ECL biosensor based on NaBiF4 up-conversion nanoparticles

A biosensor and bacteria detection technology, applied in the field of biosensors, can solve the problems of high cost, environmental pollution, poor sensitivity, etc., and achieve the effects of good stability, high ECL strength and fast response

Active Publication Date: 2021-04-13
HARBIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems of harsh synthesis conditions, high cost, poor

Method used

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  • ECL biosensor based on NaBiF4 up-conversion nanoparticles
  • ECL biosensor based on NaBiF4 up-conversion nanoparticles
  • ECL biosensor based on NaBiF4 up-conversion nanoparticles

Examples

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Embodiment 1

[0032] Step 1. In 10 ml of ethylene glycol (EG) solution, completely dissolve Bi(NO 3 ) 3 ·5H 2 O (0.78mmol), Yb (NO 3 ) 3 ·5H 2 O(0.2mmol), Er(NO 3 ) 3 ·5H 2 O (0.02mmol) and NaNO 3 (2 mmol) to prepare a clear solution 1. Will NH 4 F (14mmol) was added to 25mL EG solution to prepare solution 2;

[0033] Step 2. Slowly add solution 1 dropwise to solution 2 under vigorous stirring, and react with vigorous magnetic stirring at room temperature for 10 minutes to obtain a white emulsion;

[0034] Step 3, after several times of centrifugal cleaning of the above white emulsion, vacuum drying at 60°C to obtain a white powder;

[0035] Step 4. Disperse a certain amount of UCNPs into the mixed solution of Nafion, isopropanol and water (volume ratio is 2:49:49), and the final concentration is 0.5mg mL -1 ;

[0036] Step 5. Continuously polish the exposed glassy carbon electrode with 1.0, 0.3 and 0.05 μm alumina slurry to make the surface smooth, and then wash it with deioni...

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Abstract

The invention discloses an ECL biosensor for detecting pathogenic bacteria, particularly relates to preparation of NaBiF4: Yb<3+>/Er <3+>UCNPs, construction of an ECL immunosensor and ECL detection of escherichia coli O157: H7, and belongs to the technical field of biology/sensors. The invention aims to solve problems of harsh synthesis conditions, high cost, poor sensitivity and high possibility of causing environmental pollution of an up-conversion nano material for ECL detection. The NaBiF4:Yb<3+>/Er<3+> up-conversion nanoparticles which can be synthesized at a room temperature under mild conditions are used for ECL detection of pathogenic bacteria (escherichia coli O157: H7). Gold nanoparticles and an escherichia coli O157: H7 antibody are sequentially assembled on the surface of NaBiF4: Yb<3+>/Er<3+>UCNPs, and the biosensor is optimized to construct the novel ECL immunosensor for detecting the escherichia coli O157: H7. The ECL immunosensor prepared by the invention shows high-sensitivity response to the escherichia coli O157: H7 in a linear range of 200 to 100,000 CFU mL<-1>, and the lowest detection limit is 138 CFU mL<-1>.

Description

technical field [0001] The invention belongs to the technical field of biosensors; the ECL biosensor for pathogenic bacteria detection specifically relates to NaBiF 4 : Yb 3+ / Er 3+ Preparation of UCNPs, construction of ECL immunosensor and ECL detection of Escherichia coli O157:H7. Background technique [0002] Foodborne pathogens contaminate food and drinking water, posing a huge threat to human and animal health. To quickly respond to disease outbreaks caused by foodborne or waterborne pathogens, there is an urgent need to develop new technologies that can detect pathogens quickly and cheaply anywhere. However, due to the low concentration of bacteria in actual samples, many traditional pathogen detection methods require bacterial culture and enrichment steps with a pretreatment time of more than 18 hours. Modern detection methods such as polymerase chain reaction (PCR), surface plasmon resonance (SPR), immunoassays, etc. are much faster, however, these methods either...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N27/327G01N21/76
CPCG01N33/56916G01N21/76G01N27/3278G01N2333/245Y02A50/30
Inventor 刘丹青吕星星赵超越黄金美郭英
Owner HARBIN UNIV OF SCI & TECH
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