed, so that the normal growth of the new branches is not affected.

Description

technical field [0001] The invention belongs to the field of seawater algae cultivation, and in particular relates to a method for disinfecting new shoots of Sargassum sargassum. Background technique [0002] Sargassum thunbergii belongs to Phaephyta, Sargassaceae, and Sargassum. As a common sargassum in the intertidal zone, Sargassum and other large brown algae form seaweed beds, which are of great significance for maintaining the balance of offshore ecosystems and environmental restoration. In addition to ecological value, Sargassum also has important economic value and is widely used in feed, medicine, industry, environmental protection and other fields. Sargassum has high nutritional value and can be used as high-quality bait for sea cucumbers and abalones. It is also used in the field of marine medicine because it contains active substances such as lectins and polysaccharides. In addition, Sargassum is an important industrial raw material for extracting polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00A01G7/06A01G13/00A01N59/12A01N43/90A01N43/16A01N47/44A01P1/00
CPCA01G7/06A01G13/00A01G33/00A01N43/16A01N43/90A01N47/44A01N59/12Y02A40/80
Inventor 张朋艳刘福利梁洲瑞袁艳敏汪文俊孙修涛
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI