Enzyme-labeled coupled secondary antibody and preparation method thereof

A technology of coupling and enzyme labeling, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of insufficient antigen sensitivity, loose chain polymer-enzyme-antibody polymer structure, large volume, etc., to achieve good specificity, The effect of high sensitivity and stable structure
CN112649601APending Publication Date: 2021-04-13苏州百道医疗科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
苏州百道医疗科技有限公司
Publication Date
2021-04-13

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Abstract

The invention relates to an enzyme-labelled coupled secondary antibody. The enzyme-labelled coupled secondary antibody is formed by coupling and connecting a modified antibody and a modified labelled enzyme through an oxime bond, wherein the modified antibody is formed by connecting amino of an antibody lysine residue with an amino oxygen group in a modified manner, and the modified labelled enzyme is formed by connecting amino of a lysine residue of an enzyme molecule with aryl aldehyde in a modified manner. The enzyme-labeled coupled secondary antibody is stable in structure, moderate in size, high in sensitivity, good in specificity, free of non-specific staining due to interference of biotin existing in organism tissue, moderate in structure and size, especially suitable for detection of some intracellular antigens, and very high in sensitivity.
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Description

technical field

[0001] The invention relates to the technical field of enzyme-labeled antibodies, in particular to an enzyme-labeled secondary antibody and a preparation method thereof. Background technique

[0002] Immunoassay is a method based on the principle of "antigen-antibody specific binding" to detect proteins in biological samples and then perform qualitative and quantitative analysis. It has the advantages of high sensitivity and specificity. Enzyme-labeled secondary antibodies are widely used in immunoassays, mainly including immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), western blotting (WB), etc.

[0003] (1) There are mainly three types of enzyme-labeled secondary antibodies currently on the market: 1) Antibody-enzyme direct labeling method, that is, a single secondary antibody and HRP enzyme are directly conjugated and labeled with a small molecule reagent. Since labeled enzymes are usually bulky, and the surface area and functional ...

Claims

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